BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,040	G→A	7.1%	noncoding (33/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,040	0	G	A	7.1%	1374.4 / 48.1	479	noncoding (33/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base G (275/150); new base A (9/24); total (294/185)
Fisher's exact test for biased strand distribution p-value = 4.68e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 485 total aligned reads displayed.

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTC  >  CP009273/691973‑692104

tACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAg                                                                  >  1:20180439/1‑68 (MQ=255)

  cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:9722551/1‑68 (MQ=255)

  cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:12782245/1‑68 (MQ=255)

  cAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:10519062/1‑68 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:31837654/1‑67 (MQ=255)

   aaGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGaa                                                                >  1:31459326/1‑67 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGCACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:28130113/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCaaaaac                                                              >  1:22137658/1‑65 (MQ=21)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:10311860/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:8227072/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:22514661/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:22684509/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:23018814/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:23497878/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:24519453/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:27304158/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:28045397/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:32535241/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:33262847/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:34095740/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:5217479/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:5759221/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:599148/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:11221522/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                             >  1:15856422/1‑68 (MQ=255)

     ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCCGAATGCCGGAATCAGAATcc                                                             >  1:2658737/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:194898/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:3562652/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:30797266/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                           >  1:12257846/1‑68 (MQ=255)

       tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCgg                                                           >  1:21676053/1‑68 (MQ=255)

           gatcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGcc                                                       <  1:2544780‑M2/54‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:33767089‑M2/54‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGc                                                        <  1:24893403‑M2/53‑1 (MQ=255)

           gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGc                                                        <  1:26990489‑M2/53‑1 (MQ=255)

           cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                         >  1:5602404/1‑66 (MQ=255)

            ggcTTGATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                       <  1:9404474/65‑1 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAGAATCCGGTg                                                         >  1:25854238/1‑64 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:22270696/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:9042908/1‑68 (MQ=255)

             tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                     >  1:25550851/1‑68 (MQ=255)

                tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                  <  1:8756605‑M2/59‑1 (MQ=255)

                 ttttaatgTGGCTGGTGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCg                                                 <  1:4788016‑M2/60‑1 (MQ=255)

                 ttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCg                                                 <  1:20995344‑M2/60‑1 (MQ=255)

                   ttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCt                                               <  1:20584322‑M2/62‑1 (MQ=255)

                   ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCt                                               <  1:3767511‑M2/62‑1 (MQ=255)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               <  1:4292637/68‑1 (MQ=40)

                   aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                               >  1:30947217/1‑68 (MQ=40)

                    taatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCtt                                              >  1:13357439‑M2/6‑68 (MQ=255)

                     aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                             >  1:30580134‑M2/5‑68 (MQ=255)

                     aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                             >  1:59210‑M2/5‑68 (MQ=255)

                     aaaTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                             >  1:16115514/1‑68 (MQ=37)

                      atttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTgg                                            <  1:6846606/66‑1 (MQ=11)

                       tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                           <  1:26831455/67‑1 (MQ=11)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:7114010‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:34600596‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:6677837‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:24282728‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:17078443‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:20029189‑M2/66‑1 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:4544145‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:7879111‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:14395054‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           >  1:11663252‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCATGGc                                           >  1:27131627‑M2/3‑68 (MQ=255)

                       tgTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGc                                           <  1:28700015‑M2/66‑1 (MQ=255)

                       aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                           <  1:26506243/68‑1 (MQ=33)

                       aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGc                                           <  1:28871158/68‑1 (MQ=255)

                       aTTGGCTGGGGTACGAGGATTCGAACCACGGAATGCCGGAATCAAAATCCGGTGCCTTACCGCTTGGc                                           <  1:10927257/68‑1 (MQ=11)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:29394734/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:7516050/1‑68 (MQ=31)

                        ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                          >  1:1552021/1‑68 (MQ=31)

                                   aCGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaaca                               <  1:25660293/68‑1 (MQ=31)

                                     gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagt                             >  1:18888291/1‑66 (MQ=14)

                                     gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaa                             >  1:32927950/1‑68 (MQ=35)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggt                            >  1:10000107/1‑65 (MQ=14)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggg                            >  1:30024392/1‑65 (MQ=1)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCaaaaaaa                            >  1:17939112/1‑67 (MQ=9)

                                      aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                            >  1:23724451/1‑68 (MQ=37)

                                        gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                          >  1:6494342/1‑68 (MQ=40)

                                          ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                        <  1:152825/68‑1 (MQ=255)

                                               aCCTCGGAATGCCGGAATCAGAATCCTGTGACTTAAAGCTTGCAGATACCCCAACAAAATTGTGTTGa                   >  1:29890481/1‑68 (MQ=2)

                                               aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                   >  1:16512316/1‑68 (MQ=255)

                                               aCCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGa                   >  1:26149352/1‑68 (MQ=255)

                                                   cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt               <  1:2123454/68‑1 (MQ=255)

                                                   cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt               <  1:16953954/68‑1 (MQ=255)

                                                   cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt               <  1:27760405/68‑1 (MQ=255)

                                                       aTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCg           >  1:13082434/1‑68 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa          <  1:32112799/68‑1 (MQ=255)

                                                        tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa          <  1:12567932/68‑1 (MQ=255)

                                                          ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGGCGatc        >  1:17637282/1‑66 (MQ=17)

                                                           cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc        <  1:2189171/67‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat      <  1:14724723/68‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat      <  1:4006276/68‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat      <  1:31692686/68‑1 (MQ=255)

                                                            ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat      <  1:19909532/68‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata     <  1:14105936/67‑1 (MQ=255)

                                                              aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata     <  1:20473363/67‑1 (MQ=255)

                                                               aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat    <  1:30153335/67‑1 (MQ=255)

                                                                tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTc  >  1:13725091/1‑68 (MQ=255)

TACAAGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTC  >  CP009273/691973‑692104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`