| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,274 | A→G | 10.3% | K74K (AAA→AAG) | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,274 | 0 | A | G | 10.3% | 2265.5 / 179.4 | 712 | K74K (AAA→AAG) | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (205/431); new base G (24/49); total (229/483) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.95e-01 | |||||||||||
Only 100 of 828 total aligned reads displayed.
AGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATC > CP009273/4264207‑4264341 | cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt < 1:24478295/68‑1 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:19718289/1‑68 (MQ=255) cTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGt > 1:19296234/1‑68 (MQ=255) gAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTtc < 1:23849260/68‑1 (MQ=255) gAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTtc < 1:8585503/68‑1 (MQ=255) aTGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAggg < 1:25895092/63‑1 (MQ=255) tGGCACCGTGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:11450043/1‑68 (MQ=255) tGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCa > 1:28570333/1‑68 (MQ=255) cACCGCGTTGTTCTGTTCGGCAAACTTTCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:14101625/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:17021203/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:7401722/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:8455844/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:542871/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:27851307/67‑1 (MQ=255) cACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAgg < 1:34204148/67‑1 (MQ=255) atcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGt < 1:24276615/65‑1 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:3338580/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:31260688/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:16163512/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGtt > 1:10881199/1‑68 (MQ=255) aCCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGt < 1:11874929/67‑1 (MQ=255) tcgcgTGGTGCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGtct > 1:2345396/2‑66 (MQ=255) gcgTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTtat < 1:14921673/68‑1 (MQ=255) gTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTtata < 1:21601532/67‑1 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:601136/1‑68 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:3788071/1‑68 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:15215350/1‑68 (MQ=255) gCTGTTCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAg > 1:1279531/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGaa < 1:16676754/67‑1 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGaa < 1:18654898/67‑1 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGaa < 1:9156473/67‑1 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:13571387/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:24644386/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:12573419/1‑68 (MQ=255) gCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAg > 1:10534157/1‑68 (MQ=255) tGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAgg < 1:8876724/67‑1 (MQ=255) tCGGCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGG‑‑CGCAGGTCTACATCGAAGGTCAGCt > 1:20331265/1‑68 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:10156750/1‑67 (MQ=255) tCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCa > 1:13101747/1‑67 (MQ=255) cGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:30414308/68‑1 (MQ=255) cGGAAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGc < 1:34501026/68‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:17303736/68‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:8746213/68‑1 (MQ=255) ggCAAGCTCGCGGAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCt < 1:12342087/68‑1 (MQ=255) ggCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCt < 1:29928393/68‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGc < 1:32458263/67‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGc < 1:8024950/67‑1 (MQ=255) aaaCTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCg > 1:29124981/1‑68 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:3187780/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:11618794/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:30983352/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:31600735/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:21336786/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:25074787/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:13188900/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:649307/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:5801645/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:20114220/68‑1 (MQ=255) tGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAcc < 1:4189101/68‑1 (MQ=255) ggCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAccc < 1:25397556/68‑1 (MQ=255) ggCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTAccc < 1:29588697/68‑1 (MQ=255) gCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGt < 1:29450887/41‑1 (MQ=255) ggAAGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTa < 1:9663687/67‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:4676627/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:25676468/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:16367210/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:7757200/65‑1 (MQ=255) aGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:14545232/65‑1 (MQ=255) aGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagc < 1:27052569/67‑3 (MQ=255) aGTGGCAGGTGAATGTCTGCGCAAGGGCGCGCAGGTCTACATCGAAGGTCAGCTTCGCACCCGTagc < 1:30606212/67‑3 (MQ=255) gTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATg > 1:15733794/1‑68 (MQ=255) cgagcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGAcc > 1:26730169/1‑68 (MQ=255) gcgaATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa > 1:14340862/1‑67 (MQ=255) gcgaATATCTGCGTAAAGGCTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGa < 1:10006388/67‑1 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTa < 1:21114889/53‑1 (MQ=255) atatCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCaa < 1:23109324/67‑1 (MQ=255) atCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATcc > 1:18923561/1‑68 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:17869295/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:15479995/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:32970523/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:32694174/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:25250685/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCgg < 1:27293433/68‑1 (MQ=255) cTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGCACCGATCAATCCgg < 1:6979548/68‑1 (MQ=255) gTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAg > 1:9422297/1‑68 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAgg < 1:4380639/67‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAgg < 1:22269405/67‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAgg < 1:27529090/67‑1 (MQ=255) aaaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa < 1:13808670/68‑1 (MQ=255) aaGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGa > 1:11134593/1‑67 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:1826299/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:6278250/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:17520262/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:5146403/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:28128195/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:2078786/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:21967323/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:33621255/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:22674084/68‑1 (MQ=255) aGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATc < 1:26362865/68‑1 (MQ=255) | AGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGCGAATATCTGCGTAAAGGTTCTCAGGTTTATATCGAAGGTCAGCTGCGTACCCGTAAATGGACCGATCAATCCGGTCAGGATC > CP009273/4264207‑4264341 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |