| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,181 | T→C | 5.3% | D43D (GAT→GAC) | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,181 | 0 | T | C | 5.3% | 2146.5 / 65.1 | 601 | D43D (GAT→GAC) | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (309/258); new base C (22/10); total (333/268) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
Only 100 of 608 total aligned reads displayed.
TACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAG > CP009273/4264114‑4264248 | gCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATaaa > 1:2248395/1‑68 (MQ=255) gCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATaaa > 1:17181284/1‑68 (MQ=255) gCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATaaa > 1:10958547/1‑68 (MQ=255) gCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGAGaaa > 1:18330414/1‑68 (MQ=255) tACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGc > 1:1559674/1‑68 (MQ=255) aCATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCg > 1:20940919/1‑68 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:39509157/1‑67 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:24271584/1‑67 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:15178732/1‑67 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:28568959/1‑67 (MQ=255) ccAAATGGTGGCGCAGTTACCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:27860055/1‑67 (MQ=255) cAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCgg < 1:1931634/67‑1 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:22930889/1‑68 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:23197023/1‑68 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:23766016/1‑68 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:25482497/1‑68 (MQ=255) aaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCga < 1:36458588/68‑1 (MQ=255) aTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgag < 1:4389610/68‑1 (MQ=255) aTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgag > 1:31738034/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:9591661/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:32195816/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:32754415/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga < 1:3336862/68‑1 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:21980547/1‑68 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:32405502/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:26257038/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:19499231/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:19916321/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:13570760/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:22137225/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:16880758/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:8944853/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGa < 1:38860280/68‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:34790226/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:16103367/67‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:4183329/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:37320614/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:27449720/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:20678180/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:16243843/68‑1 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:29864249/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:28111572/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:7428330/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGAATGaa > 1:17823267/1‑68 (MQ=255) aTTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATg > 1:29517767/1‑68 (MQ=255) aTTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATg > 1:25019740/1‑68 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGCGATGAAAGAACAGACTGAATGGc < 1:5022056/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:5197201/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:72072/68‑1 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:15811337/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:22284550/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:1151942/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:13783529/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:34960628/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:6672988/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:35269315/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:13275816/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:38204684/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:29321980/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:25836654/1‑68 (MQ=255) gCTGGCTACTTCCGAATCATGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:4006943/1‑68 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGGTAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:26600610/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:8725799/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:7750946/1‑67 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:34409498/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:39111412/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:12999671/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:12108898/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:5395130/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:30649473/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:28853430/68‑1 (MQ=255) tACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGtt < 1:620189/67‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:18640835/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:9377131/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:37649123/68‑1 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:35568303/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:31318927/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCt < 1:20127233/68‑1 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCt < 1:10445079/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:7980017/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:21576722/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:20776007/68‑1 (MQ=255) aTCCTTGCGTGATAAAGCGACCGGCGATATGAAAGAACAGACTGAATGGAACCGCGTTGTGCTGTTCg > 1:30307426/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:30912126/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:10236313/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:12548441/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:4801264/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:33673883/1‑68 (MQ=255) tCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:14228075/1‑67 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:11946437/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:10078067/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:21267713/2‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:26837144/1‑68 (MQ=255) cGTTATAAAGCGACCGGCGAGATGAACCAACATACTGAATGGAAAAGCGTTGTGCTGTTCGGCAAACt < 1:3701552/68‑1 (MQ=255) cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCaa > 1:30159347/1‑65 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:14679753/68‑1 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:18832458/68‑1 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:35796866/68‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:17426523/67‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:24584936/67‑1 (MQ=255) | TACGCTACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAG > CP009273/4264114‑4264248 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |