| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,762,278 | G→A | 13.1% | L85L (CTG→CTA) ‡ | yfjQ → | CP4‑57 prophage; uncharacterized protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,762,278 | 0 | G | A | 13.1% | 1214.5 / 154.7 | 442 | L85L (CTG→CTA) ‡ | yfjQ | CP4‑57 prophage; uncharacterized protein |
| Reads supporting (aligned to +/- strand): ref base G (246/138); new base A (48/10); total (294/148) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01 | |||||||||||
Only 100 of 551 total aligned reads displayed.
GCCATTCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACG > CP009273/2762212‑2762345 | tCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCt > 1:1025966/1‑67 (MQ=255) tCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTa < 1:31479535/68‑1 (MQ=255) cTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTAc < 1:27449612/68‑1 (MQ=255) cTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTAc < 1:21916314/68‑1 (MQ=255) tttGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACg < 1:29197408/68‑1 (MQ=255) tttGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCCCCTACg > 1:20940317/1‑68 (MQ=255) ccTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCgg > 1:15218558/1‑68 (MQ=255) cTGTCAGACACGGGTACGTGACCCCCGTCCTCGTGAGCATACTAAGCATATCCTGCGCCTACGTCgg < 1:34738044/67‑1 (MQ=255) cacGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAgggg < 1:12994709/65‑1 (MQ=255) cacGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaa > 1:21497031/1‑68 (MQ=255) cacGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaa > 1:16670257/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa < 1:10078144/68‑1 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:8967265/1‑68 (MQ=255) acGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCaaa > 1:23352254/1‑68 (MQ=255) ggTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAAtt > 1:965517/1‑67 (MQ=255) ggTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAAtt > 1:12700037/1‑67 (MQ=255) gTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTa > 1:28919407/1‑67 (MQ=255) tACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTAcc > 1:20827545/1‑68 (MQ=255) aCGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTAcc < 1:35248502/67‑1 (MQ=255) cGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCgg < 1:7762291/68‑1 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt < 1:10883701/68‑1 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt > 1:14388452/1‑68 (MQ=255) gTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGt > 1:19987496/1‑68 (MQ=255) tGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTa > 1:36864570/1‑68 (MQ=255) tGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTa > 1:35601570/1‑68 (MQ=255) gACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:6096671/1‑68 (MQ=255) gACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:7464413/1‑68 (MQ=255) gACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:6828903/1‑68 (MQ=255) aCCCCCGTCGTCGTGAGCATACTAAGCATATGGTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:24818130/1‑67 (MQ=255) aCCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTaa > 1:7003291/1‑67 (MQ=255) cccGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAg > 1:29984880/1‑68 (MQ=255) ccGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAgg < 1:20593609/68‑1 (MQ=255) tcgtcgtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGtt > 1:28027358/1‑67 (MQ=255) cgtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGa < 1:25568601/68‑1 (MQ=255) gtGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGaa < 1:25292721/68‑1 (MQ=255) tGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCATATTACCGGTAAACAGGTTCCGGaa < 1:13601350/67‑1 (MQ=255) tcATACTAAGCATATGCTGCGCCTACGTCGGGAGTGGCAAATTACCGGTAAACAGGTTCCGGAAAtt < 1:36388259/66‑1 (MQ=255) gCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAAtt < 1:25854650/67‑1 (MQ=255) gCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTa > 1:16827318/1‑68 (MQ=255) gCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTa > 1:33867895/1‑68 (MQ=255) aTACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt < 1:36616487/67‑1 (MQ=255) tACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:4417212/1‑66 (MQ=255) aCTAAGCATATGCTGCGCCTACGTCGGGTGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCt > 1:24138420/1‑68 (MQ=255) aCTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTAt > 1:36081081/1‑65 (MQ=255) cTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCt > 1:17186273/1‑67 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCt > 1:13334363/1‑66 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:32255920/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:34167215/68‑1 (MQ=255) tAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGc < 1:26802737/68‑1 (MQ=255) aaacATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGc < 1:10650072/64‑1 (MQ=255) aaacATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGc < 1:3939147/64‑1 (MQ=255) aaacATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGc < 1:23548057/64‑1 (MQ=255) aaacATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCt > 1:33436514/4‑68 (MQ=255) aaacATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCt > 1:18146492/4‑68 (MQ=255) aaGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCt > 1:22257882/1‑68 (MQ=255) aaGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCt > 1:30853483/1‑68 (MQ=255) gCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:32782061/1‑68 (MQ=255) gCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:27072766/1‑68 (MQ=255) acATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTa > 1:28461694/2‑68 (MQ=255) acATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTa > 1:5042458/2‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:4716601/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:33582676/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:35979772/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:14660618/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:2501108/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:18397998/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:1628436/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:8358526/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:32363425/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTTaa > 1:244936/1‑68 (MQ=255) cATATGTTACGTCTTCGCAGGGAAGGGCATATTAACGGGCAGGAAGTTCCTGAAATTATCCTGCTCaa > 1:33178475/1‑68 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:14947450/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:13176436/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:37121378/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:19400578/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:8560515/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:31718595/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:22427002/1‑67 (MQ=255) cATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:23086310/1‑67 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:12184708/1‑66 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:34992402/1‑66 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTa > 1:1908363/1‑66 (MQ=255) atatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCAGCTTa > 1:1963288/1‑66 (MQ=255) aatGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTCCTTAAc > 1:29423744/2‑67 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:938151/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:11084528/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:26430725/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:23863279/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:555491/1‑68 (MQ=255) gctgcGCCTACGTCGGGAGGGGCAAAATACCGGAAAACCGGTTCCGGAAATTAACCTGCTTAActcac > 1:5735920/1‑66 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:15945357/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:15596212/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:1772216/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:2461882/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:18852042/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:29572030/1‑67 (MQ=255) ctgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACtctc > 1:22851683/1‑67 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCAc > 1:6315253/1‑68 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCAc > 1:2018962/1‑68 (MQ=255) tgcGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCAc > 1:21800946/1‑68 (MQ=255) | GCCATTCTTTGCCTGTCAGACACGGGTACGTGACCCCCGTCGTCGTGAGCATACTAAGCATATGCTGCGCCTACGTCGGGAGGGGCAAATTACCGGTAAACAGGTTCCGGAAATTATCCTGCTTAACTCTCACG > CP009273/2762212‑2762345 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |