| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,703,062 | T→A | 15.4% | R629R (CGT→CGA) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,703,062 | 0 | T | A | 15.4% | 575.8 / 21.3 | 351 | R629R (CGT→CGA) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base T (105/156); new base A (45/5); total (163/188) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 353 total aligned reads displayed.
GCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGC > CP009273/1702997‑1703128 | gCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCAGTAGAAGCCGCTATTGCCCGAGc > 1:7035110/1‑68 (MQ=11)gCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg > 1:19724309/1‑67 (MQ=35) cTAATGCGGAACCAGAAGACCAGGGCGTTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg < 1:10066080/66‑1 (MQ=11) cTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTg < 1:22890948/66‑1 (MQ=25) aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa < 1:13827729/68‑1 (MQ=35) aTGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa < 1:29802697/68‑1 (MQ=35) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCGGCCGTAGAAGCGGCTATTGCCCGCGCGAAGGc > 1:16613349/1‑68 (MQ=11) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTGCCCGAGCCAAAGc > 1:9055033/1‑68 (MQ=11) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCGaatg > 1:10187896/1‑65 (MQ=255) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAg > 1:29173220/1‑67 (MQ=35) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAg > 1:27015310/1‑67 (MQ=35) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGc > 1:30218977/1‑68 (MQ=35) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGc > 1:33785490/1‑68 (MQ=25) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTAGAAGCCGCTATTGCCCGAGCAAAAGc > 1:31314229/1‑68 (MQ=11) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTCGAAGCCGCTATTGCCCGAGCAAAGGc > 1:26417266/1‑68 (MQ=11) gCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCCGCTATTGCCCGAGCAAAAg > 1:7912416/1‑67 (MQ=11) aCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCa > 1:31084111/1‑68 (MQ=255) ccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCCAAGCCGCTATTGCCCGAGCCAAAGCACGCaa > 1:8375148/1‑68 (MQ=255) cagcagAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg < 1:35166327/68‑1 (MQ=255) cAGAATAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg < 1:2517782/68‑1 (MQ=255) cAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg < 1:34166925/68‑1 (MQ=40) aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTg > 1:25692304/1‑68 (MQ=40) ccaaCATGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAg < 1:24341330/62‑1 (MQ=11) caaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGa < 1:10678870/67‑1 (MQ=18) caaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGa < 1:22419435/67‑1 (MQ=18) aCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGaa < 1:5606394/67‑1 (MQ=35) tgccGATCCTCGCAAACCAGCCGTCGAAGCAGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGc < 1:12431811/65‑1 (MQ=11) tgccGATCCCCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGc < 1:9837784/65‑1 (MQ=11) tgTCGATCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGc < 1:613716/67‑1 (MQ=11) ggcGATCCTCGCAAATCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCa < 1:1314896/66‑1 (MQ=11) gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCa > 1:3306421/1‑68 (MQ=25) tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGc < 1:17883396/66‑1 (MQ=35) tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCa < 1:13580774/67‑1 (MQ=35) tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCa < 1:17045408/67‑1 (MQ=35) tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCa > 1:11190971/1‑67 (MQ=35) tCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCa > 1:38568059/1‑67 (MQ=25) gcGATCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGc < 1:20794152/65‑1 (MQ=11) ccGATCCTCGAAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGc < 1:6660765/65‑1 (MQ=11) aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAg > 1:1536362/1‑68 (MQ=25) tCCTCGTAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:27442313/68‑1 (MQ=11) tCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:16398511/68‑1 (MQ=25) tCCTCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:22611719/68‑1 (MQ=11) tCCGCGTAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:14686892/68‑1 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:23456793/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:12869965/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:24050305/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:33556033/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:33031054/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:37941586/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:20297807/68‑1 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAg > 1:21251354/1‑67 (MQ=35) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAg > 1:36837617/1‑67 (MQ=25) tCCGCGCAAACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAgg < 1:27764910/68‑1 (MQ=25) ccGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGc < 1:23260484/68‑1 (MQ=35) aaaGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATg < 1:36223204/67‑1 (MQ=35) aaaCCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATg < 1:16737708/67‑1 (MQ=255) gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa > 1:35914728/1‑68 (MQ=40) gccgccGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa > 1:34706372/1‑68 (MQ=40) gccgccGTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGcgg > 1:7286269/1‑65 (MQ=9) ccgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:22095141/68‑1 (MQ=255) ccgccgTCGAAGCCGCTATTGCCCGTCCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:36952335/68‑1 (MQ=255) ccgccgTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:24679519/68‑1 (MQ=255) ccgccgTCGAAGCAGCTATCGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:18920718/68‑1 (MQ=255) cagccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:4781189/66‑1 (MQ=255) cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:11446141/67‑1 (MQ=255) cgccgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAg < 1:19399014/67‑1 (MQ=255) cgTGGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa < 1:22232093/67‑1 (MQ=255) cgTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa < 1:18288115/67‑1 (MQ=255) cgTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa < 1:28779448/67‑1 (MQ=255) cgTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAACCTGGAACAGCAACAGGCTAATGCCGAGCCa < 1:32162066/67‑1 (MQ=14) cgTCGAAGCAGCTATCGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCa < 1:17366321/67‑1 (MQ=255) cGAAGCCGCTATTGCCCGTGCCAAAGCCGGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa < 1:20144734/67‑1 (MQ=255) cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:21594822/68‑1 (MQ=255) cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:19580305/68‑1 (MQ=255) cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac > 1:38033496/1‑68 (MQ=255) cGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGaac > 1:16198045/1‑68 (MQ=255) cGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaac < 1:10951114/68‑1 (MQ=255) gAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca < 1:32514459/68‑1 (MQ=255) gCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGGGCCAGCacaaca > 1:18651214/1‑68 (MQ=255) ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg > 1:19501588/1‑68 (MQ=255) ccGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg > 1:4407548/1‑68 (MQ=255) agCTATTGCCCGGGCCATAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAg < 1:37045406/66‑1 (MQ=14) tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGtag > 1:8138325/1‑66 (MQ=255) atcgCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:14574103/65‑1 (MQ=255) aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTc > 1:6493806/1‑66 (MQ=255) aTTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGa < 1:6104354/68‑1 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:13096448/1‑67 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:5370055/1‑67 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:26843454/1‑67 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATcc > 1:23640960/1‑67 (MQ=255) cccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTAGATcc > 1:39124304/1‑67 (MQ=255) ccGTGCCAAAGCACGCAAGCTGGAACTGCAACAGTGTAATGCCGATCAAGAACAACAGGTCGATCcgc < 1:7223488/68‑1 (MQ=14) ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTGGATAcgc > 1:866762/1‑68 (MQ=14) ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:14242815/1‑68 (MQ=255) ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:25077128/1‑68 (MQ=255) ccGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc > 1:24621248/1‑68 (MQ=255) ccGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc < 1:37307677/68‑1 (MQ=255) ccGCGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc < 1:28272249/68‑1 (MQ=255) ccGCGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgc < 1:4142156/68‑1 (MQ=255) gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCgcgc > 1:14334050/1‑68 (MQ=255) | GCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGC > CP009273/1702997‑1703128 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |