| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,186 | C→A | 5.5% | A45E (GCG→GAG) ‡ | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,186 | 0 | C | A | 5.5% | 2128.7 / 71.3 | 592 | A45E (GCG→GAG) ‡ | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (297/262); new base A (23/10); total (320/272) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.29e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 613 total aligned reads displayed.
TACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTG > CP009273/4264119‑4264253 | aCATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCg > 1:11991341/1‑68 (MQ=255) aCATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCg > 1:17078618/1‑68 (MQ=255) ccAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCg > 1:11631891/1‑67 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:23766016/1‑68 (MQ=255) aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:22930889/1‑68 (MQ=255) aTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgag > 1:31738034/1‑68 (MQ=255) aTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgag > 1:27596945/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:9591661/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga < 1:3336862/68‑1 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:32754415/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:32463252/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:26706860/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:22473053/1‑68 (MQ=255) ggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGAt > 1:12166247/1‑68 (MQ=255) ggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGAt > 1:13126106/1‑68 (MQ=255) ggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGAt > 1:14076055/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:39314784/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:32208888/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:28649930/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:21980547/1‑68 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:5051534/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:20877014/67‑1 (MQ=255) ggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa > 1:27219856/1‑68 (MQ=255) ggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa > 1:21048690/1‑68 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:8944853/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:25413066/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:22137225/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGa < 1:15555756/68‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGa > 1:38620603/1‑68 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:20724048/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:19648052/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:34790226/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGa < 1:2500967/68‑1 (MQ=255) ccAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg > 1:6177100/1‑65 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACt < 1:8107808/67‑1 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACt < 1:17349223/67‑1 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTg > 1:37222847/1‑68 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:26046017/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:4818816/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:6433443/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:23208137/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:4441417/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:3013834/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:10247858/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:14852642/68‑1 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:29236712/1‑68 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:16706287/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:5197201/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:19959670/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:19426916/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCCAGATGAAAGAACAGACTGAATGGc < 1:28324822/68‑1 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:24434683/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:13754062/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:7121466/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:16203939/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:22284550/1‑68 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:8725799/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:10545012/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:7750946/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:29385484/1‑67 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:30625252/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:20346903/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:34409498/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:18714531/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:11001690/68‑1 (MQ=255) cTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGtt > 1:39518520/1‑68 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:9377131/68‑1 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:31318927/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:35568303/1‑65 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:21576722/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt > 1:30290558/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:4199158/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:33673883/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:30912126/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:35097760/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:4041357/1‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:38864747/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:10078067/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:36431113/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:17632634/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:29848451/2‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:37439161/1‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:36830940/1‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:14487468/1‑68 (MQ=255) cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAAc < 1:13358568/67‑1 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc > 1:15704166/1‑65 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc > 1:7443300/1‑65 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc < 1:12701015/67‑3 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc < 1:27994499/67‑3 (MQ=255) gTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCt < 1:35285408/67‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:24584936/67‑1 (MQ=255) tAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAg > 1:18574536/1‑68 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg > 1:25715445/1‑68 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg < 1:37887921/68‑1 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:18990744/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:35951238/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:15974354/1‑68 (MQ=255) cGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTg > 1:2654307/1‑68 (MQ=255) cGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTg > 1:5646989/1‑68 (MQ=255) cGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTg > 1:17874336/1‑68 (MQ=255) | TACATGCCAAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTG > CP009273/4264119‑4264253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |