BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	691,928	G→A	5.7%	noncoding (33/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	691,928	0	G	A	5.7%	1077.6 / 30.3	345	noncoding (33/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (85/231); new base A (2/17); total (88/257)
Fisher's exact test for biased strand distribution p-value = 1.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 347 total aligned reads displayed.

TCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAA  >  CP009273/691862‑691992

TCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGa                                                                 >  1:36530628‑M1/1‑68 (MQ=255)

    cGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATc                                                              <  1:7685832/67‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:18875760/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:20984894/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:3059255/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:33303958/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:5608023/68‑1 (MQ=255)

       aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          <  1:5930896/68‑1 (MQ=255)

         cctgctttttTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:36244164‑M2/58‑1 (MQ=255)

         cctgctttttTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:20824669‑M2/58‑1 (MQ=255)

          gtgctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:21715447‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:12370451‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:38915970‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:3166918‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:29365255‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:26866710‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:20237058‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:22983383‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:17229347‑M2/59‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:11139416‑M2/58‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:38399853‑M2/58‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:20592656‑M2/58‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:24198107‑M2/58‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:25642845‑M2/58‑1 (MQ=255)

          gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:14689057‑M2/58‑1 (MQ=255)

           agctttatTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:1162632‑M2/59‑1 (MQ=255)

               ttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                  <  1:25139774‑M2/64‑1 (MQ=255)

               ttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                  <  1:36648162‑M2/64‑1 (MQ=255)

                 ttTAATGTGGCTGGGGTACGTGGATTCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGc                                                <  1:2778431‑M2/66‑1 (MQ=255)

                 ttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                <  1:34039402‑M2/66‑1 (MQ=255)

                 ttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                <  1:17849488‑M2/66‑1 (MQ=255)

                  tTAATGTGGCTGGGGTACGAGGATTCGAACCTCTGAATGCAGGAATAAGAATCAGGTGCCTTAACGCt                                               >  1:3458180‑M2/2‑68 (MQ=255)

                  tTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               <  1:37557407‑M2/67‑1 (MQ=255)

                     aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:7982865/68‑1 (MQ=35)

                     aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                            <  1:21845767/68‑1 (MQ=35)

                     aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTgg                                            <  1:6315880/68‑1 (MQ=255)

                      tgtgGCTGGGGTACGAGGATTCGAACCTGGGAATTAAGGAATAATACTCCCCTGCGTTACAGCTTgga                                           >  1:15079807/1‑67 (MQ=2)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:35260/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:35420555/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:15876025/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:30735658/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:3729497/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:26299204/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:6555409/68‑1 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:17930088/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:38689594/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:29127008/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:12669007/1‑68 (MQ=33)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCATgtc                                           >  1:33934599/1‑66 (MQ=255)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTGGc                                           <  1:26767691/68‑1 (MQ=255)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGc                                           <  1:22590434/68‑1 (MQ=255)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:16309029/1‑68 (MQ=18)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGACGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:5206536/1‑68 (MQ=18)

                      tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGACGCAATCAGAATACCGTGCCTTACCTCTTCGc                                           >  1:13684690/1‑68 (MQ=11)

                      agtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:16819702/67‑1 (MQ=14)

                                    gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                             <  1:38536480/68‑1 (MQ=35)

                                    gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                             <  1:39419505/68‑1 (MQ=35)

                                    gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                             <  1:4282289/68‑1 (MQ=35)

                                     aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaataa                              >  1:10562073/1‑63 (MQ=1)

                                     aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACtacg                            >  1:39232143/1‑65 (MQ=14)

                                     aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgtg                            >  1:1995058/1‑66 (MQ=18)

                                       gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTAGGTg                          >  1:7143863/1‑68 (MQ=25)

                                        aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                          <  1:11381188/67‑1 (MQ=40)

                                        aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                          <  1:17075917/67‑1 (MQ=40)

                                         ttCGAACCTCGGCATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                         <  1:28593070/67‑1 (MQ=255)

                                         ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGc                         <  1:8724736/67‑1 (MQ=255)

                                         ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                        <  1:4387643/68‑1 (MQ=255)

                                         ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCa                        <  1:6163247/68‑1 (MQ=255)

                                          tCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAc                       <  1:6908671/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGATTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCTACTGGGTGCACt                      <  1:13326174/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:27464704/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:21321647/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:38344344/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:29774745/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:23378383/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:36970368/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:13170501/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:35747467/68‑1 (MQ=255)

                                           cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:32162391/68‑1 (MQ=255)

                                           cGAACCGCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                      <  1:24697533/68‑1 (MQ=255)

                                            gAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACtt                     <  1:1200572/68‑1 (MQ=255)

                                              aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                    <  1:14345528/67‑1 (MQ=255)

                                              aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                    <  1:28376134/67‑1 (MQ=255)

                                              aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTa                    <  1:34809963/67‑1 (MQ=255)

                                               ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                  <  1:38986178/68‑1 (MQ=255)

                                               ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                  <  1:9444619/68‑1 (MQ=255)

                                                 tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                <  1:3116504/68‑1 (MQ=255)

                                                 tCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAg                <  1:35968421/68‑1 (MQ=255)

                                                  cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAgg               <  1:37072906/68‑1 (MQ=255)

                                                     aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa             <  1:32489657/67‑1 (MQ=255)

                                                        gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCg         <  1:33007031/68‑1 (MQ=255)

                                                          cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt        <  1:15559281/67‑1 (MQ=255)

                                                          cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt        <  1:19306381/67‑1 (MQ=255)

                                                          cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt        <  1:8272116/67‑1 (MQ=255)

                                                          cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt        <  1:13917556/67‑1 (MQ=255)

                                                          cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt        <  1:9127434/67‑1 (MQ=255)

                                                              aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCCCTTACAAGGTAAGCGTCTTGa   <  1:28331304/68‑1 (MQ=255)

                                                              aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg    <  1:22113039/67‑1 (MQ=255)

                                                              aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa   <  1:39551576/68‑1 (MQ=255)

                                                               tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGaa  <  1:18594291/68‑1 (MQ=255)

TCGCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAA  >  CP009273/691862‑691992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`