| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,280 | T→G | 8.9% | S76S (TCT→TCG) ‡ | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,280 | 0 | T | G | 8.9% | 2634.9 / 185.5 | 778 | S76S (TCT→TCG) ‡ | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (227/481); new base G (30/39); total (257/521) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.08e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01 | |||||||||||
Only 100 of 811 total aligned reads displayed.
ATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTACA > CP009273/4264214‑4264347 | aTGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTtctc > 1:20106125/1‑68 (MQ=255)aTGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTtctc > 1:6927534/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:5680956/1‑68 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:21973564/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:29622864/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:14988736/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:18165543/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:18852924/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:7165583/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:11439438/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:7452903/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:26215307/67‑1 (MQ=255) cACCGCGTTGGGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:37278535/67‑1 (MQ=255) atcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGt < 1:28131335/65‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:3350318/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:34071976/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:1713869/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:19270523/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:34676582/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt < 1:15711276/68‑1 (MQ=255) cgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCtaca > 1:3954431/1‑66 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTtata > 1:36107887/1‑67 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTtata < 1:4930872/67‑1 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:16424686/1‑68 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:34919069/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGGGAATATCTGCGTAAAGGTTATCAGGTTTATATCGAAg > 1:28459960/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:10246792/1‑68 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:17533649/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:38959895/67‑1 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:2773895/67‑1 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:18538660/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:37682510/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATATGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:1049632/1‑67 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:16917599/68‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:23992734/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:9382417/68‑1 (MQ=255) aaaCTGGCAGAAGTGGCGCGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGTTGCg > 1:30733172/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:8099115/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:38484304/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:13360573/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:24808677/1‑68 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:7025069/1‑68 (MQ=255) tGGCAGAAGTGTCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:12549963/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATTTCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:26537274/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:6341505/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:16187787/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:26955533/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:4607211/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:5329226/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTGCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:3180788/68‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGt < 1:29942099/66‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa < 1:6744971/67‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa < 1:10709426/67‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa < 1:14363507/67‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa > 1:24194420/2‑68 (MQ=255) aaGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa > 1:315346/1‑66 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:12811571/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:5479940/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:13946103/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:33899324/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:22976663/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:2337899/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:24594632/65‑1 (MQ=255) aGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagc < 1:35593970/67‑3 (MQ=255) gTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATg > 1:15248077/1‑68 (MQ=255) tGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATgg > 1:29301712/1‑68 (MQ=255) gcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa > 1:2252561/1‑67 (MQ=255) gcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa < 1:29040792/67‑1 (MQ=255) gcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa < 1:24421459/67‑1 (MQ=255) gcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGGCAGCTGCGTACCCGTAAATGGACCGa < 1:5879096/67‑1 (MQ=255) gaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCa < 1:32220855/68‑1 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCaa < 1:30275488/67‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:21118120/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:10040530/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:2737423/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:2160500/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:22222087/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:11896167/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:7865478/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:8667750/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:17900329/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:3450878/68‑1 (MQ=255) tGCGTAAAGTTTCTAAGGTTTATGTCGAAGGTCTGCTGCGTACCAGTAAATGGACCGATCAATGCgg > 1:20607543/1‑67 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:14715166/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:3816317/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:883005/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:19717117/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:32589436/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:3685784/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:25525828/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:25926863/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:14153647/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:20771706/68‑1 (MQ=255) ggTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc > 1:25273300/1‑67 (MQ=255) ggTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc > 1:31021912/1‑67 (MQ=255) tctcAGGTTTATATCGAAGGGCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCt < 1:4319427/67‑1 (MQ=255) ctcAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTac < 1:33105168/68‑1 (MQ=255) tcAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTaca < 1:12299732/68‑1 (MQ=255) tcAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTaca < 1:23129644/68‑1 (MQ=255) tcAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTac > 1:19427585/1‑67 (MQ=255) | ATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATCGCTACA > CP009273/4264214‑4264347 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |