| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,063,837 | A→T | 7.0% | intergenic (‑76/+38) | yihR ← / ← yihS | mutarotase superfamily protein, YihR family/aldose‑ketose isomerase; D‑mannose isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,063,837 | 0 | A | T | 7.0% | 1409.6 / 9.4 | 491 | intergenic (‑76/+38) | yihR/yihS | mutarotase superfamily protein, YihR family/aldose‑ketose isomerase; D‑mannose isomerase |
| Reads supporting (aligned to +/- strand): ref base A (351/68); new base T (2/38); total (353/138) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.77e-25 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 499 total aligned reads displayed.
CCGTGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCC > CP009273/4063770‑4063902 | ccGTGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTa > 1:34915462/1‑68 (MQ=255) cGTGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAg < 1:26877960/68‑1 (MQ=255) gggAGGCTGATGTCACACCCAAAACCTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAgg > 1:24883142/3‑68 (MQ=25) gTGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAgg > 1:3887086/1‑68 (MQ=255) gTGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAgg > 1:17667807/1‑68 (MQ=255) tGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAggg > 1:14772611/1‑68 (MQ=255) gAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGCGGGTTAGGga > 1:5875036/1‑68 (MQ=255) gAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAggg < 1:32595356/67‑1 (MQ=255) gAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAggg < 1:39605235/67‑1 (MQ=255) gAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAggg < 1:7175017/67‑1 (MQ=255) gAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGga > 1:20625461/1‑68 (MQ=255) gAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGga > 1:12317794/1‑68 (MQ=255) aGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGgag > 1:9868578/1‑68 (MQ=255) ggCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGgaga > 1:14193505/1‑68 (MQ=255) ggCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGgaga > 1:7488397/1‑68 (MQ=255) ggCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGgaga > 1:36165263/1‑68 (MQ=255) ggCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGAAGAGGGTTAGGgaga > 1:6606809/1‑68 (MQ=255) ggCTGATGTC‑AACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGagag > 1:18062106/1‑68 (MQ=255) gCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGagag > 1:38099923/1‑68 (MQ=255) cTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAgg > 1:23880267/1‑68 (MQ=255) tGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAggg > 1:21344834/1‑68 (MQ=255) gATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAgggg > 1:3776340/1‑68 (MQ=255) gTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGaaa > 1:12680506/1‑68 (MQ=255) gTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGaaa > 1:1426748/1‑68 (MQ=255) aaCCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAAccc > 1:540557/1‑68 (MQ=255) aaCCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAAccc > 1:25356504/1‑68 (MQ=255) aaCCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAAccc > 1:20225341/1‑68 (MQ=255) aCCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAAccc > 1:29702224/1‑67 (MQ=255) aCCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAAccc > 1:33322631/1‑67 (MQ=255) cccAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGc > 1:31523453/1‑68 (MQ=255) aaaaCTCCC‑CCGCACGGGCCCCGCCCCCGTTT‑GGGGCGTGGGTTTGGGTGCGGGCAAAACCCGCAccc < 1:18860282/68‑1 (MQ=255) aaaCTGCCCCCGGACGGCCCCCGCCCCCCTTG‑GGGGTGTGGTTTCGGGCGCGGGGAAAACCCGCAccc < 1:35566118/68‑1 (MQ=255) aCTGCC‑CCGGACGGACCCCTCGCCCCTTT‑GGGGAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTAt < 1:15579519/68‑1 (MQ=21) aCTGCA‑CCGGCGGGTCCCCTCGCCCCTTT‑GGGTTTTGGGTTAGGGACCGGGGAAAACCCGCACCCTAt < 1:33983330/68‑1 (MQ=21) aCTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAt > 1:11264796/1‑68 (MQ=255) aCTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAt > 1:18537059/1‑68 (MQ=255) gccA‑CCGGCCGGCCCCCGCGCCCGTTT‑GGGGAGTGGGTTCGGGAGCGGGGAAAACCCGCACCCTAttt < 1:22561329/66‑1 (MQ=25) gCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTAtttt > 1:32391466/1‑68 (MQ=255) a‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGTGGGGAAACCCCGCACCCTATTTTCa > 1:12975957/1‑68 (MQ=255) aCGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa > 1:27842302/1‑68 (MQ=255) aCGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa > 1:33069676/1‑68 (MQ=255) aCGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCa > 1:17060079/1‑68 (MQ=255) aCGGTCCCCTCGCCCCTGT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCa > 1:27245572/1‑68 (MQ=255) cGGCCCCCGCGCCCGTTG‑GGGGTTTGGGTTTGGGTGCGGGGAAAACCCGCACCCTATTTTCAGATCa < 1:1358905/67‑1 (MQ=255) gTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGtt > 1:23302258/1‑68 (MQ=255) ccccTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAt > 1:21825696/1‑68 (MQ=255) ccccTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAt > 1:35320202/1‑68 (MQ=255) cccTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTAtt > 1:29127160/1‑68 (MQ=255) cTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTc > 1:20864426/1‑68 (MQ=255) cGCCCGTTT‑GGGGAGAGGGTTTGGGAGAGGGGAAAACCCGCAGGCTATTTTCAGATCAGTTATTTCGc > 1:11649289/1‑68 (MQ=37) cGCCCCTTT‑GGGGGGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc > 1:28591798/1‑68 (MQ=255) cGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTATTTCGc > 1:8280298/1‑68 (MQ=255) cGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCCCCCTATTTTCAGATCAGTTATTTCGc > 1:11485082/1‑68 (MQ=255) cGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc > 1:19816610/1‑68 (MQ=255) cGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc > 1:16010320/1‑68 (MQ=255) cGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc > 1:1445702/1‑68 (MQ=255) cGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGc > 1:33590915/1‑68 (MQ=255) gCCCGTTT‑GGGGTGTGGGTTAGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa < 1:4716276/68‑1 (MQ=255) gCCCGTTT‑GGGCTGAGGTTTTGGGACCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa < 1:36346995/68‑1 (MQ=255) gCCCCTTT‑GGGGAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa < 1:15441151/68‑1 (MQ=255) ccccgTTG‑GGGGCGTGGTTTGGGGCGCGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCa < 1:31993379/67‑1 (MQ=255) ccccTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAt > 1:31137982/1‑68 (MQ=255) ccccTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAt > 1:12049389/1‑68 (MQ=255) cccTTT‑GGGGAGTGGGTTCGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAtt < 1:25963982/68‑1 (MQ=39) cccTTT‑GGGGAGTGGGTTCGGCACTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAtt < 1:1058005/68‑1 (MQ=255) ccTTTGGGGGTGTGGTTTTGGGTGCGGGCAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCAtt < 1:13337499/68‑1 (MQ=255) ccTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa > 1:19359468/1‑68 (MQ=255) ccTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa > 1:6766/1‑68 (MQ=255) ccTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTa > 1:2889878/1‑68 (MQ=255) ttt‑GGGGTGTGGTTTTGGGAGTGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat < 1:24479637/68‑1 (MQ=255) ttgggggTGTGGTTTCGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat < 1:25060523/65‑1 (MQ=255) ttgggggAGTGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat < 1:35744401/65‑1 (MQ=255) t‑GGGGAGTGGGTTTGGGAGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAatat < 1:30619158/68‑1 (MQ=255) gggggAGTGGTTTTGTGCGCGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAatat < 1:26654954/67‑1 (MQ=255) ggggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAatat > 1:3907270/1‑67 (MQ=255) ggggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATc > 1:10780530/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:7767506/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:10033406/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:16604424/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:30939157/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:18440528/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:5710399/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:4953857/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:21046329/1‑68 (MQ=255) ggAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:22122103/1‑68 (MQ=255) gagaGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:23030825/1‑67 (MQ=255) gagaGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCca > 1:5981103/1‑67 (MQ=255) agagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 1:19071745/1‑68 (MQ=255) agagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 1:37324892/1‑68 (MQ=255) agagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 1:3685770/1‑68 (MQ=255) agagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 1:28263276/1‑68 (MQ=255) agagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 1:34450980/1‑68 (MQ=255) agagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagc > 1:8389747/1‑68 (MQ=255) agagGGTTAGGGAGAGGGG‑AAACCCGCACCCTATTTTAAGATCAGTTATTTCGCATTAATATCcagca > 1:4322069/1‑68 (MQ=39) gagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagca > 1:29515838/1‑68 (MQ=255) gagGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagca > 1:9723111/1‑68 (MQ=255) gggTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCcagcag > 1:30027716/1‑67 (MQ=255) gTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAc > 1:9133284/1‑67 (MQ=255) ttAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAat < 1:8850476/55‑1 (MQ=255) ttAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGAccc > 1:16387638/1‑68 (MQ=255) | CCGTGAGGCTGATGTCAAACCCAAAAACTGCA‑CCGGACGGTCCCCTCGCCCCTTT‑GGGGAGAGGGTTAGGGAGAGGGGAAAACCCGCACCCTATTTTCAGATCAGTTATTTCGCATTAATATCCAGCAGACCC > CP009273/4063770‑4063902 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |