| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 999,598 | A→C | 8.9% | T66P (ACG→CCG) | ycbF → | putative periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 999,598 | 0 | A | C | 8.9% | 1467.9 / 22.9 | 563 | T66P (ACG→CCG) | ycbF | putative periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base A (357/147); new base C (5/53); total (362/201) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.07e-21 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 573 total aligned reads displayed.
GATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCT > CP009273/999531‑999665 | tatCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAc > 1:6178503/1‑67 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAAGAGTAAAATCAACCGCCCAAcgc < 1:18415229/68‑1 (MQ=255) atCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgc < 1:14948200/68‑1 (MQ=255) tCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAAcgcg < 1:32620761/68‑1 (MQ=255) ttttACTGCCTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg < 1:33286502/68‑1 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg > 1:16460259/1‑68 (MQ=255) ttttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTgg > 1:27858271/1‑68 (MQ=255) tttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTggg < 1:24046588/68‑1 (MQ=255) ttACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTggg > 1:4870958/1‑67 (MQ=255) tACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGcc > 1:29122527/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:32469379/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:30529943/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:23597075/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:8692019/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:14411964/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:25850762/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:16172002/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:7016761/1‑68 (MQ=255) aCTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCg > 1:12143566/1‑68 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTTGGGCCggg < 1:7698897/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:17935688/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg < 1:11319156/68‑1 (MQ=255) tGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCggg > 1:12674634/1‑68 (MQ=255) cTAATACCAGTCAGGAATCCTGGCTTATTTACAGTAAAATCAACCGCCCAACGCTTTGTGCCgggggg > 1:32100477/1‑68 (MQ=255) tACCTGTCAGTAATCCTTTCTTATTAACAGTAAAAACAACCGCCCCACGCGTTGGGCCGGGGGGGAAg < 1:12904379/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:24175484/68‑1 (MQ=255) tACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAg < 1:31239280/68‑1 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:26864158/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:27024521/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:34591691/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:417646/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:24490434/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:10132387/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:29308340/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:4351131/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:33690564/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:22598486/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:22069459/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:21430451/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:17053127/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:18599680/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:5567383/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:20106526/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:18917883/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:7210952/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGc > 1:9019365/1‑68 (MQ=255) aCCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTCCGCCGGGGGGGAAGc > 1:18251970/1‑68 (MQ=255) cAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCg > 1:31785149/1‑67 (MQ=255) cAGTCAGGAACCCTGGCTTATAAACAGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGt < 1:6654919/68‑1 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:10982447/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:27551949/1‑67 (MQ=255) tCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCg > 1:20756014/1‑67 (MQ=255) gcAGGAATCCTCGCTTATTAACAGAAAAATCAACCGACCAACGCGTGGGGCCGGGGGGGAAGCGGCGa < 1:22431076/67‑1 (MQ=255) cAGTAATCCTGGCTTATTAACAGTAAAATCAACCGCCCACCGCGTGGGGCCGGGGGGGAAGCGTCg < 1:922557/66‑1 (MQ=255) aaTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGt > 1:5443222/1‑67 (MQ=255) ttCCTTGATTATAAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:23278440/66‑1 (MQ=255) aTCCTGGCTTATTACCTGTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:15505325/67‑1 (MQ=255) aTCCTGGCTTATTAACATTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:33999773/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:24746471/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:14042399/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:30835151/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTg > 1:6510303/1‑67 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:9557697/67‑1 (MQ=255) aTCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTGGGGCCGGGGGGGAAGCGTCGACAGTg < 1:19021664/67‑1 (MQ=255) aTCCTGGATTATAAACATAAAAATCAACCCCCCAAGGGTGTGGGCCGGGGGGGAAGCGTCGACAGTg < 1:13324176/67‑1 (MQ=255) ggCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:29907086/68‑1 (MQ=255) ggCTTATTAACAGTAAAAACAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:26536136/68‑1 (MQ=255) ggCTTATAAACATTAAAATCAACCGCCCCACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:2882015/68‑1 (MQ=255) ggCTTATAAACATTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:30144873/68‑1 (MQ=255) cTTATTAANAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:5842207/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:3797249/1‑68 (MQ=255) cTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc > 1:752139/1‑68 (MQ=255) cTTATTAACACTAAAATCAACCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCa < 1:10458354/66‑1 (MQ=255) ttattaACAGTAAAATCACCCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:19681388/67‑1 (MQ=255) ttattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCa < 1:17371830/68‑1 (MQ=255) ttataaACAGTAAAATCCACCCCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCAcc < 1:8216879/67‑1 (MQ=255) tattaACAGTAAAATCACCCCCCCACCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:19202913/68‑1 (MQ=255) tattaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:16925556/68‑1 (MQ=255) tattaACAGTAAAATCAACCCCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:29654693/68‑1 (MQ=255) tataaACATTAAAATCAACCGCCCAACGGTTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAt < 1:20305427/68‑1 (MQ=255) attaACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt < 1:15136175/68‑1 (MQ=255) taACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:9786605/1‑66 (MQ=255) aaCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCAtt > 1:16715133/1‑65 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACGGTGCCAGCCCCATTACTg > 1:20964253/1‑68 (MQ=255) aCAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTg > 1:15988236/1‑68 (MQ=255) tAAAATCAACCGCCCAACGCGTTGGGCAGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGcc > 1:25120885/1‑67 (MQ=255) aaaaTCACCCGCCCACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGcc < 1:12787863/66‑1 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:2386809/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:19508867/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:3538358/1‑68 (MQ=255) aTCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTcc > 1:32987745/1‑68 (MQ=255) aCCGCCCACCGCGCTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCa < 1:11700354/68‑1 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGcc > 1:7962365/1‑67 (MQ=255) aCCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCACCACCATTACTGTCCGCTCCGcc > 1:27285002/1‑67 (MQ=255) ccGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCAc < 1:28278898/68‑1 (MQ=255) cGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACt > 1:21687620/1‑68 (MQ=255) gCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCCCCAATACAGGCCCCTCCGCCAcat > 1:14109148/1‑66 (MQ=255) ccccACCGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACtt < 1:26694956/67‑1 (MQ=255) ccAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTAt > 1:5076317/1‑68 (MQ=255) aCGCGTGGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATtct < 1:13731269/68‑1 (MQ=255) | GATATCTATTTTACTGACTAATACCAGTCAGGAATCCTGGCTTATTAACAGTAAAATCAACCGCCCAACGCGTTGGGCCGGGGGGGAAGCGTCGACAGTGCCAGCACCATTACTGGCCGCTCCGCCACTTATTCT > CP009273/999531‑999665 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |