BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,035	A→T	5.0%	noncoding (38/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,035	0	A	T	5.0%	1483.1 / 17.7	468	noncoding (38/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base A (300/138); new base T (5/25); total (305/163)
Fisher's exact test for biased strand distribution p-value = 2.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 481 total aligned reads displayed.

GCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATAT  >  CP009273/691968‑692102

   cTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATc                                                                  >  1:28566021/1‑68 (MQ=255)

       cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                              >  1:10519062/1‑68 (MQ=255)

       cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                              >  1:12782245/1‑68 (MQ=255)

        aaGGTAAGCGTCTTGATTAAATTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCACaa                                                              >  1:32649700/1‑67 (MQ=18)

        aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                              >  1:25857584/1‑67 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGCACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:28130113/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:19924104/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:30839034/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:28045397/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:23497878/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:22514661/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:21962652/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:19510224/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:3633045/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:5217479/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:10311860/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:11221522/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:6271611/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:295134/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:7792645/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                           >  1:12552818/1‑68 (MQ=255)

          ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAATcc                                                           >  1:168503/1‑68 (MQ=255)

            tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                         >  1:15433655/1‑68 (MQ=255)

            tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCgg                                                         >  1:21676053/1‑68 (MQ=255)

             accgagcttttttaaagTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                        <  1:332771‑M2/51‑1 (MQ=255)

              aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGt                                                        >  1:428881/1‑67 (MQ=255)

                gtgcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                     <  1:6786042‑M2/54‑1 (MQ=255)

                gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                     <  1:16343332‑M2/54‑1 (MQ=255)

                gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                     <  1:33767089‑M2/54‑1 (MQ=255)

                gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                      <  1:13375308‑M2/53‑1 (MQ=255)

                gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                      <  1:21000666‑M2/53‑1 (MQ=255)

                ctgcTTGATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                     <  1:27737802/65‑1 (MQ=255)

                cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                       >  1:34059878/1‑66 (MQ=255)

                 tgcttttataattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                     <  1:2496341‑M2/54‑1 (MQ=255)

                 ggctttattaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                    <  1:16027831‑M2/55‑1 (MQ=255)

                 gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                    >  1:31681464/1‑68 (MQ=255)

                 gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                    >  1:23879552/1‑68 (MQ=255)

                 gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                    >  1:32136899/1‑68 (MQ=255)

                 gTCATGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCAACCGGTGCCt                                                    >  1:22869953/1‑68 (MQ=17)

                  tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCtt                                                   >  1:30470165/1‑68 (MQ=255)

                  gcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCt                                                    <  1:887533‑M2/55‑1 (MQ=255)

                    ttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                  >  1:20189668‑M2/11‑67 (MQ=255)

                     tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                <  1:13083545‑M2/59‑1 (MQ=255)

                      ttttaatgTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCg                                               <  1:4788016‑M2/60‑1 (MQ=255)

                      ttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCg                                               <  1:24435456‑M2/60‑1 (MQ=255)

                      gAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                >  1:24676220/1‑67 (MQ=255)

                       tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                              <  1:7302948‑M2/61‑1 (MQ=255)

                       tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                              <  1:3530960‑M2/61‑1 (MQ=255)

                        ttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                             <  1:1806023‑M2/62‑1 (MQ=255)

                        ttaatgTTGCTGGGGTACGAGGATTCGAACCTCGGATTTCCGGAATCAGAATCCGGTGCCTTACCGCt                                             <  1:5919295‑M2/62‑1 (MQ=255)

                        ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTTCCTTACCGCt                                             <  1:25630608‑M2/62‑1 (MQ=255)

                        ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                             <  1:13961768‑M2/62‑1 (MQ=255)

                        aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                             >  1:20706112/1‑68 (MQ=40)

                        aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                             >  1:22283531/1‑68 (MQ=40)

                          aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                           <  1:30676895‑M2/64‑1 (MQ=255)

                          aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                           <  1:25461252‑M2/64‑1 (MQ=255)

                          aaaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                           <  1:29115869/68‑1 (MQ=11)

                           atgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTgg                                          <  1:8667127‑M2/65‑1 (MQ=255)

                            tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         <  1:31955275/67‑1 (MQ=14)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:31803897‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:9585151‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:28724556‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:14579141‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:12094730‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:11663252‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:20482253‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:26575364‑M2/3‑68 (MQ=255)

                            tgTGGCTGGGGTACGAGGATTAGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         >  1:5565915‑M2/3‑68 (MQ=255)

                            tgTGGCGGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                         <  1:9257688‑M2/66‑1 (MQ=255)

                            aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGc                                         <  1:6527626/68‑1 (MQ=255)

                            aTTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAATCCGGTGCCTTACCGCTTGGc                                         <  1:10927257/68‑1 (MQ=11)

                             ttGGCTGGGGTACGAGGATTCGAACCTCTGAATTAATGAAAGAGAATCCCGAGCCTTACAGCTTGGcc                                        >  1:17457667/1‑67 (MQ=2)

                             ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCg                                        >  1:26262028/1‑68 (MQ=11)

                             ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGCg                                        >  1:2263482/1‑68 (MQ=255)

                             ttGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGTATCATAATCCGGTGCCTTACCGCTTGGCg                                        <  1:6971699/68‑1 (MQ=11)

                                          gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagt                           >  1:18888291/1‑66 (MQ=14)

                                          gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaa                           >  1:27070493/1‑68 (MQ=35)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggt                          >  1:10000107/1‑65 (MQ=14)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaatt                          >  1:30059286/1‑66 (MQ=18)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:8092053/1‑68 (MQ=37)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:26680373/1‑68 (MQ=37)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:27300144/1‑68 (MQ=37)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:33047393/1‑68 (MQ=37)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:4520596/1‑68 (MQ=37)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:20059862/1‑68 (MQ=37)

                                           aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                          >  1:31340249/1‑68 (MQ=37)

                                            ggATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAATaaaat                         >  1:15156678/1‑66 (MQ=9)

                                               ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                      <  1:5973896/68‑1 (MQ=255)

                                                        cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt             <  1:2123454/68‑1 (MQ=255)

                                                            aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg         >  1:2569408/1‑68 (MQ=255)

                                                             tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa        <  1:26861954/68‑1 (MQ=255)

                                                             tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa        <  1:14263392/68‑1 (MQ=255)

                                                              gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa       <  1:29944217/68‑1 (MQ=255)

                                                              gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa       <  1:17027727/68‑1 (MQ=255)

                                                               ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc      >  1:17679216/1‑68 (MQ=18)

                                                                 ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat    <  1:3961805/68‑1 (MQ=255)

                                                                 ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat    <  1:22152673/68‑1 (MQ=255)

                                                                 ggAATCAGAATCCGGTACCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat    <  1:15659246/68‑1 (MQ=255)

                                                                   aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata   <  1:575822/67‑1 (MQ=255)

                                                                   aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata   <  1:34562851/67‑1 (MQ=255)

GCACTTACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATAT  >  CP009273/691968‑692102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`