| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,711,673 | A→C | 8.6% | V72G (GTT→GGT) | yfiF ← | putative methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,711,673 | 0 | A | C | 8.6% | 804.7 / 8.0 | 288 | V72G (GTT→GGT) | yfiF | putative methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (201/57); new base C (3/26); total (205/83) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 299 total aligned reads displayed.
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 | aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:5189055/68‑1 (MQ=255)aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:20943406/68‑1 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g > 1:16459166/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g > 1:15901363/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g > 1:16913286/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g > 1:18899797/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g > 1:12355933/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑g > 1:9451865/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑Gt < 1:2349427/68‑1 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑Gt > 1:32909201/1‑67 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑Gt > 1:10257319/1‑67 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑Gt > 1:802884/1‑67 (MQ=255) aCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGcc < 1:26713317/68‑1 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCAc > 1:1216390/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCAc > 1:32131768/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCAc > 1:33714002/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCAc > 1:1258916/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCAc > 1:30750063/1‑68 (MQ=255) aCTGATGCCACCGGGATCGGCCTTTACCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGccggg < 1:15644649/68‑4 (MQ=255) cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACg > 1:3299832/1‑67 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGaa > 1:24541790/1‑68 (MQ=255) tGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGaa > 1:11554574/1‑68 (MQ=255) tGCCACCGTGATCGGCCATTTCCGGCGTCTCATCCCCCGGCGCGCCGGAAACC‑GTGCGCCACGGCGaa > 1:10146895/1‑68 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGaa > 1:17636302/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGaa > 1:6716485/1‑66 (MQ=255) cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCt > 1:19439741/1‑68 (MQ=255) cACCGTGACCGGCCTTTCCGGGCGTCAAACCCCCCGGCGCGCGGCAACCC‑GGGCGCCACGGCGAATc < 1:877565/67‑1 (MQ=255) aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCt > 1:6315852/1‑67 (MQ=255) aCCGTGATCGGCCTTTCCCGGCGCCTCACCACCCGCCGCGCGGCAAACG‑GTGCGCCACGGCGAATCtt < 1:34833501/68‑1 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCtt > 1:16229476/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCtt > 1:1535860/1‑67 (MQ=255) ccGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCtt > 1:24130489/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:281048/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:21615087/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:32333622/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:23734913/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:33288264/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:3746651/1‑67 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTc > 1:18260183/1‑67 (MQ=255) gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTccc > 1:22931988/1‑68 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTccc > 1:2964434/1‑66 (MQ=255) aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACt > 1:30913587/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACtt > 1:10609019/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACtt > 1:14776578/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACtt > 1:6893798/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACtt > 1:8519527/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑CTGCGCCACGGCGAATCTTCCCACtt > 1:29087745/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCCCTTGCg > 1:3190593/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:755561/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:11808831/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCg > 1:14478264/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:12858302/1‑68 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:31563333/1‑68 (MQ=255) ttttCCGGCGTCTCATCACCGGGCGCGGGGTAAACC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTc < 1:33560458/68‑1 (MQ=255) ttttCCGGCGCCTCAACACCCGGCGCGCGGCAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTc < 1:22581878/68‑1 (MQ=39) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:5546945/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:4717861/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:9767721/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:13379191/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:13641501/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:15117696/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:23952999/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:17464863/1‑68 (MQ=255) gggCGCCTCACCACCCGGCGCGGGGCAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:27711646/67‑1 (MQ=38) cGGGGTCTCATCACCCGGCGCGGGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:21179056/68‑1 (MQ=255) cGGCGTCTCATCACCCGGCGCGCGGGAACCC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:30462072/68‑1 (MQ=255) ggCGTCTCCCCCCCCGGCGGGCGGCAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:8246450/68‑1 (MQ=37) ggCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:7407724/1‑67 (MQ=255) gcgcCTCACCCCCCGGCGCGGGGTAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:26189196/67‑1 (MQ=255) gCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:4621118/67‑1 (MQ=255) gCGTCTCAGCACCGGGCGCGCGGTAACCC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:7892248/67‑1 (MQ=255) gCGTCTCACCCCCCGGCG‑GGGGGAAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:90997/67‑1 (MQ=255) cGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:15450352/1‑65 (MQ=255) cGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:19551496/1‑65 (MQ=255) cctcAACCCCGGGCGGGCGGGACACC‑GGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTg < 1:10014445/66‑1 (MQ=255) ctcACCCCCCGGCGCTCGGTAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:33922739/67‑1 (MQ=255) ctcACCCCCCGGCGCGGGGCAACCC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:27869013/67‑1 (MQ=255) ctcAACACCCGGCGCGGGGTAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:27298340/67‑1 (MQ=255) tcatccCCGGGCGCGCGGTAACCG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:22146016/67‑1 (MQ=255) tcatcaCCCGGCGCGCGGGAACCG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAct < 1:24776038/68‑1 (MQ=255) acatccCCCGGCGCGCGGCAACCC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:10819508/66‑1 (MQ=255) atccCCCGGCGGGCGGCAACCC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:30095173/68‑1 (MQ=255) atccCCCGGCGCGCGGGAACCG‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:19142792/68‑1 (MQ=255) atcaCCCGGCGGGGGGGAAACC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:34351722/68‑1 (MQ=255) aacaCCCGGCGCGCGGCAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:23316547/66‑1 (MQ=255) ccccGGCGGGGGGGAACCG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:4215367/66‑1 (MQ=255) aCCGGGCGCGGGGGAAACC‑GGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:28465679/67‑1 (MQ=255) aCCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:18072337/1‑68 (MQ=255) aCCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:620281/1‑68 (MQ=255) aCCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:25208027/1‑68 (MQ=255) aCCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:1276265/1‑68 (MQ=255) cccGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:20401998/1‑68 (MQ=255) ccGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:19939981/1‑68 (MQ=255) ccGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:11246394/1‑68 (MQ=255) ccGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:29520980/1‑68 (MQ=255) ccGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:15828173/1‑68 (MQ=255) ccGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTAGCGCTCACGGGGCTGACTCTGTTTg > 1:25463418/1‑68 (MQ=255) cgcgcgGGAAACC‑GTGCGCCACGGCGATTCTTCCCACTTGCGGTCACGGGGCTGACTCGGTTTGTCa > 1:14098923/1‑67 (MQ=255) aCC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg > 1:28328980/1‑68 (MQ=255) aCC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg < 1:20055800/68‑1 (MQ=255) | AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |