| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 104,041 | G→T | 11.7% | intergenic (+80/‑151) | lpxC → / → secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 104,041 | 0 | G | T | 11.7% | 1162.3 / 33.8 | 393 | intergenic (+80/‑151) | lpxC/secM | UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation |
| Reads supporting (aligned to +/- strand): ref base G (94/248); new base T (47/3); total (141/252) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.11e-20 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 417 total aligned reads displayed.
TGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAT > CP009273/103974‑104108 | tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTg > 1:29292152/1‑68 (MQ=255)tGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTg > 1:2570429/1‑68 (MQ=255) gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTCTGa > 1:14900517/1‑68 (MQ=255) gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTgt > 1:31342549/1‑67 (MQ=255) cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAt > 1:7370386/1‑68 (MQ=255) cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAt > 1:24181516/1‑68 (MQ=255) cGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTGatt > 1:20470007/1‑66 (MQ=255) gTATAAAATTCGACTGGCCCATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCCTTTTTTTTTGAAtt < 1:23666492/68‑1 (MQ=255) gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtt < 1:16848883/68‑1 (MQ=255) gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtt < 1:13265180/68‑1 (MQ=255) gTAAAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtt < 1:26767553/68‑1 (MQ=255) ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtttt < 1:29816611/68‑1 (MQ=255) taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTta < 1:17405490/68‑1 (MQ=255) aaaaTTCGACTGGCAAATCTGGCTCTCTCTCCGGACAGGTGAACCAGTCGTTTTTTTTTTAAattttt > 1:20258141/1‑66 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTAATTTtat > 1:34239224/1‑68 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGTAtttttt > 1:11089854/1‑66 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtttttt > 1:34704005/1‑66 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtttttt > 1:5498422/1‑66 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAAtttttt > 1:24826703/1‑66 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTtat > 1:4068671/1‑68 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTtat > 1:12884471/1‑68 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTTAAtttttt > 1:19435689/1‑66 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTTAATTTtat > 1:29066856/1‑68 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTTAATTTtat > 1:23119876/1‑68 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTGAATTTtat > 1:6267068/1‑68 (MQ=255) aaaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTGTTTTTGAAtttttt > 1:18722932/1‑66 (MQ=255) aTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTGTATTTTATAag > 1:17916581/1‑68 (MQ=255) ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAaga < 1:5869193/68‑1 (MQ=255) tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAagag < 1:6582090/68‑1 (MQ=255) tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAagag < 1:13941370/68‑1 (MQ=255) tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAagag < 1:1235016/68‑1 (MQ=255) cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGc < 1:21656057/68‑1 (MQ=255) aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCta > 1:16697779/1‑68 (MQ=255) aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTGAATTTTTTAAGAGcga > 1:25411715/1‑66 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCATTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:26613788/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:10283895/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:8747545/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:32987829/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:30424246/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:27210321/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:30123727/68‑1 (MQ=255) cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCtat < 1:18120946/68‑1 (MQ=255) ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATaa < 1:5354451/68‑1 (MQ=255) cTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGGGCTATAAAAAAAgg > 1:14805542/1‑68 (MQ=255) cTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCGATAAAAAACgg > 1:15456151/1‑68 (MQ=255) cTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTTTATTTTTTAAAAGCTATAAAAAAccg > 1:20066872/1‑66 (MQ=255) tGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGt < 1:31694146/68‑1 (MQ=255) ggCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTg < 1:7640626/68‑1 (MQ=255) aCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAATAGCTATTAAAAACCGTGCGa > 1:17485590/1‑68 (MQ=255) aCTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCgc > 1:19084119/1‑67 (MQ=255) aCTCTCTCCGGCCAGGTGAACCAGTCGTGTTTTTTTGAATTTTTTAAGAGCTATAAAAAACGGTGCGa > 1:8049346/1‑68 (MQ=255) tctctcCGGCCAGGTGAACCAGTCGTTTTTTTTTTAATTTTTTAATAGCTATAAAAAAACGTGCGAAc > 1:18333519/1‑68 (MQ=255) tctcCGGCCAGGTTAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGc < 1:32457916/68‑1 (MQ=255) ctcCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt < 1:32614681/68‑1 (MQ=255) ctcCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt < 1:16506692/68‑1 (MQ=255) ctcCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt < 1:11319132/68‑1 (MQ=255) ctcCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt < 1:33433165/68‑1 (MQ=255) ctcCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCt < 1:33709988/68‑1 (MQ=255) ccGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt < 1:6809390/68‑1 (MQ=255) ccGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt < 1:18415846/68‑1 (MQ=255) ccGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt < 1:9478464/68‑1 (MQ=255) gCCAGGTGAACCAGTCGTTTTTTTTTTAATTTTATAAGAGCTATAAAAAAAGGTGCGTACGGAGtttt > 1:7518900/1‑68 (MQ=255) gCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAATAGCTATAAAAAACGGTGCGCACGCTGttt > 1:33102006/1‑67 (MQ=255) gCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt > 1:15923966/1‑67 (MQ=255) gCCAGGTGAACCAGTCGTGTTTTTTTTAATTTTATTATAGCTATTAAAAAAGGTGCGGACGCGGgttt > 1:49003/1‑68 (MQ=255) ccAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt < 1:3967311/67‑1 (MQ=255) cAGGTGAACCAGTCGTTTTTTTTTTTATTTTAAAAAAAAAATAAAAAAACGTGGCAACGCTGttttat > 1:22047623/1‑66 (MQ=255) cAGGTGAACCAGTCGTTTTTTTTTGAATTTTTTAAGAGCTATAAAAAACCGTGCGAAAGCTGttttgt > 1:14428259/1‑66 (MQ=255) cAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCGGTTTTCt > 1:5833804/1‑68 (MQ=255) tGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCGGTTTTCTTaa > 1:17011303/1‑67 (MQ=255) gAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc < 1:13593610/68‑1 (MQ=255) ccAGTCGTTTTTTTTTTAATTTTATAAGAGCTATAAAAAACGGTGCGAACGGAGTTTTATTAAGCACt > 1:28237262/1‑68 (MQ=255) ccAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt < 1:22633740/68‑1 (MQ=255) ccAGGCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt < 1:28981186/68‑1 (MQ=255) cAGTCGTTTTTTTTTTAATTTTATAAGCGCTATAAAAAACGGTGCGCACGCTGTTTTCTTAAGCACtt > 1:26251076/1‑68 (MQ=255) cGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt < 1:7162018/65‑1 (MQ=255) cGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc < 1:9714857/68‑1 (MQ=255) cGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc < 1:28707914/68‑1 (MQ=255) ttttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGc < 1:4356201/67‑1 (MQ=255) ttttttGAATTTTATAAGTGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:19637268/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:20789611/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:31999710/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:10606287/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:27059918/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:30253090/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca > 1:29473882/1‑68 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:11520148/68‑1 (MQ=255) ttttttGAATTTTATAAGAGCTATAAAAAACGGGGCGAACGCTGTTTTCTTAAGCACTTTTCCGcaca < 1:34386618/68‑1 (MQ=255) tttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACaa < 1:22280103/68‑1 (MQ=255) ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc < 1:28510919/68‑1 (MQ=255) ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc < 1:3079999/68‑1 (MQ=255) ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc < 1:15690017/68‑1 (MQ=255) ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc < 1:8502737/68‑1 (MQ=255) ttttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAAc < 1:10172662/68‑1 (MQ=255) tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt < 1:5660994/68‑1 (MQ=255) tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt > 1:21261034/1‑68 (MQ=255) tttGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACt < 1:3559549/68‑1 (MQ=255) gAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt < 1:13717373/68‑1 (MQ=255) gAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt < 1:31051811/68‑1 (MQ=255) gAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAt < 1:24598448/68‑1 (MQ=255) | TGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTAT > CP009273/103974‑104108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |