| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 1,703,187 | C→A | 6.1% | P671Q (CCG→CAG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 1,703,187 | 0 | C | A | 6.1% | 916.9 / 6.9 | 314 | P671Q (CCG→CAG) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (140/151); new base A (2/21); total (142/172) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 318 total aligned reads displayed.
GATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGC > CP009273/1703120‑1703254 | aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCg > 1:32714271/1‑68 (MQ=255) tCCTCGTAAATCCGCCGTCGAAGCCGCTATAGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:38236235/68‑1 (MQ=17) tCCTCGTAAACCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:26274862/68‑1 (MQ=14) tCCTCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:16232624/68‑1 (MQ=255) tCCTCGCAAACCAGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCg < 1:31451036/67‑1 (MQ=17) tCCGCGCTAAGCCGACGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:14792894/68‑1 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAGCCg > 1:8576156/1‑67 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:29596854/68‑1 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:29336867/68‑1 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:15188625/68‑1 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCg > 1:13979798/1‑67 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCg > 1:34224300/1‑67 (MQ=255) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAATCGGCAACAGCAACCgg > 1:2902310/1‑68 (MQ=14) tCCGCGCAAAGCCGCCGTCGAAGCCGCTATAGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:3355187/68‑1 (MQ=255) tCCGCGAAAAGCCTCCGTCGAAGACGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:4062375/68‑1 (MQ=17) tCCCCGAAAATCTGCCGTCGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCgg < 1:10004399/68‑1 (MQ=17) ccGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAGCCg > 1:36539065/1‑66 (MQ=255) ccGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGc < 1:21485079/68‑1 (MQ=255) cgcgCAAAGCCGCCGTCGACGCCGCTATTGCCCGCGCCAACGCGCGCAAACGGGACCAGCAACCGGCt > 1:23786532/1‑68 (MQ=14) gcAAAGCCGCCGTCGAATCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAAt < 1:18692429/68‑1 (MQ=255) gccgccGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa > 1:33640613/1‑68 (MQ=255) gccgccGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa > 1:26510521/1‑68 (MQ=255) cccgccgTCGAAGCCGCTATTGCCCGCCCCAACGCGCGCAACCGGGAACAGCAACCGGCTAATGCGGa < 1:20142847/67‑1 (MQ=14) ccgccgTAGAAGCAGCTATCGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAg < 1:17552144/68‑1 (MQ=14) tgccgTCGAAGCAGCTATCGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGc < 1:30959621/67‑1 (MQ=17) cgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAg < 1:30515591/67‑1 (MQ=255) cgTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCa < 1:9015937/67‑1 (MQ=255) cgTCGAAGCAGCTACTGCCCGAGCCAAAGCGCGCATACGGGAACCGCAACCGGCTAATGCGGAGCCa < 1:26643660/67‑1 (MQ=18) cgTAGAAGCCGCTATAGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCa < 1:37358680/67‑1 (MQ=255) gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga < 1:24264933/68‑1 (MQ=255) gTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCaga < 1:23241890/68‑1 (MQ=255) cGATGCAGCTATTGCCCGAGCCAATGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaag < 1:22628893/68‑1 (MQ=18) cGAAGCAGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaag < 1:12656057/68‑1 (MQ=255) aGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAc > 1:33367819/1‑68 (MQ=255) cggCTATTTCACGAGACAAAGAGCGAAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg < 1:3742655/66‑1 (MQ=18) ccGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg > 1:17637237/1‑68 (MQ=255) ccGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg > 1:24456411/1‑68 (MQ=255) ccGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg > 1:19876610/1‑68 (MQ=255) ctcTATTGCCAGAGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg < 1:2131872/65‑1 (MQ=17) cGCTATTGCCCGCGCCAAAGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg < 1:7107232/67‑1 (MQ=255) cGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACa < 1:35329193/66‑1 (MQ=255) agCTATCGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAg < 1:25176105/66‑1 (MQ=255) aTTGCTCGAGCCATAGAGGGCTAACTGTAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGa < 1:25647363/68‑1 (MQ=14) aTTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGa < 1:18879816/68‑1 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:32262099/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:11708850/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:12951463/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:19297525/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:15124576/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:34591359/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATcc > 1:22069951/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGATGATcc > 1:5718151/1‑67 (MQ=255) cccGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGGCAGAAGATCAGGATGATcc > 1:27661307/1‑67 (MQ=17) cctaGCCTAAGCGCGCAACCGTTAACATCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc < 1:8740975/65‑1 (MQ=17) ccGAGCCAAAGCGCGCAATCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc < 1:7456664/68‑1 (MQ=255) ccGAGCCAAAGCGCGCAATCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc < 1:22833829/68‑1 (MQ=255) ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc > 1:8272156/1‑68 (MQ=255) ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc > 1:915719/1‑68 (MQ=255) ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc > 1:5203197/1‑68 (MQ=255) ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgc > 1:29455541/1‑68 (MQ=255) ccGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGATGATCcgc > 1:27566343/1‑68 (MQ=255) ccAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaaa > 1:11123248/1‑68 (MQ=255) ccAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCaaa > 1:35515995/1‑68 (MQ=255) aaaGCGCGCAAACTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGc < 1:31105521/68‑1 (MQ=255) aaaGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGc < 1:27045185/68‑1 (MQ=255) aGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCt > 1:10694505/1‑67 (MQ=255) gcgcgcAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTg > 1:18431141/1‑67 (MQ=255) gcgcAATCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGcc < 1:37214827/67‑1 (MQ=11) cGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc > 1:8222062/1‑68 (MQ=255) tggAACAGCAACCGGCTAATGCGGACCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGGCGAAGCg < 1:16665369/67‑1 (MQ=14) tggAACAGCAACAGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg < 1:31326484/67‑1 (MQ=11) tggAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg < 1:30289612/67‑1 (MQ=255) gggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg < 1:14242685/68‑1 (MQ=255) gggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg < 1:6141388/68‑1 (MQ=255) gggAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg < 1:15466896/68‑1 (MQ=255) gggAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCg < 1:10557799/68‑1 (MQ=255) ggAACAGCAACCGGCTAATGCGGACCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCgg < 1:3240582/68‑1 (MQ=255) caCAGCAACCGGCTCATGCTGAGCCAGATCAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:13478837/66‑1 (MQ=11) caCAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTGGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:35938104/66‑1 (MQ=14) aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:448601/67‑1 (MQ=255) aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:4681738/67‑1 (MQ=255) aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:34392001/67‑1 (MQ=255) aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:39106581/67‑1 (MQ=255) aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:20990865/67‑1 (MQ=255) aaCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:23734735/67‑1 (MQ=255) aaCAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGc < 1:26831506/67‑1 (MQ=255) tcAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCt < 1:1216704/66‑1 (MQ=255) acacCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCt < 1:27795559/67‑1 (MQ=255) aCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa > 1:15740732/1‑68 (MQ=255) aCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa > 1:25087637/1‑68 (MQ=255) aCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa > 1:13220479/1‑68 (MQ=255) aCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa > 1:34942972/1‑68 (MQ=255) aCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa > 1:16042483/1‑68 (MQ=255) aCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTAGACGCGGCTa > 1:7264709/1‑68 (MQ=14) aCAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCt < 1:31791343/67‑1 (MQ=255) aCAGCAACAGGCTAATGCGGACCCAGAACAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCt < 1:21754787/67‑1 (MQ=11) aGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTAtt > 1:17198712/1‑68 (MQ=255) aGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt > 1:37915304/1‑68 (MQ=255) aGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt > 1:5963599/1‑68 (MQ=255) aGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAtt > 1:32526192/1‑68 (MQ=255) | GATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGC > CP009273/1703120‑1703254 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |