BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,043	T→A	6.9%	noncoding (30/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,043	0	T	A	6.9%	1072.8 / 19.2	347	noncoding (30/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (221/98); new base A (14/14); total (235/112)
Fisher's exact test for biased strand distribution p-value = 5.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

AGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691977‑692110

 ggTAAGCGTCTTGAATAAGTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:35299551/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:4410019/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:4704076/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:20491323/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:6847274/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:28829222/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:19568650/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:3249972/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:14199768/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:5690548/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:36145326/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:33891961/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:18991154/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:30632433/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:23899580/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAAAcc                                                                   >  1:16314597/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAAcc                                                                   >  1:378156/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAAcc                                                                   >  1:33593068/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCAGGAATAAGAATcc                                                                   >  1:19879447/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCACAATcc                                                                   >  1:39497237/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGCGTACGAGGATTCGAACCTCGGAATGCAGGAATCAGAATcc                                                                   >  1:19247449/1‑68 (MQ=21)

   tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:7510755/1‑68 (MQ=255)

   tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:37359081/1‑68 (MQ=255)

   tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:26322625/1‑68 (MQ=255)

     aGCGTCTTGAGTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:23755585/1‑67 (MQ=255)

     aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:16107699/1‑67 (MQ=255)

     aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:29043869/1‑67 (MQ=255)

     aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                                >  1:14430341/1‑67 (MQ=255)

      gcttCTTTAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:37107367/65‑1 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCTGtc                                                               >  1:1769973/1‑65 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:28448196/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:29873395/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:29726017/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:4067910/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:8702130/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:15397903/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:7286965/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:7654967/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAATCCGGTg                                                               >  1:21066327/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAAACCTGtc                                                               >  1:24865175/1‑65 (MQ=17)

        gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                              <  1:23133477/66‑1 (MQ=255)

        gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGc                                                              <  1:18218529/66‑1 (MQ=11)

         tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:31749604/1‑68 (MQ=255)

            tttaTAATTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:17300461/65‑1 (MQ=0)

            tttaTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:35652029/65‑1 (MQ=255)

               aTAAATTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCt                                                     <  1:15017538/68‑1 (MQ=11)

               aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:16537686/1‑68 (MQ=40)

                 aaaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                   >  1:3124380/1‑68 (MQ=37)

                 aaaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                   >  1:36579933/1‑68 (MQ=37)

                  aaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTgg                                                  <  1:12566568/68‑1 (MQ=255)

                   tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGc                                                 <  1:10722257/67‑1 (MQ=11)

                   tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:21579656/67‑1 (MQ=14)

                   tttGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCATAATCCGGTGCCTTACCGCTTGGc                                                 <  1:36851240/67‑1 (MQ=11)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:12011498/1‑68 (MQ=14)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAATCCGGTGCCTTACCGCCTgcct                                                >  1:19110421/1‑65 (MQ=11)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:8530812/1‑68 (MQ=31)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:33350195/1‑68 (MQ=31)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATAAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:26918121/1‑68 (MQ=14)

                               aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                                     <  1:23682382/68‑1 (MQ=31)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:25196658/1‑68 (MQ=37)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:38685675/1‑68 (MQ=37)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:8223283/1‑68 (MQ=37)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:33748433/1‑68 (MQ=37)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:22728706/1‑68 (MQ=37)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:5404595/1‑68 (MQ=37)

                                   ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAtt                                 >  1:22838488/1‑68 (MQ=39)

                                    gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                                >  1:20610103/1‑68 (MQ=40)

                                      ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                               <  1:8046785/67‑1 (MQ=255)

                                      ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                               <  1:38247353/67‑1 (MQ=255)

                                      ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:3842852/68‑1 (MQ=255)

                                      ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:25867599/68‑1 (MQ=255)

                                      ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:19440113/68‑1 (MQ=255)

                                      tgcGAACCTCGGAATGCCGGAAGCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTgg                               <  1:5926082/65‑1 (MQ=255)

                                         gAACCTCGGAATGCCGGAATCAGAATCCTGTGACTTACCCCATTGAGATACCCCAACAAATTGGtttt                           >  1:11843680/1‑68 (MQ=17)

                                          aaCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTg                          >  1:9775970/1‑68 (MQ=255)

                                           aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGCttttta                         >  1:18537751/1‑66 (MQ=255)

                                           aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTGACAGCTTTGCGAAACACCCAGAAATGGGGTTTGa                         >  1:9282474/1‑68 (MQ=2)

                                               cGGAATGCCGGAATCAGATTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt                     <  1:10481282/68‑1 (MQ=255)

                                               cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt                     <  1:18343376/68‑1 (MQ=255)

                                                ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGGTTTGAAttt                     >  1:3197098/1‑67 (MQ=14)

                                                  aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:168409/67‑1 (MQ=255)

                                                   aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg                 >  1:34217647/1‑68 (MQ=255)

                                                    tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:9035436/68‑1 (MQ=255)

                                                     gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:10107970/68‑1 (MQ=255)

                                                        ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:22489784/68‑1 (MQ=255)

                                                        ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:21866468/68‑1 (MQ=255)

                                                        ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:32796362/68‑1 (MQ=255)

                                                        ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:1135596/68‑1 (MQ=255)

                                                          aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:2007034/67‑1 (MQ=255)

                                                           ttCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:15775013/66‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:24549467/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:7065375/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:2768982/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:1513351/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:12206507/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:3471599/67‑1 (MQ=255)

                                                            tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc        >  1:5088101/1‑68 (MQ=255)

                                                            tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc        >  1:15807924/1‑68 (MQ=255)

                                                                 aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATa    >  1:6529022/1‑67 (MQ=255)

                                                                  tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  <  1:13594586/68‑1 (MQ=255)

AGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691977‑692110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`