BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	692,358	T→A	5.3%	noncoding (38/75 nt)	glnU ←	tRNA‑Gln

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	692,358	0	T	A	5.3%	811.0 / 8.0	266	noncoding (38/75 nt)	glnU	tRNA‑Gln
Reads supporting (aligned to +/- strand): ref base T (132/118); new base A (3/13); total (135/131)
Fisher's exact test for biased strand distribution p-value = 9.39e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 270 total aligned reads displayed.

CCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTG  >  CP009273/692291‑692423

ccGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                                                                   >  1:31793233/1‑68 (MQ=255)

ccGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                                                                   >  1:482738/1‑68 (MQ=255)

ccGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGt                                                                   >  1:22584467/1‑68 (MQ=255)

 cGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTa                                                                  >  1:9518609/1‑68 (MQ=255)

 cGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTa                                                                  >  1:9236560/1‑68 (MQ=255)

 cGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTa                                                                  >  1:8048034/1‑68 (MQ=255)

      tctGATCCGCTTTCTAGAATAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaa                                                              <  1:17855582/65‑1 (MQ=14)

      ggTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCaaa                                                             >  1:12132016/1‑68 (MQ=255)

         gtaCCGCGTACTCGAATAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAc                                                          <  1:2690313/66‑1 (MQ=17)

         gAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACAAGGGAATGCCGGTATCaaaaa                                                           >  1:24651606/1‑67 (MQ=255)

          aaCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAAcc                                                         >  1:12528481/1‑68 (MQ=255)

           aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGCACCAGGGAATGCCGGTATCaaaaa                                                           >  1:15153691/1‑65 (MQ=255)

           aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                        >  1:6436208/1‑68 (MQ=255)

           aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                        >  1:20704117/1‑68 (MQ=255)

           aCCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCg                                                        >  1:8542614/1‑68 (MQ=255)

              gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                      >  1:33956326/1‑67 (MQ=255)

              gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                      >  1:30268213/1‑67 (MQ=255)

              gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                      >  1:29004790/1‑67 (MQ=255)

              gCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGt                                                      >  1:18656291/1‑67 (MQ=255)

                ttAGTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg                                                     >  1:17505285/1‑66 (MQ=255)

                ttACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg                                                     >  1:6010919/1‑66 (MQ=255)

                ttACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTg                                                     >  1:9917778/1‑66 (MQ=255)

                      gaagaaGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTAcc                                              >  1:12855925/1‑67 (MQ=40)

                          aagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCtt                                          >  1:899365/1‑67 (MQ=33)

                          aagaTGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTg                                         >  1:19116047/1‑68 (MQ=33)

                                        aCCTGGGTTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAtac                           >  1:25150455/1‑66 (MQ=9)

                                        aCCTGGATTCGAACCTGGGTATGCCGGTATGAAAATCCGGTGCCTTACCGCTTGGCGATACCCCATc                            <  1:5231052/67‑1 (MQ=11)

                                        aCCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATc                            <  1:14145413/67‑1 (MQ=33)

                                          gtGGATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         <  1:26552397/67‑1 (MQ=255)

                                          gaggATTCGAACCTGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         <  1:20565236/66‑1 (MQ=255)

                                          cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCAtaac                          >  1:19525120/1‑64 (MQ=14)

                                          cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         <  1:28303862/68‑1 (MQ=39)

                                          cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         <  1:7178449/68‑1 (MQ=39)

                                          cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         <  1:33218393/68‑1 (MQ=39)

                                          cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         <  1:1010867/68‑1 (MQ=39)

                                          cTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         >  1:11404589/1‑68 (MQ=39)

                                           tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         >  1:28039257/1‑67 (MQ=39)

                                           tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         >  1:13687208/1‑67 (MQ=39)

                                           tGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGt                         >  1:32190443/1‑67 (MQ=39)

                                               ttCGAACCTCGGAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    <  1:14228816/68‑1 (MQ=14)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGgacgac                    >  1:29607059/1‑68 (MQ=255)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    <  1:19286559/68‑1 (MQ=255)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    >  1:8326025/1‑68 (MQ=255)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    >  1:29434428/1‑68 (MQ=255)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    >  1:17395842/1‑68 (MQ=255)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    >  1:30776090/1‑68 (MQ=255)

                                               ttCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    >  1:31408044/1‑68 (MQ=255)

                                                tCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAAc                    >  1:33327362/1‑67 (MQ=255)

                                                 cGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGc                  <  1:4090016/68‑1 (MQ=255)

                                                   aaCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTCCCCCGCtt                >  1:4642844/1‑68 (MQ=14)

                                                   aaCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                >  1:31939454/1‑68 (MQ=255)

                                                   aaCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                >  1:21779773/1‑68 (MQ=255)

                                                    aCCTGGGTATGACTGTATCAAAATACGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                <  1:11528350/67‑1 (MQ=17)

                                                    aCCTGGGAATGCCGGTATCATAATCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCttt               <  1:16483909/68‑1 (MQ=18)

                                                    aCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                <  1:14383864/67‑1 (MQ=255)

                                                    aCCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                >  1:27846441/1‑67 (MQ=255)

                                                     ccAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGCTACCCCATCCGTACAACGCtt                >  1:7672604/1‑66 (MQ=255)

                                                     ccAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCtt                >  1:20271665/1‑66 (MQ=255)

                                                      ctgggAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:17142617/66‑1 (MQ=255)

                                                      ctgggAATGCCGGAATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:5739341/66‑1 (MQ=18)

                                                      ctcggAATGCCGGTATCACAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:18315540/65‑1 (MQ=255)

                                                      cacggAATGCCGGTATCAAAAACCGGTGCCTTACCTCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:13312082/65‑1 (MQ=255)

                                                      cacggAATGCCGGAATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:34086500/65‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCTGTGAATTACCGCTTGGCGATACCACATCCGTACAACGCTTTCt             <  1:24495813/68‑1 (MQ=18)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:18791182/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:7419645/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:34381988/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:21525155/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:22212807/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:22681902/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:23927327/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:29897408/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:25247277/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCt             <  1:6125947/68‑1 (MQ=255)

                                                      cAGGGAATGCCGGTACCAAAAACCGGTGCCTTACCGCTTGGCGATCCCCCATCCGTACAACGCTTTCt             <  1:23451794/68‑1 (MQ=18)

                                                         ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt          >  1:33096041/1‑68 (MQ=255)

                                                         ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt          >  1:1694200/1‑68 (MQ=255)

                                                         ggAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGt          >  1:256499/1‑68 (MQ=255)

                                                          gAATGCCGGTATCATAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTgg           <  1:16417898/66‑1 (MQ=255)

                                                          gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTgg           <  1:499264/66‑1 (MQ=255)

                                                          gAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACACCATCCGTACAACGCTTTCTGGTg         >  1:30579244/1‑68 (MQ=255)

                                                            aTGCCGGTATCATAATCCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGa        <  1:20931825/67‑1 (MQ=255)

                                                            aTGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGaa       >  1:3113440/1‑68 (MQ=255)

                                                             tGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAAt      <  1:10445135/68‑1 (MQ=255)

                                                              gCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCCTCCGTACACCGCTTTCTGGTGAATg     >  1:11105919/1‑68 (MQ=255)

                                                              gCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATg     <  1:34717355/68‑1 (MQ=255)

                                                              gCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATg     <  1:31593159/68‑1 (MQ=255)

                                                              gCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATg     >  1:16521446/1‑68 (MQ=255)

                                                               ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATgg    >  1:1593261/1‑68 (MQ=255)

                                                               ccGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATgg    >  1:21358728/1‑68 (MQ=255)

                                                                cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAGCGCTTTCTGGTGAATGGt   <  1:15017044/68‑1 (MQ=255)

                                                                cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATgg    <  1:15415113/67‑1 (MQ=255)

                                                                cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATgg    <  1:11936153/67‑1 (MQ=255)

                                                                cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt   <  1:34528913/68‑1 (MQ=255)

                                                                cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt   <  1:26210470/68‑1 (MQ=255)

                                                                cGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt   <  1:18602168/68‑1 (MQ=255)

                                                                 ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGt   >  1:32402060/1‑67 (MQ=255)

                                                                 ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg  <  1:12795673/68‑1 (MQ=255)

                                                                 ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg  <  1:24317565/68‑1 (MQ=255)

                                                                 ggTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTg  <  1:17919367/68‑1 (MQ=255)

CCGGGCGGTGAACCGCTTACTCGAAGAAGATGGCTGGGGTACCTGGATTCGAACCAGGGAATGCCGGTATCAAAAACCGGTGCCTTACCGCTTGGCGATACCCCATCCGTACAACGCTTTCTGGTGAATGGTG  >  CP009273/692291‑692423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`