| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,185 | G→C | 5.4% | A45P (GCG→CCG) ‡ | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,185 | 0 | G | C | 5.4% | 2120.5 / 80.9 | 589 | A45P (GCG→CCG) ‡ | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (294/263); new base C (22/10); total (316/273) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01 | |||||||||||
Only 100 of 600 total aligned reads displayed.
TACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGT > CP009273/4264119‑4264252 | tACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGc < 1:18698016/68‑1 (MQ=255) aCATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCg > 1:11991341/1‑68 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:11631891/1‑67 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:39509157/1‑67 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:16502393/1‑67 (MQ=255) cAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCgg < 1:22897044/67‑1 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:13013365/1‑68 (MQ=255) aTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgag > 1:27596945/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:32195816/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:32754415/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga < 1:3336862/68‑1 (MQ=255) ggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGAt > 1:12166247/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:39314784/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:17504521/1‑68 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:20779772/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:25661915/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:32405502/67‑1 (MQ=255) ggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa < 1:19519549/68‑1 (MQ=255) ggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa > 1:27219856/1‑68 (MQ=255) gcgcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa < 1:20330645/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:15833883/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGa > 1:38620603/1‑68 (MQ=255) cAGTTGCCAACATTACGCTGGCTACTTACGAATCCTGGCGTGATATAGCGACCGGCGAGATGAAAGaa < 1:19925193/68‑1 (MQ=255) aGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAAc > 1:37429647/1‑68 (MQ=255) aGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAAc > 1:20697663/1‑68 (MQ=255) aGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAAc > 1:13675886/1‑68 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:16916219/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:13321311/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGa < 1:14783848/68‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGa < 1:2358398/68‑1 (MQ=255) ccAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg > 1:6177100/1‑65 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACt < 1:15691488/67‑1 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACt < 1:8107808/67‑1 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTg > 1:37222847/1‑68 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:35558852/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:10247858/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:31732302/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:6433443/68‑1 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:29236712/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:2832752/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:7428330/1‑68 (MQ=255) aTTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATg > 1:26344063/1‑68 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGCGATGAAAGAACAGACTGAATGGc < 1:5022056/68‑1 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:17325328/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:21030707/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:4405805/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:22397958/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:34960628/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:35269315/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:8740906/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:8980834/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:22088606/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:25403906/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:7121466/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:9444169/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:37843650/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:5151179/1‑68 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:29385484/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:7750946/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:10545012/1‑67 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:11001690/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:34409498/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:30625252/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:30131060/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:28705492/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:13870915/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:1433833/68‑1 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:24104619/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:16109189/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:31318927/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCt < 1:19373263/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:21360490/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:25003106/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:21721735/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt > 1:30290558/1‑68 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:17849076/68‑1 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:4801264/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:4199158/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:33673883/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:4041357/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:30912126/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGGTGTGCTGTTc < 1:15475318/67‑1 (MQ=255) agcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCgg < 1:9607880/66‑1 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:38864747/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:10078067/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:29848451/2‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:36830940/1‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:26699917/1‑68 (MQ=255) cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCaa > 1:22263136/1‑65 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc > 1:34774578/1‑65 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc < 1:12701015/67‑3 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc < 1:27994499/67‑3 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:35796866/68‑1 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg > 1:23062370/1‑68 (MQ=255) gTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCt < 1:26002165/67‑1 (MQ=255) aTAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCa > 1:18972905/1‑68 (MQ=255) tAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAg > 1:18574536/1‑68 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg > 1:2452551/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGGAAACTGGCAGAAGt > 1:36425123/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:5514144/1‑68 (MQ=255) | TACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGT > CP009273/4264119‑4264252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |