BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,040	G→A	5.3%	noncoding (33/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,040	0	G	A	5.3%	1066.7 / 23.3	365	noncoding (33/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (244/91); new base A (9/10); total (258/107)
Fisher's exact test for biased strand distribution p-value = 3.27e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 376 total aligned reads displayed.

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTC  >  CP009273/691973‑692104

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                  >  1:24069678/1‑68 (MQ=255)

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                  >  1:12083732/1‑68 (MQ=255)

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                  >  1:17888190/1‑68 (MQ=255)

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                  >  1:15975662/1‑68 (MQ=255)

  cAAGGTAAGCGTCTTGAATAAATTCCCTGGCCTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:16337717/1‑68 (MQ=21)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCaaaa                                                                >  1:29240034/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:8010837/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:25081754/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:37509843/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:34390196/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:3698483/1‑67 (MQ=255)

     ggTAAGCGTCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:16853125/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCTGAATGACGGAATCAGAAtac                                                             >  1:33594224/1‑66 (MQ=21)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:6847274/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:18991154/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:19772219/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:4704076/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:6184228/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:5690548/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:2289720/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:24683573/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:37108334/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:14011126/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:34099809/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAAcc                                                             >  1:7436102/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAAcc                                                             >  1:19281857/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCaaaaa                                                               >  1:39457230/1‑63 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:39090164/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:21610373/1‑68 (MQ=255)

         aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                          >  1:36638881/1‑67 (MQ=255)

          ccctCTTGAATAAATGGGCTGGGGTACGAGGATTCGAACCTCGGAAGGCCGGAATCAGAATCCGGTGc                                                        <  1:23744971/65‑1 (MQ=17)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:29726017/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:11759963/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:27687259/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:22737304/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:8702130/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:29873395/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:30746532/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:36155766/1‑66 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATCACTGAATGATAATCCtgtg                                                         >  1:38615375/1‑66 (MQ=17)

            ggcTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:22684837/65‑1 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGc                                                        <  1:33379997/66‑1 (MQ=17)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                      >  1:38368589/1‑68 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                      >  1:34768350/1‑68 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                      >  1:9321795/1‑68 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAAAGCCGGAATCAGAATCCGGTGccg                                                      >  1:4159387/1‑67 (MQ=255)

            gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGc                                                        <  1:18218529/66‑1 (MQ=11)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:31749604/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:14692952/1‑68 (MQ=255)

                tGAATAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTAcc                                                  <  1:16546629/68‑1 (MQ=14)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTAcctgt                                               >  1:5038066/1‑65 (MQ=14)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:15124140/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:20887449/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:14248714/1‑68 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTGCCTTACCGcg                                               >  1:32077795/1‑67 (MQ=255)

                      aaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTgg                                            <  1:37869718/68‑1 (MQ=255)

                       tttGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCACAATCCGGTGCCTTACCGCTTGGc                                           <  1:26322546/67‑1 (MQ=11)

                       tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGc                                           <  1:10722257/67‑1 (MQ=11)

                       aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGc                                           <  1:27866518/68‑1 (MQ=11)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAATCCGGTGCCTTACCGCCTgcct                                          >  1:19110421/1‑65 (MQ=11)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:2657474/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:23798450/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:4025070/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:15719361/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:8530812/1‑68 (MQ=31)

                                   aagAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                               <  1:15376780/66‑1 (MQ=14)

                                   aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                               <  1:32710631/68‑1 (MQ=31)

                                   aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                               <  1:32971799/68‑1 (MQ=31)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggg                            >  1:7147043/1‑65 (MQ=1)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaatt                            >  1:19486328/1‑66 (MQ=18)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaagt                            >  1:35439863/1‑66 (MQ=18)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:35846332/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:8223283/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:5404595/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:11946571/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:25196658/1‑68 (MQ=37)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAAACCGGTTCCTTAAAGCTAGGCGAAACCCcaacaaca                            >  1:21324565/1‑66 (MQ=11)

                                       ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                           >  1:20578495/1‑68 (MQ=39)

                                       ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                           >  1:23279762/1‑68 (MQ=39)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                        <  1:31483694/68‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                        <  1:13180912/68‑1 (MQ=255)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                        <  1:25867599/68‑1 (MQ=255)

                                              aaCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTg                    >  1:9775970/1‑68 (MQ=255)

                                               aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                   >  1:12459002/1‑68 (MQ=255)

                                                 cTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTGTGAAt                 >  1:7602981/1‑68 (MQ=255)

                                                   cGGAATGCCGGAATCAGATTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt               <  1:10481282/68‑1 (MQ=255)

                                                   cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt               <  1:18343376/68‑1 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa          <  1:28909101/68‑1 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa          <  1:9035436/68‑1 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa          <  1:16264251/68‑1 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa          <  1:17626858/68‑1 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa         <  1:19935367/68‑1 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa         <  1:22011169/68‑1 (MQ=255)

                                                         gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa         <  1:10107970/68‑1 (MQ=255)

                                                           cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc        <  1:28779574/67‑1 (MQ=255)

                                                            tgAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat      <  1:20330222/67‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata     <  1:5894656/67‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata     <  1:15802616/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:18550064/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:33750593/67‑1 (MQ=255)

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTC  >  CP009273/691973‑692104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`