| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,227,068 | A→C | 7.5% | intergenic (+26/+19) | fadH → / ← higA | 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,227,068 | 0 | A | C | 7.5% | 646.4 / 2.5 | 211 | intergenic (+26/+19) | fadH/higA | 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked/antitoxinof the HigB‑HigA toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base A (124/66); new base C (2/17); total (127/84) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.98e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 224 total aligned reads displayed.
CGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTT > CP009273/3227001‑3227135 | cgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCaa < 1:29521844/68‑1 (MQ=255) ggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAAc > 1:6182873/1‑67 (MQ=255) ggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAAc > 1:16209215/1‑67 (MQ=255) ggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAAc > 1:3287784/1‑67 (MQ=255) cAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa > 1:9793533/1‑68 (MQ=255) cAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa > 1:27543589/1‑68 (MQ=255) cAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa > 1:4487344/1‑68 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGACGCGCCGGGGGCAACGCAt < 1:5756019/68‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa < 1:32288363/67‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa < 1:11813147/67‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa < 1:4664635/67‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa < 1:23712511/67‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa < 1:15191351/67‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCa < 1:767947/67‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt < 1:3921078/68‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt < 1:15867502/68‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt < 1:9657772/68‑1 (MQ=255) aaTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt < 1:30825346/68‑1 (MQ=255) aTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt > 1:32711587/1‑67 (MQ=255) aTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt > 1:8962962/1‑67 (MQ=255) aTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt > 1:21959486/1‑67 (MQ=255) aTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt > 1:20572302/1‑67 (MQ=255) aTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCAt > 1:11899740/1‑67 (MQ=255) tGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCg > 1:30182576/1‑68 (MQ=255) tGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCg > 1:24896901/1‑68 (MQ=255) tGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCg > 1:19163082/1‑68 (MQ=255) tGCCCAGGGAACAAGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGAATCCg > 1:26169544/1‑68 (MQ=255) gCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCg > 1:22055759/1‑67 (MQ=255) cccAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGc > 1:11021009/1‑68 (MQ=255) ccAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGc > 1:23175546/1‑67 (MQ=255) ccAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGc > 1:21060721/1‑67 (MQ=255) aGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACg > 1:696390/1‑68 (MQ=255) ggAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:1852063/1‑68 (MQ=255) ggAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:18686017/1‑68 (MQ=255) ggAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:21492375/1‑68 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGTCGGATGCAACGCATCCGGCACGTc > 1:23779142/1‑67 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCACCGCATCCGGCACGTc > 1:5547670/1‑67 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:10382940/1‑67 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:7896930/1‑67 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:17211233/1‑67 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:14773270/1‑67 (MQ=255) gAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTc > 1:24478974/1‑67 (MQ=255) aaCCCGGCGGGCGCTGGATATTTAAATCT‑TTATTTCACGCGCGGTATGCAACGCATCGGGCACGTcgc < 1:19678218/68‑1 (MQ=255) aaCACGGGTGGCGCTGGATATTTAAATGG‑TTATTTGAAGCGCCTGATGAAACGCATCCGGCACGTcgc < 1:34852383/68‑1 (MQ=255) acacGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTcgcg > 1:14950556/1‑68 (MQ=255) cacGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGt > 1:22407365/1‑68 (MQ=255) cGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCGTTGCATCCGGCACGTCGCGTTa > 1:13330163/1‑68 (MQ=38) cGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTa > 1:26379777/1‑68 (MQ=255) cGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTa > 1:3616582/1‑68 (MQ=255) gggggCGCTGTATTTTAAAATGT‑TTTTTTCACGCGCCGTATCCACCCCAGCGGGCACGTCGCGTTaa < 1:7211226/64‑1 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:272926/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:13563082/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:28390557/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:18200077/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:33567598/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:15544696/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:5211240/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTaa > 1:19668073/1‑67 (MQ=255) gCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATACGGCACGTCGCGTTaa > 1:10013573/1‑67 (MQ=255) tGGCGGTGTATATTAAAATCT‑TTTTTTGACGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATc < 1:13701438/67‑1 (MQ=25) tGGCGCTGTATTTTAAAATCG‑TTATTTGACCCGCCGGATGCAACGAAGCGGGCACGTCGCGTTAATc < 1:3163432/67‑1 (MQ=255) tGGCGCGGGATATTAAAAGCG‑TTATTTGAAGCGCGGGAGGCACCGCACCCGGCACGTCGCGTTAATc < 1:34576387/67‑1 (MQ=255) gggCGCTGGATATTAAAATCG‑TTATTTCACGCGCGGGAGCCAACGCACCCGGCACGTCGCGTTAATCa < 1:2017348/67‑1 (MQ=255) cgcTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATa > 1:16493875/1‑68 (MQ=255) gcTGTATATTTAAATGT‑TTTTAAGAACCGGCGCTTGAAAGGCAGCCGGCACGTCGCGTTAATCAATaa < 1:25351349/68‑1 (MQ=255) gcTGTATATTTAAATGG‑TTATTTGAAGCGCCGTATGCAACGCAGCGGGCACGTCGCGTTAATCAATaa < 1:4847045/68‑1 (MQ=255) gcTGGATATTTAAATCG‑TTATTTGAAGCGCGGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaa < 1:33287794/68‑1 (MQ=255) gcTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaa > 1:9811006/1‑68 (MQ=255) gcTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATaa > 1:28737155/1‑68 (MQ=255) gcTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATACGGCCCGTCGCGTTAATCAATaa > 1:32440625/1‑68 (MQ=255) tGGATATTTAAATCT‑TTATTTGAAGCGCCGGATGCAACGCATCGGGCACGTCGCGTTAATCAATaaa < 1:16647124/67‑1 (MQ=255) ttaTTTAAATGG‑TTATTTTACGCCCGGTATGAACGGCTGCGGGCACGTCGCGTTAATCAATAAACAAg < 1:25644649/66‑1 (MQ=255) ataTTTAAATGT‑TTTTTTCACGCGCGGTATCCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAg < 1:22770271/66‑1 (MQ=255) ataTTTAAATCT‑TTTTTTGAAGCGCGGTATGAAACGAATCGGGCACGTCGCGTTAATCAATAAACAAg < 1:27125652/66‑1 (MQ=255) agaTTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg > 1:16918958/1‑68 (MQ=255) taTTTAAATCT‑TTATTTGACGCGCGGTTTGCAACGCATCGGGCACGTCGCGTTAATCAATAAACAAgg < 1:33507518/67‑1 (MQ=255) ttttAAATTGTTTTTTTCACGCGCGGTATGCAACGAATCCGGCACGTCGCGTTAATCAATAAACAAgg < 1:24370490/67‑1 (MQ=255) tttAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCg > 1:12253847/1‑68 (MQ=255) aaaTCT‑TTTTTTTACGCGCGGTTTGCACCGCAGCGGGCACGTCGCGTTAATCAATAAACAAGGCGGga < 1:14827307/68‑1 (MQ=255) aaaTCT‑TTATTTGAAGCGCGGGTTGCAACGAATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGga < 1:11527411/68‑1 (MQ=255) aaaTCT‑TTATTTCACGCGCCGGATCCAACCCATCGGGCACGTCGCGTTAATCAATAAACAAGGCGGga < 1:26865771/68‑1 (MQ=255) aTCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGgaga > 1:24033761/1‑68 (MQ=255) aTCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGgaga > 1:18008547/1‑68 (MQ=255) cG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGaaa > 1:14413436/1‑68 (MQ=255) cG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGaaa > 1:10268817/1‑68 (MQ=255) cG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGaaa > 1:29966894/1‑68 (MQ=255) g‑TTATTTGAAGCGCGGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAAt < 1:31596418/68‑1 (MQ=255) gAAGCGCCGGATGCAACGCATCAGGCCCGGCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCgca > 1:34066975/1‑65 (MQ=255) aGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGGGGGAGAAATGCCGAATcg > 1:28210357/1‑68 (MQ=255) gcCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGt > 1:3512713/1‑68 (MQ=255) gcCGGATGCAACGCATCCGGCACGTCGCGGTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGt > 1:25766118/1‑68 (MQ=255) taTGCAACCCAGCGGGCACGCCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc < 1:23337334/66‑1 (MQ=255) aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa > 1:24704766/1‑68 (MQ=255) aTGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCa > 1:25612324/1‑68 (MQ=255) gaaaCGCTTCGGGCACGTCGCGTTAATCAATAAACAAGGCTGGAGAAATGCCGAATCGCGTTGCCAAt < 1:17393743/66‑1 (MQ=255) gCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt > 1:17041732/1‑68 (MQ=255) gCAACCCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt < 1:20973608/68‑1 (MQ=255) aaCGAATCGGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAttt < 1:5835486/68‑1 (MQ=255) aCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttt > 1:11980150/1‑68 (MQ=255) aCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttt > 1:28898188/1‑68 (MQ=255) | CGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCG‑TTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTT > CP009273/3227001‑3227135 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |