| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,106,955 | C→T | 100% | A66T (GCG→ACG) | glpK ← | glycerol kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,106,955 | 0 | C | T | 96.1% | 2114.6 / 41.9 | 583 | A66T (GCG→ACG) | glpK | glycerol kinase |
| Reads supporting (aligned to +/- strand): ref base C (9/14); new base T (293/265); total (302/281) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.43e-01 | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 585 total aligned reads displayed.
AGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAA > CP009273/4106890‑4107019 | gTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCa > 1:21137841/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGc > 1:17760297/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGc > 1:10541410/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGc > 1:20111991/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGc > 1:22329088/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGc > 1:25182640/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGc > 1:27178209/1‑67 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCa < 1:5921025/68‑1 (MQ=255) ggTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCa < 1:25815430/68‑1 (MQ=255) ggTATCACGCTCGTTCGTAAAACCGATAGCTGAAATATCATCGGAAATGATAACCGCTTTCCTCAGc > 1:27710209/1‑67 (MQ=255) gTTTCACGCTGGTTCGTAATAGCGATAGGTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:4351631/68‑1 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:2545044/67‑1 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:18459087/67‑1 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:2444250/67‑1 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:28542508/67‑1 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:15197408/67‑1 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACt > 1:27378296/1‑68 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACt > 1:28879525/1‑68 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACt > 1:20799935/1‑68 (MQ=255) tttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACt > 1:15337331/1‑68 (MQ=255) ttCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACtt < 1:11274/68‑1 (MQ=255) cACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCt < 1:23427030/68‑1 (MQ=255) aCGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCt > 1:32153907/1‑67 (MQ=255) cGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTa > 1:15783285/1‑67 (MQ=255) gCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTAcc > 1:5745416/1‑68 (MQ=255) cTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCa > 1:9644760/1‑68 (MQ=255) tGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAg < 1:3164822/68‑1 (MQ=255) ggTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGc < 1:3543207/68‑1 (MQ=255) cGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCAc < 1:17545410/54‑1 (MQ=255) aaTACCTATAGCTGCAATTTGATCGGAACTGATATCGTCTTTCGTCAGCACTTCTACCAGCGGGGAGc < 1:33272887/68‑1 (MQ=255) aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGa > 1:6317868/1‑66 (MQ=255) aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGa > 1:24850589/1‑66 (MQ=255) aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAg < 1:14479728/67‑1 (MQ=255) aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAg < 1:24255660/67‑1 (MQ=255) aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGc < 1:33461754/68‑1 (MQ=255) aaTACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGc < 1:30394106/68‑1 (MQ=255) tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCtt > 1:5305266/1‑68 (MQ=255) tACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCtt > 1:3395787/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:34251917/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:17167283/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:8760466/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:30670304/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:29321511/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:12575245/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:26837135/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:20428999/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCttt > 1:14942757/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTCGAGCttt > 1:29984542/1‑68 (MQ=255) aCCGATAGCTGCAATTTGATAGGAACTGATATCGGCTTTCGTCAGCACTTCTAAAAGCGTGGAGCttt > 1:12270615/1‑68 (MQ=255) cGATAGCTGCAATTTGATCGGAACTGATATCGGCATTCGTCAGCCCTTCAACCAGCGTGGAGCTTTgg > 1:8984482/1‑68 (MQ=255) tAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTg > 1:16138766/1‑68 (MQ=255) gCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTgg > 1:26693457/1‑67 (MQ=255) gCTGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCAACCAGCGTGGAGCTTTGGGTgg > 1:5499575/1‑67 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc < 1:15596997/66‑1 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc < 1:1196687/66‑1 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc < 1:19593793/66‑1 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc < 1:6537931/66‑1 (MQ=255) tGCAATTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGc < 1:17956334/66‑1 (MQ=255) aaTTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa < 1:27638242/68‑1 (MQ=255) aaTTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa < 1:10162977/68‑1 (MQ=255) aaTTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa < 1:8294561/68‑1 (MQ=255) aaTTTGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGa < 1:14663334/68‑1 (MQ=255) tttGATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGAtt > 1:10741253/1‑68 (MQ=255) gATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc < 1:12774004/68‑1 (MQ=255) gATCGGAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTcc < 1:2313856/68‑1 (MQ=255) gATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGACCCAGATTTcc < 1:32840226/68‑1 (MQ=255) ggAACTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTg < 1:28740568/68‑1 (MQ=255) aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg > 1:21614510/1‑67 (MQ=255) aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTggg > 1:9168455/1‑67 (MQ=255) aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt < 1:13926474/68‑1 (MQ=255) aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt < 1:2371503/68‑1 (MQ=255) aCTGATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt < 1:34699215/68‑1 (MQ=255) aCTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGt < 1:4007748/68‑1 (MQ=255) gATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt < 1:4496537/68‑1 (MQ=255) gATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt < 1:13154940/68‑1 (MQ=255) gATATCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgt < 1:1652916/68‑1 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt < 1:7745975/68‑1 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:16865491/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:6767267/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:7600486/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:4956812/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt < 1:14231024/68‑1 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:22779649/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:10024932/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:18712481/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:11041029/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:19031595/1‑68 (MQ=255) tatCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:34396011/1‑68 (MQ=255) tCGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCgtgt > 1:31042991/1‑66 (MQ=255) cGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt < 1:7446976/68‑1 (MQ=255) cGGCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt > 1:19280961/1‑68 (MQ=255) ggCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCt > 1:22606100/1‑67 (MQ=255) ggCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa < 1:22040131/68‑1 (MQ=255) gCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa > 1:13419934/1‑67 (MQ=255) gCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa > 1:28185267/1‑67 (MQ=255) gCTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTa > 1:31332983/1‑67 (MQ=255) cTTTCGTCAGCACTTCTACCTGCGGGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAc < 1:30214010/67‑1 (MQ=255) cTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAc < 1:26344677/67‑1 (MQ=255) cTTTCGTCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTAc < 1:7096974/67‑1 (MQ=255) ccAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCaa > 1:11744286/1‑65 (MQ=255) | AGTGGTTTCACGCTGGTTCGTAATACCGATAGCTGCAATTTGATCGGAACTGATATCGGCTTTCGCCAGCACTTCTACCAGCGTGGAGCTTTGGGTGGCCCAGATTTCCATTGGGTCGTGTTCTACCCAA > CP009273/4106890‑4107019 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |