BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	691,931	T→A	6.6%	noncoding (30/75 nt)	glnX ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	691,931	0	T	A	6.6%	1137.5 / 21.1	353	noncoding (30/75 nt)	glnX	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (79/248); new base A (4/22); total (83/270)
Fisher's exact test for biased strand distribution p-value = 4.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

GCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATT  >  CP009273/691864‑691998

GCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     >  1:11653577‑M1/1‑68 (MQ=255)

 CCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAt                                                                     <  1:33599831‑M1/67‑1 (MQ=255)

   gAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCg                                                                  >  1:38388448/1‑68 (MQ=255)

     aGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                <  1:5930896/68‑1 (MQ=255)

       cgagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:38276884‑M2/11‑67 (MQ=255)

       cctgctttttTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:20824669‑M2/58‑1 (MQ=255)

        gagctttttTAATTTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:21887854‑M2/59‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:32119227‑M2/59‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGc                                                              <  1:28354669‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               <  1:8742179‑M2/57‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:22983383‑M2/59‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:14297332‑M2/59‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:20237058‑M2/59‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:14882153‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:33094584‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:20592656‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:30690597‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:38888759‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:11139416‑M2/58‑1 (MQ=255)

        gagctttttTAATGTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:35312120‑M2/59‑1 (MQ=255)

        cagctttttTAAAGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:4810066‑M2/58‑1 (MQ=255)

              tttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGcc                                                             <  1:21755153‑M2/59‑1 (MQ=255)

              ttatAATGTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGc                                                              <  1:26391289/58‑1 (MQ=11)

               ttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:21060405‑M2/66‑1 (MQ=255)

               ttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:25878067‑M2/66‑1 (MQ=255)

               ttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:17849488‑M2/66‑1 (MQ=255)

               ttTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:17442686‑M2/66‑1 (MQ=255)

                tTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:12514207‑M2/2‑68 (MQ=255)

                   aTGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAATCCGGTGCCTTACCGCTTgg                                                  <  1:28721078/68‑1 (MQ=25)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:34825765/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:37556943/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:38532844/68‑1 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:38689594/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:39539973/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:626685/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:30014954/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:29566179/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:6946397/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:26835918/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:8070843/68‑1 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:9472514/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:25110029/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:25584850/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:12845008/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:13456002/68‑1 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:14563398/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:1567928/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:15876025/68‑1 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:18165799/1‑68 (MQ=33)

                    tgtgGCTGGGGTACGAGGATTCGAACCTCGGAATGACGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:5206536/1‑68 (MQ=18)

                    tgtgGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGc                                                 <  1:28047223/68‑1 (MQ=11)

                     gtgGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCATAATCCGGTGCCTTACCGCTTGGCg                                                <  1:27217939/68‑1 (MQ=14)

                                  gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                   <  1:23436600/68‑1 (MQ=35)

                                  gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                   <  1:4282289/68‑1 (MQ=35)

                                  gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgg                                   <  1:39419505/68‑1 (MQ=35)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaataa                                    >  1:10562073/1‑63 (MQ=1)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTgtg                                  >  1:1995058/1‑66 (MQ=18)

                                   aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTggg                                  >  1:31332812/1‑68 (MQ=37)

                                     gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGCGt                                 >  1:16367689/1‑67 (MQ=18)

                                     gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAGGGt                                 >  1:36630291/1‑67 (MQ=14)

                                      aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                <  1:36904705/67‑1 (MQ=40)

                                      aTTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTg                                <  1:17075917/67‑1 (MQ=40)

                                       ttCGAACCTCGGAATGCGGGAATCTTAATCCGGTGCCTTACGGCTTGGCGATACCCCTACTGGGTGCa                              <  1:36395585/68‑1 (MQ=14)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:30885166/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:37277534/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:16853423/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:21321647/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:22318515/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:28582333/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:13246070/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:6423210/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:783970/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:24692541/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:35995615/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:3576657/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:34513763/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACt                            <  1:37061915/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTACACt                            <  1:27620709/68‑1 (MQ=255)

                                         cGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGATGCACt                            <  1:137928/68‑1 (MQ=255)

                                             ccgcGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                        <  1:6666489/65‑1 (MQ=255)

                                             ccTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACa                        <  1:38986178/68‑1 (MQ=255)

                                              cTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACaa                       >  1:11492666/1‑68 (MQ=255)

                                                   aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTa                   <  1:3545996/67‑1 (MQ=255)

                                                      gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCg               <  1:2914962/68‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:3394798/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:9127434/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:8272116/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:19306381/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:18883060/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:5842066/67‑1 (MQ=255)

                                                        cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGt              <  1:39227591/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg          <  1:22702343/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg          <  1:12195632/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTg          <  1:321942/67‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa         <  1:32786756/68‑1 (MQ=255)

                                                            aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGa         <  1:34596391/68‑1 (MQ=255)

                                                             tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGaa        <  1:18594291/68‑1 (MQ=255)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACATGGTAAGCGTCTTGAATaa     <  1:15190047/66‑1 (MQ=255)

                                                                  aTCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATa      <  1:23933418/65‑1 (MQ=255)

                                                                   tCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCAGTTACAATTTAAGCGTCTTGAATaaagt  >  1:7336010/1‑66 (MQ=25)

GCCGAAGCGAGCTTTTTTAATGTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACTGGGTGCACTTACAAGGTAAGCGTCTTGAATAAATT  >  CP009273/691864‑691998

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`