| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,762,278 | G→A | 14.7% | L85L (CTG→CTA) ‡ | yfjQ → | CP4‑57 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,762,278 | 0 | G | A | 14.7% | 1113.5 / 169.8 | 421 | L85L (CTG→CTA) ‡ | yfjQ | CP4‑57 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (226/133); new base A (55/7); total (281/140) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.25e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
Only 100 of 559 total aligned reads displayed.
GCCATTCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACG > CP009273/2762212‑2762345 | gCCATTCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATAtgctgc > 1:330859/1‑68 (MQ=255) cATTCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATAtgctgc < 1:20748126/66‑1 (MQ=255) tatttGCCTGTCAGCCACGGGGAGGTGACCCCCGTCGTCGTGAGGATTCTAAGCATATGCTGCGCCTa < 1:32376936/66‑1 (MQ=25) tCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCt > 1:5663189/1‑67 (MQ=255) cTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTAc < 1:21915774/68‑1 (MQ=255) tttGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACg < 1:15376274/68‑1 (MQ=255) ttGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGt < 1:11497423/68‑1 (MQ=255) ttGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGt < 1:27686083/68‑1 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:25749429/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:10827154/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:598373/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:26831351/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:14430521/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:18223561/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGGCGTGAGCATACTAAGCATATTCTGCGCCTACGTCgt > 1:28236155/1‑67 (MQ=255) cTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg < 1:26645076/67‑1 (MQ=255) tCAGACCCGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAgg > 1:4911190/1‑68 (MQ=255) acAGACACGCGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATAAGCTGCGCCTACCTCGGGAgg > 1:31170905/2‑68 (MQ=255) cAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAggg < 1:3744753/68‑1 (MQ=255) cAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAggg < 1:16760626/68‑1 (MQ=255) cAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAggg < 1:1337079/68‑1 (MQ=255) cacGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGc < 1:712007/66‑1 (MQ=255) cacGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGc < 1:1305292/66‑1 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTAGGTCGGGAGGGGCaaa > 1:27559602/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:16856789/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:22806186/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa < 1:28745745/68‑1 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa < 1:28963075/68‑1 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:34312468/1‑68 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt > 1:17447716/1‑68 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt > 1:2931226/1‑68 (MQ=255) gACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:31309916/1‑68 (MQ=255) aCCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:6522539/1‑67 (MQ=255) cccccGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAAc > 1:16013827/1‑68 (MQ=255) cccccGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAAc > 1:19743859/1‑68 (MQ=255) ccGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAgg < 1:33395152/68‑1 (MQ=255) gtgtGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:15178376/68‑1 (MQ=255) gtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:25997970/68‑1 (MQ=255) gtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:30780246/68‑1 (MQ=255) gCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTa > 1:32438826/1‑68 (MQ=255) aTACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:25467685/1‑67 (MQ=255) tACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:12627807/1‑66 (MQ=255) tACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATcc > 1:21410920/1‑68 (MQ=255) aCTAAGCATATGCTGCGCCTACGTCGGGAGGGGAAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:26302821/1‑65 (MQ=255) cTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCt > 1:18486239/1‑67 (MQ=255) cTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTg < 1:15622412/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:9280066/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:8916047/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:14772896/68‑1 (MQ=255) aaGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCt > 1:31541674/1‑68 (MQ=255) aaGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCt > 1:29572198/1‑68 (MQ=255) aGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCtt > 1:12858648/1‑68 (MQ=255) gCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:10034764/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:17193476/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:32016544/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:14179275/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:6671434/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:270432/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:19468235/1‑68 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATGCTGCTTa > 1:33993666/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:29719747/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:24078713/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:10979699/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:11171839/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:20468466/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:17849792/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:8035364/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:18678621/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:2856538/1‑67 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAAc < 1:4642141/68‑1 (MQ=255) tatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAAct < 1:23101991/68‑1 (MQ=255) tatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAAc > 1:22163100/1‑67 (MQ=255) tatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAAc > 1:24728653/1‑67 (MQ=255) taggctgcGCCTCCGTCGTGAGGGGCAAATTACCGGTAAACCGGTTCCGGAAATTATCCTGCTTAAct < 1:10319220/65‑1 (MQ=255) atGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc < 1:13700081/68‑1 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActct < 1:8373956/68‑1 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:27926263/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:5406577/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:26591910/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:22431676/1‑67 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:11793163/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:18059418/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:7452361/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:34571840/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:26733988/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:12043985/1‑68 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActcgc > 1:20975141/1‑65 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:8759827/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:7815087/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:9800992/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:16428262/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:20022594/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:5291392/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:25142649/1‑67 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCa < 1:23606634/67‑1 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCa < 1:33629490/67‑1 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCAc > 1:1762603/1‑68 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCAc > 1:30048036/1‑68 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCAc > 1:3030181/1‑68 (MQ=255) gcgcCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACg < 1:4153198/68‑1 (MQ=255) | GCCATTCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACG > CP009273/2762212‑2762345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |