BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,043	T→A	8.2%	noncoding (30/75 nt)	glnV ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,043	0	T	A	8.2%	1396.4 / 35.5	464	noncoding (30/75 nt)	glnV	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (264/157); new base A (14/29); total (278/186)
Fisher's exact test for biased strand distribution p-value = 2.38e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 484 total aligned reads displayed.

AGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691977‑692110

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:22684509/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:22514661/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:108852/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:3171619/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:33190666/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:8227072/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:18908693/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:18113967/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:17020496/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATcc                                                                   >  1:5697773/1‑68 (MQ=255)

 ggTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCCGAATGCCGGAATCAGAATcc                                                                   >  1:2658737/1‑68 (MQ=255)

   tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:30797266/1‑68 (MQ=255)

   tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:16630950/1‑68 (MQ=255)

   tAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCgg                                                                 >  1:34280536/1‑68 (MQ=255)

     aGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCTGAATCAGAATACGGt                                                                >  1:12611522/1‑67 (MQ=255)

       gagcttttttaatgTGGCTGGGGTACGATGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:2994490‑M2/54‑1 (MQ=255)

       gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:3011530‑M2/54‑1 (MQ=255)

       gagcttttttaatgTGGCTGGGGTACGAGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGc                                                              <  1:26990489‑M2/53‑1 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:11201825/1‑66 (MQ=255)

       cGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTg                                                               >  1:23595121/1‑66 (MQ=255)

        ggcTTGATTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGcc                                                             <  1:9404474/65‑1 (MQ=255)

        gTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            >  1:32136899/1‑68 (MQ=255)

        gTCATGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGCAACCGGTGCCt                                                            >  1:22869953/1‑68 (MQ=17)

         tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:26172043/1‑68 (MQ=255)

         tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:9042908/1‑68 (MQ=255)

         tCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCtt                                                           >  1:5279186/1‑68 (MQ=255)

         gcttttttaatgTGGCTGGGGTACGAGGATGCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCt                                                            <  1:3828749‑M2/55‑1 (MQ=255)

           ttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                          >  1:29668665‑M2/11‑67 (MQ=255)

           ttttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTa                                                          >  1:20189668‑M2/11‑67 (MQ=255)

            tttttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTAcc                                                        <  1:8756605‑M2/59‑1 (MQ=255)

             ttttaatgTGGCTGGGGTACGAGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCg                                                       <  1:20995344‑M2/60‑1 (MQ=255)

              tttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGc                                                      <  1:3530960‑M2/61‑1 (MQ=255)

               ttaattTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCt                                                     <  1:20584322‑M2/62‑1 (MQ=255)

               ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     <  1:28310252‑M2/62‑1 (MQ=255)

               ttaatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:2565771‑M2/7‑68 (MQ=255)

               aTAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCt                                                     >  1:22283531/1‑68 (MQ=40)

                tAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCtt                                                    >  1:2740283/1‑68 (MQ=39)

                 aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCATAAACCGGTGCCTTACCGCTTg                                                   <  1:4563456‑M2/64‑1 (MQ=255)

                 aatgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTg                                                   >  1:30580134‑M2/5‑68 (MQ=255)

                  atttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAAACCGGTGCCTTACCGCTTgg                                                  <  1:6846606/66‑1 (MQ=11)

                  aaTTGGCGGGGGTACGA‑GATTCGAACCTCGGAATGCCGGAATCAAAATCCGGTGCCTTACCGCTTGGc                                                 <  1:6147834/68‑1 (MQ=11)

                   tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:26831455/67‑1 (MQ=11)

                   tttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:31955275/67‑1 (MQ=14)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGTATCAAAATCCGGTGCCTTACCGCTTGGc                                                 <  1:19755464‑M2/66‑1 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:30959929‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:7879111‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:7114010‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:4340083‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:27176194‑M2/66‑1 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:580963‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:593793‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:6677837‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:10219137‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:17353010‑M2/66‑1 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:25492272‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:20029189‑M2/66‑1 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:16292393‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:12094730‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:12538760‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:24282728‑M2/3‑68 (MQ=255)

                   tgTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 >  1:14395054‑M2/3‑68 (MQ=255)

                   aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGc                                                 <  1:26506243/68‑1 (MQ=33)

                   aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCACAATCCGGTGCCTTACCGCTTGGc                                                 >  1:14901623/1‑68 (MQ=18)

                   aTTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGc                                                 <  1:28871158/68‑1 (MQ=255)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:7516050/1‑68 (MQ=31)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:3146148/1‑68 (MQ=31)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:33532395/1‑68 (MQ=31)

                    ttGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCg                                                >  1:29394734/1‑68 (MQ=31)

                                 gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacagt                                   >  1:13896203/1‑66 (MQ=14)

                                 gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaa                                   >  1:104352/1‑68 (MQ=35)

                                 gAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACaaa                                   <  1:4040588/68‑1 (MQ=35)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacatgg                                  >  1:15935826/1‑65 (MQ=14)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCcaacaggt                                  >  1:10000107/1‑65 (MQ=14)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:25816493/1‑68 (MQ=37)

                                  aGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAAt                                  >  1:11652498/1‑68 (MQ=37)

                                    gATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTg                                >  1:6494342/1‑68 (MQ=40)

                                      ttCGAACCTCGGAATGCCGTAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:5043457/68‑1 (MQ=255)

                                      ttCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGt                              <  1:34038313/68‑1 (MQ=255)

                                               cGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAAttt                     <  1:2123454/68‑1 (MQ=255)

                                                  aaTGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGc                   <  1:13272825/67‑1 (MQ=255)

                                                    tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:28053042/68‑1 (MQ=255)

                                                    tGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGa                <  1:25256411/68‑1 (MQ=255)

                                                     gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:32852341/68‑1 (MQ=255)

                                                     gCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGaa               <  1:24501650/68‑1 (MQ=255)

                                                      ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc              >  1:17679216/1‑68 (MQ=18)

                                                       cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc              <  1:15391677/67‑1 (MQ=255)

                                                       cGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAAc              <  1:2189171/67‑1 (MQ=255)

                                                        ggAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:22152673/68‑1 (MQ=255)

                                                        ggAATCAGAATCCGGTACCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACat            <  1:15659246/68‑1 (MQ=255)

                                                          aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:20473363/67‑1 (MQ=255)

                                                          aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:28712462/67‑1 (MQ=255)

                                                          aaTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACata           <  1:14105936/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGGGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:19379864/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:12913988/67‑1 (MQ=255)

                                                           aTCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACatat          <  1:24775509/67‑1 (MQ=255)

                                                            tCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGGTTTGAATTTGCCGAACATATTc        >  1:27789954/1‑68 (MQ=255)

                                                                 aTCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATAc   <  1:21610990/68‑1 (MQ=255)

                                                                  tCCGGTGCCTTACCGCTTGGCGGTACCCCAAGAAATTGGTGTTGAATTTTACGATGATATTATATACa  >  1:23444936/1‑68 (MQ=12)

                                                                  tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:13671522/1‑68 (MQ=255)

                                                                  tCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACa  >  1:9720073/1‑68 (MQ=255)

AGGTAAGCGTCTTGAATAAATTGGCTGGGGTACGAGGATTCGAACCTCGGAATGCCGGAATCAGAATCCGGTGCCTTACCGCTTGGCGATACCCCAACAAATTGGTTTTGAATTTGCCGAACATATTCGATACA  >  CP009273/691977‑692110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`