| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,300,458 | G→T | 6.8% | T254T (ACC→ACA) | deaD ← | ATP‑dependent RNA helicase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,300,458 | 0 | G | T | 6.8% | 2149.7 / 22.0 | 736 | T254T (ACC→ACA) | deaD | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base G (367/309); new base T (56/2); total (424/312) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.57e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 758 total aligned reads displayed.
CGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTT > CP009273/3300391‑3300525 | cGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTg > 1:10173832/1‑68 (MQ=255) gTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTgg > 1:34146780/1‑68 (MQ=255) gTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTgg > 1:18069774/1‑68 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGt > 1:26332675/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:6018471/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt < 1:17670025/67‑1 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:3045416/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGt > 1:21090421/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTTGt > 1:14104719/1‑67 (MQ=255) tCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTATGGTa > 1:18763318/1‑68 (MQ=255) ggggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:27706797/65‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGCGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTtcgca > 1:31405553/1‑65 (MQ=38) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTacgaac > 1:17124906/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:26969233/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaac > 1:23532447/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:1520817/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:32485320/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:30972291/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:35343550/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:30737052/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaa < 1:12768776/67‑1 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTacgaac > 1:8482546/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTaccaac > 1:19641892/1‑68 (MQ=255) gcggcgCTGTTGTAGCCGTTACGCGCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacga < 1:35885055/66‑1 (MQ=255) ggcgCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTacgaacg < 1:22050786/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:10964845/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:31954361/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:15870733/67‑1 (MQ=255) cgcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAcgaacgaa < 1:87179/67‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:6233611/68‑1 (MQ=255) gcTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGa < 1:27500415/68‑1 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGAACGAAGATAATc > 1:34470284/1‑68 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGAAAAt > 1:36023461/1‑67 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTTGTACGAACGAAGATAAt > 1:21370984/1‑67 (MQ=255) tgtAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATc > 1:2323170/1‑68 (MQ=255) aGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgcc > 1:17352729/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTTGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:13742443/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATcgccgc > 1:20213182/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAAATAATcgccgc > 1:5801516/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGCACGAAGATAATagccgc > 1:3679256/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATTATcgccgc > 1:9020557/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:9752276/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:26784431/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:7751654/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:28126037/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:14307604/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc > 1:23934953/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATagccgc > 1:20445603/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTAAGAACGAAGATAAAcgccgc > 1:29240459/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGGACGATCGAAGATTATcgccgc > 1:37998033/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTTGTTCGAACGAAGATAATagccg > 1:19380656/1‑67 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGTACGAACGAAGATAATcgccgc > 1:25822008/1‑68 (MQ=255) ccGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCATTTTTGGTACGAACGAAGATAGTcgccgc > 1:14551987/1‑68 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATcgccgc < 1:1689690/67‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:12436607/68‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:37344106/68‑1 (MQ=255) cGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCa < 1:25633083/68‑1 (MQ=255) ttACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGGTTTTGGGACGAACGAAGATAATAGCCGCATc > 1:22653083/1‑68 (MQ=255) gCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTTCGCACGAAGATAATAGCCGCATCaaaa > 1:13046093/1‑68 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTTGTTCGAACGAAGATAATCGCCCCATCAAAATCtt > 1:33620257/1‑67 (MQ=255) agagCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCtt > 1:6538540/1‑67 (MQ=255) gCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATAAAAATCTTcc > 1:4908293/1‑66 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCt > 1:38594466/1‑67 (MQ=255) ttCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGAAAATCGCCGCAAAAAAAAATTCCGcg > 1:19794835/1‑66 (MQ=255) tCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTc < 1:31548617/68‑1 (MQ=255) cAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTcc < 1:28417532/68‑1 (MQ=255) gCCACTTCCAGAGTCGCGTTTTTTGTACGAACGAAGATAATCGCCGCATCAAAATATTCCGCTTCCAg > 1:35032974/1‑68 (MQ=255) ccACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAgg > 1:9491716/1‑68 (MQ=255) ccACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATAGCCGCATCAAAATCTTCCGCTTCCAgg > 1:7277003/1‑68 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGa < 1:425037/68‑1 (MQ=255) cACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGa < 1:8256736/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:37771437/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:16624380/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:14875381/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:18280978/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:24249403/68‑1 (MQ=255) aCTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGaa < 1:28987243/68‑1 (MQ=255) ttCCAGAGTCGCTTTTTTGGTACGAACGAATATACTCGCCTCCTCAAAATCTTACGATTCAAGGAAAc < 1:3324782/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:36374467/68‑1 (MQ=255) ttCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAAc < 1:3702088/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGt < 1:38924169/68‑1 (MQ=255) ccAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGt < 1:13455952/68‑1 (MQ=255) cAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTa < 1:5424101/68‑1 (MQ=255) agagTCGCGTTTTTTGTACGAACGAAGATAATAGCCGCATCAAAATATTACGCTTCCAGGAAACCTa > 1:11713486/1‑67 (MQ=255) agTCGCGTTTTTGGTACGCACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCCGGAAACGTACCa > 1:21738298/1‑68 (MQ=255) agTCGCGTTTTTGGTACGCACGAAGATAAAAGCCGCATCAAAAACTTCCGCGTCCAGGAAACGTACCa > 1:34665652/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCa > 1:39338786/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTACGCTTCCAGGAAACGTACCa > 1:25055777/1‑68 (MQ=255) agTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATATTACGCGTCCAGGAAACGTACCa > 1:29804042/1‑68 (MQ=255) agTCGCGTTTTTGGTAAGAACGAAGATAATAGCCGCATAAAAATCTTCCGCTTCCAGGAAACGTACCa > 1:21481120/1‑68 (MQ=255) gTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTAcc < 1:20797747/66‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:24698388/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:11830539/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGt < 1:32821069/67‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTg < 1:16335046/68‑1 (MQ=255) cgcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTg < 1:10465805/68‑1 (MQ=255) gcgTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGc < 1:11414608/68‑1 (MQ=255) ttttGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTc < 1:28728013/67‑1 (MQ=255) ttGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGtt < 1:19093968/68‑1 (MQ=255) ggTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGtttt > 1:6824940/1‑68 (MQ=255) | CGTTCAGCGCGGCGCTGTTGTAGCCGTTACGCTCAAGAGCTTCAGCCACTTCCAGAGTCGCGTTTTTGGTACGAACGAAGATAATCGCCGCATCAAAATCTTCCGCTTCCAGGAAACGTACCAGTGCTTCGTTTT > CP009273/3300391‑3300525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |