| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,226 | T→G | 14.9% | V58V (GTT→GTG) | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,226 | 0 | T | G | 14.9% | 1512.5 / 238.9 | 547 | V58V (GTT→GTG) | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (180/285); new base G (35/47); total (215/332) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.40e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-01 | |||||||||||
Only 100 of 594 total aligned reads displayed.
CTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATC > CP009273/4264159‑4264292 | cTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGtt > 1:2658648/1‑68 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:2024318/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:6545860/68‑1 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:1855688/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:9111260/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:16380014/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:8017208/1‑65 (MQ=255) ccGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:31227130/1‑64 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:31876352/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:10002105/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:33041855/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:28475094/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:21272150/68‑1 (MQ=255) cGAATCCTGGAGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:7761609/68‑1 (MQ=255) tCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:27315225/1‑67 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:8291167/2‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:28900968/2‑68 (MQ=255) ccTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGc > 1:2771612/1‑68 (MQ=255) cTGGCGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCa > 1:15361875/1‑68 (MQ=255) cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCaa > 1:1372324/1‑65 (MQ=255) cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCaa > 1:27002315/1‑65 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACATAATGGCATCGCGTGGTGCTGTTCGGCaagc < 1:16703126/67‑3 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc > 1:30361170/1‑65 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:3146714/68‑1 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:13044213/68‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:5965423/67‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:1514337/67‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:8565948/67‑1 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg > 1:33875702/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:10656899/1‑68 (MQ=255) cGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcga < 1:20672433/68‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:33434868/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:22513487/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:32583949/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:29388604/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:14316076/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:15565318/67‑1 (MQ=255) gagaTGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCACACTGGCAGAAGTGgcgagcga < 1:21348995/68‑1 (MQ=255) gagaTGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagcga < 1:31032506/68‑1 (MQ=255) tGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAata < 1:4488751/67‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTTGCAGAAGTGGCGAGCGAATATCTGc < 1:9316309/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:491760/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:12154466/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:12446053/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:21743080/68‑1 (MQ=255) aGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGc < 1:7384363/68‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGGTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:13518995/64‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:24471311/64‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:11576281/64‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:14638625/64‑1 (MQ=255) gagcagacagaATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:20477032/64‑1 (MQ=255) gagcagacagaATGGCACCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGc < 1:12694144/64‑1 (MQ=255) aaCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAat > 1:15027333/1‑60 (MQ=255) aaCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGt > 1:20889398/1‑68 (MQ=255) aCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCg < 1:7050252/66‑1 (MQ=255) aCAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTa > 1:1719800/1‑68 (MQ=255) cAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTaa > 1:9739691/1‑68 (MQ=255) gACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAg > 1:17540030/1‑68 (MQ=255) cagAATGGCATCTCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCaag < 1:23461949/63‑2 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:8820418/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:5604755/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:29874128/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:24619431/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:18257081/1‑68 (MQ=255) gAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTtc < 1:23849260/68‑1 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa < 1:29527020/68‑1 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:11894558/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:18814388/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:24169458/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg > 1:25319530/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg > 1:2915751/1‑68 (MQ=255) gCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg > 1:3615621/1‑68 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:18658634/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:26093130/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:19087025/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:6160109/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:34472042/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:19914402/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:17870815/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:17021203/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:16313668/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:15779541/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:25742795/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTATCAgg < 1:5413525/67‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:27981904/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:16163512/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:24856371/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:11328819/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:34242453/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:21588972/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:22681261/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:30480504/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:32179593/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGt < 1:10902783/67‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGt < 1:4238655/67‑1 (MQ=255) tcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGtct < 1:10443284/67‑3 (MQ=255) cgcgTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTta > 1:14060915/1‑68 (MQ=255) gcgTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTtat < 1:9487364/68‑1 (MQ=255) gcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCtac < 1:33565881/68‑2 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATAACTGCGTAAAGGTTCTCAGGTTtata < 1:19306012/67‑1 (MQ=255) | CTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATC > CP009273/4264159‑4264292 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |