| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 66,706 | T→C | 68.8% | intergenic (‑174/‑168) | araD ← / → araC | pseudogene, truncated/ara regulon transcriptional activator; autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 66,706 | 0 | T | C | 68.8% | 2000.4 / inf | 1460 | intergenic (‑174/‑168) | araD/araC | pseudogene, truncated/ara regulon transcriptional activator; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (260/194); new base C (542/462); total (803/657) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.54e-01 | |||||||||||
Only 100 of 1468 total aligned reads displayed.
ATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGG > CP009273/66639‑66773 | aTAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGt > 1:6057342/1‑68 (MQ=255)aTAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGt > 1:29470361/1‑68 (MQ=255)aTAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGt > 1:33555314/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAGTGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:26112246/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAcgc > 1:13835056/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAcgc > 1:8542281/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:30939496/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:14869240/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:22879756/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:27761835/1‑68 (MQ=255) aaaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTAcgc > 1:28539121/1‑68 (MQ=255) aaGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTAcgcg < 1:23258751/68‑1 (MQ=255) gtgtGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGtt > 1:2250492/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGttt > 1:13088660/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGttt > 1:2463648/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGttt > 1:34881846/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGttt > 1:4049259/1‑68 (MQ=255) tgtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGttt > 1:19045132/1‑68 (MQ=255) gtgACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGtttt > 1:7173934/1‑68 (MQ=255) gtgACGCCGTGCAAATAATCAATGTGGACCTTTCTGCCGTGATTATAGACACTTTTGCTACGCGtt < 1:31281353/66‑1 (MQ=255) aCGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTg > 1:3702816/1‑67 (MQ=255) aCGCCGTGCAAATAATCAATGTGGACTTTTAGGCCGTGATTATAGACACTTTTGCTACGCGTTTTTg > 1:5502959/1‑67 (MQ=255) cGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGt < 1:11654492/67‑1 (MQ=255) gCCGTGCGAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTGTGTCa > 1:36199216/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:699365/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:25544127/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:2025934/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:31940144/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCa > 1:6525449/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:11257907/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:28237934/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:32742659/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:29146895/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:9154016/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:31553200/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:8949586/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:8139986/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:35085102/1‑68 (MQ=255) gCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCa > 1:4673157/1‑68 (MQ=255) gCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGcc < 1:15959987/68‑2 (MQ=255) gCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGcc < 1:36481767/68‑2 (MQ=255) gCAAATAATCAATGTGGACTTTTCTGCCGGGATTATATACACTTTTGCTACGCGTTTTTGTCATGGcc < 1:29827728/68‑2 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTgg > 1:14271684/1‑67 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTgg > 1:4417631/1‑67 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGt < 1:27135677/68‑1 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGt < 1:21593484/68‑1 (MQ=255) taatCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTAGCTACGCGTTTTTGTCATGGCCTTgg > 1:22181603/1‑67 (MQ=255) taatCAATGTGGACTTTTCTGCAGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGt < 1:17122747/68‑1 (MQ=255) atCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTc > 1:16417473/1‑67 (MQ=255) tCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTccc > 1:5859695/1‑68 (MQ=255) tCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTccc > 1:18810493/1‑68 (MQ=255) cAATGTGGACTTTTCTGCCGTGTTTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCg < 1:14580296/68‑1 (MQ=255) cAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTccc > 1:34632583/1‑67 (MQ=255) cAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCg < 1:16611765/68‑1 (MQ=255) tgtgGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCtt > 1:9857093/1‑68 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCttt < 1:25875247/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCttt < 1:9526260/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:10983480/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:28795308/68‑1 (MQ=255) gtgGACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCttt < 1:24908839/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGt < 1:192962/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:7511833/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:35567032/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:2677890/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:1344437/68‑1 (MQ=255) ggACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGt < 1:10002049/68‑1 (MQ=255) gACTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGtt > 1:21166449/1‑68 (MQ=255) aCTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGtt > 1:5177667/1‑67 (MQ=255) cTTTTCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTa < 1:178244/67‑1 (MQ=255) tCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGaa > 1:7780726/1‑68 (MQ=255) tCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGaa > 1:21889477/1‑68 (MQ=255) tCTGCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCAAGGCCTTGGTCCCGCTTTGTTACAGaa > 1:19974797/1‑68 (MQ=255) tGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATg < 1:15159059/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGc < 1:36744255/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGc < 1:23920968/68‑1 (MQ=255) gCCGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGc < 1:34322477/68‑1 (MQ=255) cGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCtt > 1:18395943/1‑68 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCt < 1:18162219/67‑1 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCt < 1:20561958/67‑1 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCt < 1:33804592/67‑1 (MQ=255) cGTGATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCt < 1:33152131/67‑1 (MQ=255) gATTATAGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCtttt < 1:12180119/67‑1 (MQ=255) tataGACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTtaata > 1:20908870/1‑68 (MQ=255) gACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCg > 1:2347975/1‑68 (MQ=255) gACACTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCg > 1:27547729/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:32441371/1‑68 (MQ=255) acacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:12896212/1‑68 (MQ=255) acacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:12540470/1‑68 (MQ=255) acacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:383503/1‑68 (MQ=255) acacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgg > 1:25198508/1‑68 (MQ=255) cacTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCggg > 1:17587851/1‑68 (MQ=255) cacTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCg < 1:10751637/66‑1 (MQ=255) acTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgggg > 1:10453701/1‑68 (MQ=255) acTTTTGGTACGCGTTTTTGTCTTGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgggg < 1:30740550/68‑1 (MQ=255) acTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgggg < 1:19635970/68‑1 (MQ=255) acTTTTGCAACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCgggg < 1:24218497/68‑1 (MQ=255) cTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGt < 1:3595596/68‑1 (MQ=255) cTTTTGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGt > 1:26511971/1‑68 (MQ=255) tttGCTACGCGTTTTTGTCATGGCCTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGtt > 1:15570110/1‑67 (MQ=255) tGTTACGAGTTTTTGTCATGGCTTTTGTCCCTCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTAcc > 1:30235979/1‑68 (MQ=255) | ATAGCAAAGTGTGACGCCGTGCAAATAATCAATGTGGACTTTTCTGCCGTGATTATAGACACTTTTGTTACGCGTTTTTGTCATGGCTTTGGTCCCGCTTTGTTACAGAATGCTTTTAATAAGCGGGGTTACCGG > CP009273/66639‑66773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |