| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,711,673 | A→C | 9.6% | V72G (GTT→GGT) | yfiF ← | putative methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,711,673 | 0 | A | C | 9.6% | 849.7 / 14.6 | 318 | V72G (GTT→GGT) | yfiF | putative methyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (213/70); new base C (4/31); total (217/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.68e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 332 total aligned reads displayed.
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 | acaCTTGTCCCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:26781984/66‑1 (MQ=255)aaaCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAAc < 1:34036768/68‑1 (MQ=255) caCTTTTCCAACTGCTGCCACCGTGATCGGCCTTTTCCGGAGTCTCATCACCCGGCGCGCGGGAAAcc < 1:33340880/67‑1 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCCCCCGGCGCGCGGGACACCg > 1:17175828/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:37056049/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:3479988/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:30993207/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:13923142/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:29499083/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:8496489/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:10821712/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:10031158/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:22734383/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:23705/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGATCGGCCTTTTACGGCGTCTCATCACCCGGCGCGCGGGAAACCg > 1:2392035/1‑68 (MQ=255) aCTTTTACCACTGATGCCACCGTGAACGGCCTTTTCCGGCGTCTCATCACCCGGCGCCCGGCAAACCg > 1:520277/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt > 1:35626602/1‑68 (MQ=255) cTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt < 1:31944361/68‑1 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt > 1:36398612/1‑67 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt > 1:15375731/1‑67 (MQ=255) ttttACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt > 1:31613841/1‑67 (MQ=255) ttttACCACTGATGCCACCGTGATAGGCCGTTTCCGGCGTCTCATCACCCGGCGCGCGGGATACCGt > 1:12867965/1‑67 (MQ=255) ttttACCACTGATCCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGt > 1:4101746/1‑67 (MQ=255) ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGcc > 1:10337154/1‑67 (MQ=255) cACTGATGCCCCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:32682206/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCa > 1:10414107/1‑67 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:34373658/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:17719675/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:1662459/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:34840353/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:14751663/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:28940544/1‑68 (MQ=255) cACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCAc > 1:4718195/1‑68 (MQ=255) aCTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCCCCCGGCGCGCGGGAAACCGTGCGCCACg > 1:19248770/1‑68 (MQ=255) tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGc > 1:34468625/1‑68 (MQ=255) tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGc > 1:13255378/1‑68 (MQ=255) cccccGTGAGCGGCCTTTTCCGGCGTCTCAGCACCCGGCGCGCGGCAACCCGGGCGCCACGGCGAATc < 1:23441395/65‑1 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGaa > 1:27180014/1‑66 (MQ=255) ccACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGaa > 1:21461226/1‑66 (MQ=255) ccccGTGATCGGCCTTTGCCGGCGTCTCAACCCCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCt < 1:11246319/66‑1 (MQ=255) cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt > 1:9878911/1‑68 (MQ=255) cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCt > 1:21965181/1‑68 (MQ=255) cACCGTGATCGGCCTTTTCCGGCGTCTCATCACACGGCGCGCGGGAAACCGTGCGCCACGGCGActct > 1:7904769/1‑68 (MQ=255) cGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTc > 1:33588300/1‑67 (MQ=255) gttCGCCCTTTCCCGGCGTCACAACCCCGGGCGCGCGGTAACCGGTGCGCCACGGCGAATCTTCCCa < 1:2824611/65‑1 (MQ=255) gagcGGCCTTTTCCGGCGTCTCAACCCCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCa < 1:30333052/64‑1 (MQ=255) gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTccc > 1:25640642/1‑66 (MQ=255) aTCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCCCt > 1:7773174/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:35802160/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:19011610/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACtt > 1:33838179/1‑68 (MQ=255) tCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACAGTGCGCCACGGCGAATCTTCCCACtt > 1:31797861/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg > 1:30090542/1‑68 (MQ=255) gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg > 1:7174882/1‑68 (MQ=255) ctttttCCGGCGTCCCACCCCCCGGCGCGGGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCg < 1:36785481/65‑1 (MQ=255) ccTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCgg > 1:25344210/1‑68 (MQ=255) ccTTTGCCGGCGGCTCACCCCCCGGCGGGGGGCAAACGGGGCCCCACGGCGAATCTTCCCACTTGCg < 1:18364880/67‑1 (MQ=255) ttttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTc > 1:14055606/1‑68 (MQ=255) cgggCGTCTCAACCCCCGGCGCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg < 1:28642654/66‑1 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:2963941/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:27673735/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:33034866/1‑68 (MQ=255) ccGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgg > 1:9786206/1‑68 (MQ=255) gggCGTCTCACCACCGGGCGCGGGGTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:36003835/67‑1 (MQ=255) cGGCGTCTCACCCCCGGGCGCGCGTTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg < 1:18313720/68‑1 (MQ=255) ggcggcTCAGCACCCGGCGCGCGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:26037664/68‑1 (MQ=255) ggCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:6642137/1‑67 (MQ=255) ggCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACggg > 1:19905144/1‑67 (MQ=255) ggCGTCTCACCCCCCGGCGCGCGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACgggg < 1:11814679/68‑1 (MQ=255) ctcATCACCCGGCGCGGGGTAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:18040247/67‑1 (MQ=255) ctcATCACCCGGCGCGCGGGAAACCGTGGGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:7373420/67‑1 (MQ=255) ctcAGCACCCGGCGCGCGGTAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGa < 1:2119019/67‑1 (MQ=255) ctcACCCCCCGGCGCGCGGAAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc < 1:1954541/68‑1 (MQ=255) atccCCCGGCGCGCGGCAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:20208600/68‑1 (MQ=255) atcaCCGGGCGGCCGGGAAACCGGGGGCCACGGCTAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:9169978/68‑1 (MQ=255) accaCCCGGCGCGCGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGActct < 1:5851588/66‑1 (MQ=255) tcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGAc > 1:8807406/1‑64 (MQ=255) tcaCCCGGCGCGCGGCAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTg < 1:34717995/68‑1 (MQ=255) cccccGGCGGGCGGTAACCGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:12624398/66‑1 (MQ=255) cccccGGCGGGCGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:18095608/66‑1 (MQ=255) cccccGGCGCGGGGTAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:13098669/66‑1 (MQ=255) caCCCGGCGCGGGGGACACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:15616666/68‑1 (MQ=255) caCCCGGCGCGGGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGt < 1:26585797/68‑1 (MQ=255) aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:29338986/1‑68 (MQ=255) aCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGtt > 1:16041452/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:13406712/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:5584871/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:30527179/1‑68 (MQ=255) cccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGttt > 1:6391651/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:24638746/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:29933399/1‑68 (MQ=255) ccGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:29042271/1‑68 (MQ=255) cGGCGCGCGGGAAACGGGCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGt < 1:20875981/68‑1 (MQ=255) cGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTg > 1:16411280/1‑67 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg > 1:30599321/1‑68 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg > 1:8228822/1‑68 (MQ=255) aaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgg > 1:22346981/1‑68 (MQ=255) aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCgcgggcgtg > 1:12110838/1‑66 (MQ=255) aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg > 1:21555038/1‑68 (MQ=255) aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGcgggcggg > 1:1058403/1‑68 (MQ=255) | AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGG > CP009273/2711607‑2711740 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |