| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,762,293 | G→A | 10.3% | E90E (GAG→GAA) | yfjQ → | CP4‑57 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,762,293 | 0 | G | A | 10.3% | 1786.7 / 159.8 | 604 | E90E (GAG→GAA) | yfjQ | CP4‑57 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (342/198); new base A (55/7); total (397/207) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-01 | |||||||||||
Only 100 of 619 total aligned reads displayed.
TCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTCGT > CP009273/2762227‑2762360 | aGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAggg > 1:5641599/1‑67 (MQ=255) cacGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGc < 1:712007/66‑1 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:11855398/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:2761273/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:2932678/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa < 1:28963075/68‑1 (MQ=255) aCGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTAcc < 1:21455720/67‑1 (MQ=255) aCGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTAcc < 1:28203190/67‑1 (MQ=255) aCGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTAcc < 1:15215867/67‑1 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGGAAATTACCGGt > 1:11078389/1‑68 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt < 1:26615006/68‑1 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt < 1:20619257/68‑1 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt > 1:17447716/1‑68 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt > 1:28776015/1‑68 (MQ=255) tGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTa > 1:25265406/1‑68 (MQ=255) gACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:3363042/1‑68 (MQ=255) gACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:3971947/1‑68 (MQ=255) cccccGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaatc > 1:34873354/1‑66 (MQ=255) tcgtcgtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGtt > 1:17210782/1‑67 (MQ=255) gtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:13468686/68‑1 (MQ=255) gtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:34525169/68‑1 (MQ=255) gtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:17726481/68‑1 (MQ=255) gtGAGCATACAAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:11604433/68‑1 (MQ=255) gCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTa > 1:4775688/1‑68 (MQ=255) tACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:2594949/1‑66 (MQ=255) aCTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:30180890/1‑65 (MQ=255) cTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCt > 1:18486239/1‑67 (MQ=255) cTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTg < 1:937904/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCt > 1:1788949/1‑66 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:16098467/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:14772896/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:14346317/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:3185385/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAAATATCCt > 1:33170662/1‑66 (MQ=255) aaacATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGc < 1:20907736/64‑1 (MQ=255) aaGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCt > 1:29572198/1‑68 (MQ=255) aaGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCt > 1:31541674/1‑68 (MQ=255) gCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:10034764/1‑68 (MQ=255) gCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:20130125/1‑68 (MQ=255) gCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:7819397/1‑68 (MQ=255) acATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTa > 1:8947264/2‑68 (MQ=255) acATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTa > 1:27887648/2‑68 (MQ=255) acATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCACGAAGTTCCTGAAATTATCCTGCTTa > 1:5137769/2‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:16771914/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:32528368/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:30905236/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:12977463/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:270432/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:8126553/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:19571084/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:6671434/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:4188290/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGATCCTGAAATTATCCTGCTTaa > 1:15632952/1‑68 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACCGGTTCCGGAAATTATCCTGCTTa > 1:16956021/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:33494169/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:24078713/1‑67 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:18288468/1‑66 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:14172434/1‑66 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:21226390/1‑66 (MQ=255) tatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAAct < 1:34045003/68‑1 (MQ=255) tatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAAc > 1:1538417/1‑67 (MQ=255) atGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc < 1:13700081/68‑1 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGTTTCAGGAAATTATCCTGCTTAActc > 1:20404366/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:1655718/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:53538/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:22767423/1‑67 (MQ=255) tgctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAActc > 1:22431676/1‑67 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:26733988/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:2978188/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:12680870/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:12185400/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:7452361/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:26284667/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:3226367/1‑68 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:8759827/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:9800992/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:31203378/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:10718794/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:22042334/1‑67 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCa < 1:10902952/67‑1 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCa < 1:19845283/67‑1 (MQ=255) gcCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGAt > 1:8420731/1‑68 (MQ=255) ccTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATg > 1:21688594/1‑68 (MQ=255) aCGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCa < 1:10311697/68‑1 (MQ=255) aCGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCa < 1:30599487/68‑1 (MQ=255) cGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCa > 1:2838349/1‑67 (MQ=255) cGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCa > 1:11301362/1‑67 (MQ=255) tCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCa > 1:24646520/1‑68 (MQ=255) tCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCa > 1:31354006/1‑68 (MQ=255) cGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAg < 1:6407376/68‑1 (MQ=255) gggATGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGt < 1:25518389/68‑1 (MQ=255) gggAGGGGCAATTTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGt < 1:18671489/68‑1 (MQ=255) gggAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGt < 1:6003980/68‑1 (MQ=255) gggAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGt < 1:28594722/68‑1 (MQ=255) gggAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTACCCTTCTTAACTCTCACGATGGCACCAGt < 1:24929233/68‑1 (MQ=255) gAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTc > 1:2342197/1‑68 (MQ=255) gAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTc > 1:23115721/1‑68 (MQ=255) gAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTc > 1:26394414/1‑68 (MQ=255) gAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTc > 1:1911712/1‑68 (MQ=255) aGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTCg > 1:26847937/1‑68 (MQ=255) | TCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACGATGGCACCAGTTCGT > CP009273/2762227‑2762360 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |