| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,580 | T→C | 5.9% | A133A (GCT→GCC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,580 | 0 | T | C | 5.9% | 628.2 / 11.2 | 206 | A133A (GCT→GCC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base T (107/78); new base C (4/8); total (111/95) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 206 total aligned reads displayed.
GGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGC > CP009273/122514‑122646 | gATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCATGGTGAAAGTTGGCGAAAAAGTTGCAGCTGa > 1:31491975/1‑68 (MQ=18) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:3623630/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:21081213/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:33145261/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGa > 1:27187681/1‑67 (MQ=255) atatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGAGAAAGTTGCCGCTGa > 1:37024055/1‑67 (MQ=18) tatCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAc > 1:28713128/1‑68 (MQ=255) atCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa < 1:21563055/68‑1 (MQ=255) atCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACa > 1:15241560/1‑68 (MQ=255) cGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGt < 1:7287623/68‑1 (MQ=255) ggCTGCGACGAAGTTGAAGTGACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc < 1:14819889/68‑1 (MQ=255) ggCAGCGACGAACTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAAAAGTc < 1:35892568/68‑1 (MQ=255) gCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:18214240/1‑67 (MQ=255) gCAGCGACGAAGTTGAAGTGACAGAAGTCCTGGTGAAAGTTGGCGAAAAAGTTGCCGCTGAACAGTc > 1:22646236/1‑67 (MQ=14) tgtgacgaAGTTGAACTGACCGATATCCTGGTGAAAGTTGGCGACAAAGTTGAAGCTGAACAGTCGc < 1:30110019/64‑1 (MQ=17) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:18612695/1‑65 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:25362472/1‑65 (MQ=255) aGCGACGAAGTTGAAGTGACCGAAATCCTGGCGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTc > 1:24338047/1‑65 (MQ=255) gCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTg > 1:20541741/1‑68 (MQ=255) cgatgaAGTTGAACTCACCGACATCCTGGTGAAAGTTGGCGAAAAAGTTGAAGCTGAACAGTCGCTGa < 1:19511932/68‑1 (MQ=11) gatgaAGTTGAACTCACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa < 1:35568199/64‑1 (MQ=14) gatgaAGTTGAACTCACCGACATCCTGGTCAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa < 1:33668948/64‑1 (MQ=1) gacgaAGTTGAAGTGACCGAAATCCTGGGGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCt < 1:34741236/65‑1 (MQ=255) gacgaAGTTGAACTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGa < 1:34422065/67‑1 (MQ=255) acgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAATCTGAACAGTCGCTGATc > 1:7795829/1‑68 (MQ=255) tgaAGTTGAACTGACCGACATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:13416356/66‑1 (MQ=17) tgaAGTTGAACTCACCGACATCCTGGTCAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:12451725/66‑1 (MQ=11) cgaAGTTGAAGTGACCGAGATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:31933409/67‑1 (MQ=255) cgaAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATc < 1:30456888/67‑1 (MQ=255) atgTTGAAGTGACCGAAATGCTGGTGAAAGTTGGCGATAAAGTTGCAGCTGAACAGTCTCTGATCAcc < 1:11339461/66‑1 (MQ=255) aGTTGAATTGACCGATATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:32376783/67‑1 (MQ=255) aGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:37015427/67‑1 (MQ=255) aGTTGAACTGACCGATATCCTGGTCAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCAcc < 1:32443353/67‑1 (MQ=14) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGaa > 1:4299392/1‑67 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg < 1:6930951/68‑1 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:7340217/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:13596498/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:29128728/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:14939065/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:33290996/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:19821577/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:17825253/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:4793272/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg > 1:4485381/1‑68 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAg < 1:16610107/68‑1 (MQ=255) aGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCACTGATCACCGTa > 1:31242749/1‑64 (MQ=255) tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGc > 1:2687040/1‑68 (MQ=255) tGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCACTGATCAc > 1:7217885/1‑58 (MQ=255) gACCGAAATCCTGGTGAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCg < 1:1447339/68‑1 (MQ=255) cGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACa < 1:29825033/68‑1 (MQ=255) taTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:2444329/67‑1 (MQ=255) aaTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:17136791/68‑1 (MQ=255) aaTACTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAgg < 1:24767043/68‑1 (MQ=255) aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTa > 1:30897709/1‑54 (MQ=255) aTCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGc < 1:8334489/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:163290/1‑68 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:30074420/1‑68 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:5120735/68‑1 (MQ=255) tCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAAGCt > 1:9023418/1‑68 (MQ=255) tCCTGGTGAAAGTGGGCGATAAAGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt < 1:32609570/68‑1 (MQ=255) ccTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCt > 1:9777377/1‑67 (MQ=255) ccTGGTCAAAGTTGGCGATAAAGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt < 1:7507772/68‑1 (MQ=255) cTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGGCGCTGATCACCGTAGAAGGCGACAAGGCTTc < 1:9308790/68‑1 (MQ=255) cTGGTCAAAGTGGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTc < 1:2555407/68‑1 (MQ=255) tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:13951423/68‑1 (MQ=255) tGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:9652075/68‑1 (MQ=255) tGGTCAAAGTGGGCGAAAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCt < 1:6502706/68‑1 (MQ=11) ggTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTa > 1:1060456/1‑68 (MQ=255) tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg > 1:23430053/1‑68 (MQ=255) tGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg > 1:20865011/1‑68 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATgg > 1:35026754/1‑68 (MQ=255) gAAAGTTGGCGATAAAGTTGAAGCAGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATg < 1:1678343/67‑1 (MQ=255) aGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCtt > 1:25178841/1‑59 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:15351573/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:4966472/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:18835795/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:7862811/1‑68 (MQ=255) gTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGt > 1:22814253/1‑68 (MQ=255) gggCGAGAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGtt < 1:23993174/66‑1 (MQ=255) gggCGACAAAGTTGAAGCTGAACAGTCGCTGATCCCCGTAGAAGGCGACAAGGCTTCTATGGAAGtt < 1:12909042/66‑1 (MQ=14) gATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGc < 1:31497830/68‑1 (MQ=255) tAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTc < 1:13195809/68‑1 (MQ=255) aaaGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:19960922/68‑1 (MQ=255) aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc < 1:36564388/68‑1 (MQ=255) aaaGTTGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTcc > 1:36990432/1‑68 (MQ=255) aaGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGGCAAGGCTTCTATGGAAGTTCCGGCTCCg < 1:33231817/68‑1 (MQ=14) aaGTTGAAGCGGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCg < 1:24111778/68‑1 (MQ=255) gTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt > 1:34657053/1‑68 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGttt > 1:26045751/1‑67 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt < 1:1001391/66‑1 (MQ=255) tGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:36790811/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGtt < 1:13148672/66‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:15704362/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:23897690/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTg < 1:33099990/68‑1 (MQ=255) tGAAGCCGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCGTCTATGGAAGTTCCGGCTCCGttt > 1:16486487/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGcag > 1:11141367/1‑65 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTg > 1:20755504/1‑67 (MQ=255) gCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCGCCGTTTGCTg > 1:36080421/1‑67 (MQ=255) cTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGc > 1:6868212/1‑68 (MQ=255) | GGATATCGGCAGCGACGAAGTTGAAGTGACCGAAATCCTGGTGAAAGTTGGCGATAAAGTTGAAGCTGAACAGTCGCTGATCACCGTAGAAGGCGACAAGGCTTCTATGGAAGTTCCGGCTCCGTTTGCTGGC > CP009273/122514‑122646 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |