| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,300,453 | T→G | 10.2% | N256T (AAC→ACC) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,300,453 | 0 | T | G | 10.2% | 1863.8 / 48.4 | 738 | N256T (AAC→ACC) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base T (353/301); new base G (82/2); total (435/303) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.88e-18 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 790 total aligned reads displayed.
GTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTT > CP009273/3300386‑3300519 | gTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGt > 1:30072026/1‑68 (MQ=255)gTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGt > 1:35345855/1‑68 (MQ=255)gTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCACAAGAGCTTCAGCCACTTCCAGAGTCGCGt > 1:14629088/1‑68 (MQ=255) tCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt > 1:26900544/1‑68 (MQ=255) tCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtt > 1:776992/1‑68 (MQ=255) aCCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGtttt > 1:28115103/1‑68 (MQ=255) ccGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGttttt > 1:36338990/1‑68 (MQ=255) cGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTg > 1:10173832/1‑68 (MQ=255) cGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTg > 1:16672785/1‑68 (MQ=255) gTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGgtttttg > 1:14119550/1‑66 (MQ=255) ttCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTggg > 1:7323583/1‑67 (MQ=255) ttCAGCGAGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:8474166/1‑68 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:31765265/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt < 1:17716054/67‑1 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:5421219/1‑67 (MQ=255) gcgcGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacga > 1:37276287/1‑68 (MQ=255) gcgcGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacga > 1:38850108/1‑68 (MQ=255) ggggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:27706797/65‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTacgaac > 1:11435284/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgcac > 1:31241092/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:7023803/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:34506596/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:4860737/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:37997622/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:21886058/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:5992708/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACGTCCAGAGTCGCGTTTTTGGTacgaac > 1:17168900/1‑68 (MQ=255) gcggcgCTGTTGTAGCAGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTacgaac > 1:13518346/1‑68 (MQ=255) gcgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaacga < 1:8183073/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:10964845/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:2902228/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:87179/67‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:27500415/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:13840163/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:6298044/68‑1 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGCACGAAGATaaaa > 1:25132167/1‑66 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGAAAAt > 1:36023461/1‑67 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGAACGAAGATAAt > 1:36722457/1‑67 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGCACCAAGATAATcgc > 1:28677410/1‑67 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgcc > 1:33919417/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTTAGAACGAAGATAATagccgc > 1:24735995/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGTTTATagccgc > 1:21431238/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGGACGAAGATAATcgccgc > 1:26314941/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGCACGAAGATAATagccgc > 1:19734832/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGAACGAAGATAATcgccgc > 1:20025116/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAATATAATggccg > 1:18473531/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATTATcgccgc > 1:9020557/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:32905582/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:37601221/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:35839561/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:39301/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:9752276/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:26784431/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:11913222/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:20607551/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATagccgc > 1:33283638/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAAAGAAGATAATcgccg > 1:20334014/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGAACGAAGATAAAcgccgc > 1:29240459/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTCGCACGCAGATAATagccgc > 1:12449551/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTCGAACGAAGATAATcgccgc > 1:16923623/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGGACGAAGATAATagccgc > 1:1952878/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGACGAACCCAGATAATcgccc > 1:24071546/1‑66 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:25633083/68‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:30498065/68‑1 (MQ=255) gTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACTAACGAAGATAATCGCCGCAt < 1:24250211/68‑1 (MQ=255) ttACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGACGAACGAAGATAATAGCCGCATc > 1:22653083/1‑68 (MQ=255) tACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATCGCCGCATCa > 1:15546471/1‑68 (MQ=255) gCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGCACGAAGATTATCGCCGCATCaaaa > 1:19205196/1‑68 (MQ=255) gCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaaaa > 1:34236927/1‑68 (MQ=255) gCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCaaaa > 1:37004419/1‑68 (MQ=255) gCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTTCGCACGAAGATAATCGCCGCATCaaaa > 1:30767705/1‑68 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTGCGAACGCAGATAATCGCCGCATCAAAATAtt > 1:31464712/1‑67 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGCACGAAGATAATAGCCGCCTCAAAATCtt > 1:4215495/1‑67 (MQ=255) agCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCAACAAAATCTTcc > 1:38287041/1‑67 (MQ=255) agCTTCAGCCACTTCCAGAGTCGCGTGTTTGGTTCGGACGTAAATAATCGCCGCAACAAAAACTTcag > 1:16369358/1‑66 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATATTcc > 1:23584436/1‑66 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAAACGCCGCATCAAAATATTcc > 1:34787478/1‑66 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATAttac > 1:6539101/1‑64 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt < 1:14448266/67‑1 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:32656442/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:13276528/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:28417532/68‑1 (MQ=255) aGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTACGCTTCCa > 1:21530535/1‑68 (MQ=255) gCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCATCAAAATATTCCGCTTCCAg > 1:35032974/1‑68 (MQ=255) ccACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAgg > 1:9491716/1‑68 (MQ=255) ccACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCGTCCAgg > 1:22052195/1‑68 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGa < 1:20920645/68‑1 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGa < 1:5705705/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:18109644/68‑1 (MQ=255) tCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACg < 1:12840378/68‑1 (MQ=255) cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa < 1:1741745/68‑1 (MQ=255) cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa < 1:25934578/68‑1 (MQ=255) cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa < 1:15035654/68‑1 (MQ=255) agagTCGCGTTTTTTGTACGAACGAAGATAATAGCCGCATCAAAATATTACGCTTCCAGGAAACCTa > 1:11713486/1‑67 (MQ=255) agTCGCGTTTTTGGTACGAACGCAGATAATCGCCGCATCAAAATATTACGCGTCCACGAAACGTACCa > 1:15919424/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTACGCTTCCAGGAAACGTAcaa > 1:21236284/1‑66 (MQ=255) agTCGCGTTTTTGGTACGAACCAAGATAATAGCCGCATCAAAAAATTACGCTTCCAGGGAACCTACCa > 1:23301628/1‑68 (MQ=255) gTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTACAGGAAAAGTACCAg > 1:32224901/1‑68 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTg < 1:10465805/68‑1 (MQ=255) gTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCt > 1:18897921/1‑67 (MQ=255) | GTCACCGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTT > CP009273/3300386‑3300519 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |