| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,438 | C→A | 5.0% | A259S (GCA→TCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,438 | 0 | C | A | 5.0% | 1983.6 / 5.0 | 651 | A259S (GCA→TCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (148/440); new base A (14/20); total (163/488) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 653 total aligned reads displayed.
GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCT > CP009273/3462374‑3462505 | cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:12892868/67‑1 (MQ=255) gcgcgAACATAACCCCATGGGTTACTGGGAACGATTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:7548704/68‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTa < 1:19269038/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:21836269/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:16888684/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:32841782/1‑68 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt < 1:28184461/67‑1 (MQ=255) cATGGGTTACTGGACTCGTTTTCTTTCGGATCGCCTGGGCAATATTCCGCGCCTACCCACCAGGCGtt < 1:25278165/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCt < 1:22886706/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:32392574/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:25780621/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:6810662/68‑1 (MQ=255) tGGGTTAATGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:31478924/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt < 1:32223194/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt < 1:34838593/68‑1 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCTTTTTta > 1:33359496/1‑68 (MQ=37) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:17628461/67‑1 (MQ=255) cTGGCATCGTTTTCTTTCGCATCGCCAGGCCAATTTGCCGCGCCTACCCCCCAGGCGTTATTATAAAt > 1:36954860/1‑68 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTTTTATATATg > 1:34539145/1‑68 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTatatat > 1:37069532/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:24715235/1‑67 (MQ=255) tGGCATCGGTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATATATg > 1:18235287/1‑68 (MQ=38) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:14459250/1‑67 (MQ=255) tccttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:27109784/65‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:26674487/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:13161658/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:9432235/68‑1 (MQ=255) cGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGccgccg < 1:22136116/68‑1 (MQ=255) tttttCTTTCGCATCGCGTGGGCAATTTGGCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:13937175/67‑1 (MQ=39) tttttCTTTCGCATCGCCTGGGCAATTTTCAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:28668797/67‑1 (MQ=37) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATCAATGCCGCCGt < 1:36563295/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:8180905/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9761292/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:11499326/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:34240230/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:31058017/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:8456722/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:34990550/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10002656/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:16980596/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17334712/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:38685534/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:19587273/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:37722600/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:23489933/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGCGCAATTTGCCGCGCCTAGCCACCAGGCGTTGTTTTAAATGCCGCCGt < 1:1210349/68‑1 (MQ=255) gTTTTCTTTCGCACCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:12488568/68‑1 (MQ=255) gTTTTCTATCGCATCGCGTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:18991298/68‑1 (MQ=38) gTTTTCTATCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10628616/68‑1 (MQ=255) gTTTTCTATCGCATCCCCTGGGCAATTTGCCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:15090260/68‑1 (MQ=38) ttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:25957835/67‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:11689030/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:6066301/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:29562456/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:17792203/68‑1 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:27643540/68‑1 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:20275963/68‑1 (MQ=255) ttggCAACGCCTGTGCAATATGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:32573898/65‑1 (MQ=38) ttcTCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:39529693/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:34003123/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:28932110/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:39064402/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:3620506/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:35776324/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:6409333/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:31706718/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:20961147/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:30744390/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:19429526/68‑1 (MQ=255) atcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:5659617/67‑1 (MQ=255) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:16102215/68‑1 (MQ=255) accGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:32070860/66‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:35507080/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:8978218/68‑1 (MQ=255) gtGGGCAATTTTAAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:4238023/67‑1 (MQ=37) gtGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:23396932/67‑1 (MQ=255) cTGGGCAATTTTTAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:37860947/68‑1 (MQ=37) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:14776896/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:15551489/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:8717131/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:10993564/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:22503534/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:30907471/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:23737098/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:30222343/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:2625213/68‑1 (MQ=255) gggCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGt < 1:25138821/68‑1 (MQ=255) gCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCGGTTATAGATAACGTACCATGTa < 1:2978514/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:21325616/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:37309231/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:5231984/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:39398044/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:2045675/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:7315356/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTGATAAATGCCGCCGTGTGAGATAACGTACCATgag > 1:36710059/1‑65 (MQ=255) gCAATTTGCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:38261323/67‑1 (MQ=255) cAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:24276545/67‑1 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga > 1:13452265/1‑68 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga > 1:38079156/1‑68 (MQ=255) aaTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga < 1:10350129/67‑1 (MQ=255) | GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCT > CP009273/3462374‑3462505 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |