| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 122,853 | G→C | 5.2% | V224V (GTG→GTC) | aceF → | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 122,853 | 0 | G | C | 5.2% | 1276.2 / 4.7 | 379 | V224V (GTG→GTC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
| Reads supporting (aligned to +/- strand): ref base G (167/188); new base C (3/19); total (172/207) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 396 total aligned reads displayed.
CAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTT > CP009273/122786‑122920 | cGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATg > 1:34359363/1‑68 (MQ=255) cGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATg > 1:21173574/1‑68 (MQ=255) cGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGAGATg > 1:34698005/1‑68 (MQ=255) ggCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGa < 1:35381126/65‑1 (MQ=255) ggCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGa < 1:17551645/65‑1 (MQ=255) ggCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATgg < 1:26387385/68‑1 (MQ=255) ggCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATgg < 1:17086979/68‑1 (MQ=255) ggCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATg < 1:22153446/67‑1 (MQ=255) cTGGCGTGAAAGAAGTTAACGTTCGGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGt < 1:9831213/67‑1 (MQ=255) cTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTg > 1:6168842/1‑68 (MQ=255) tGGGGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGa < 1:10533975/68‑1 (MQ=255) gCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGaaa > 1:25345409/1‑68 (MQ=255) cGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAg > 1:28919461/1‑68 (MQ=255) taaaGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTgg < 1:31783000/67‑1 (MQ=255) gAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTgg < 1:20249930/68‑1 (MQ=255) aagaagTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGc < 1:3844747/68‑1 (MQ=255) agaagTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCg > 1:13468390/1‑68 (MQ=255) agaagTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCg > 1:7800706/1‑68 (MQ=255) agaagTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCg > 1:22507803/1‑68 (MQ=255) acgTTAACGTTCCGGATATCGGCAGCGACGAAGTTGAAGTGACTGAAGTCATGGTGAAAGTGGGCGaa > 1:16607590/3‑67 (MQ=11) agTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACa > 1:30505720/1‑68 (MQ=255) ttAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACaaa > 1:5822673/1‑68 (MQ=255) cGTTCCGTATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGAGACAGtt > 1:34617733/1‑67 (MQ=21) cGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGtt > 1:13028258/1‑67 (MQ=255) gTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTg > 1:11312254/1‑67 (MQ=255) gTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTg > 1:35661838/1‑67 (MQ=255) accGGACATCGGCGCTGACGAAGTTGAACTGACTGACCTGATGGTGAAAGTGGGCGACAAAGTTGcc < 1:30168602/66‑1 (MQ=18) accGGAAATCGGCGGTGACGAAGTTGAAGTGACTGATTTCATGGTCAAAGTGGGCGACAAAGTTGcc < 1:5418886/66‑1 (MQ=18) accGGAAATCGGCGGTGACGAAGTTGAAGTGACAGAACTGATGGTGAAAGTGGGCGACAAAGTTGcc < 1:18121996/66‑1 (MQ=21) ccGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGc < 1:21043498/68‑1 (MQ=255) tatCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAAc < 1:6940724/68‑1 (MQ=255) tatCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAAc > 1:2855599/1‑68 (MQ=255) tatCGGCGGCGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGaa < 1:13605070/67‑1 (MQ=255) aatCGGGGCTGACGAAGTTGAATTGACCGAACTGATGGTCAAAGTGGGCGACAAAGTTGCCGCTGaa < 1:26678892/66‑1 (MQ=17) atCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACa < 1:7339005/68‑1 (MQ=255) cggcggTGACGAAGTTGAAGTGTCAGAAGTGATGGTGAAAGTTGGCGACAAAGTTGCCGCTGAACAGt < 1:32458077/68‑1 (MQ=21) cggcagCGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGt < 1:9299657/68‑1 (MQ=255) gtcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:33259459/66‑1 (MQ=255) gggggTGACGAAGTTGAACTGACGGATCTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:8515615/65‑1 (MQ=21) gggggTGACGAAGTTGAACTGACCGACCTCCTGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:10242218/65‑1 (MQ=14) gggggTGAAGAAGTTGAACTCACCGACTTCCTGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:34008717/65‑1 (MQ=0) ggctgTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:11070673/68‑1 (MQ=255) ggcggcGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:21634404/68‑1 (MQ=255) ggcggcGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:30855151/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc > 1:32535797/1‑68 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:4309735/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:26914877/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:10077145/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:26464358/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc > 1:20707284/1‑68 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:21298679/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:21449123/68‑1 (MQ=255) ggcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:14094433/68‑1 (MQ=255) ggccgTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc < 1:35782496/68‑1 (MQ=255) gcggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc > 1:15185054/1‑67 (MQ=255) ggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTc > 1:274848/1‑65 (MQ=255) ggTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACt < 1:24713217/68‑1 (MQ=255) gTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTg > 1:23598677/1‑68 (MQ=255) gACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGa < 1:18611675/67‑1 (MQ=255) gACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGa < 1:8194368/67‑1 (MQ=255) ctaaGTTGAAGTGACTGACTTGAGGGGGATAGTGGTCGACAAACTTGCCGATGAACAGTCACTGATc < 1:299303/65‑1 (MQ=255) cGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATc < 1:28346772/67‑1 (MQ=255) cGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATc < 1:1224646/67‑1 (MQ=255) cGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATc < 1:1968129/67‑1 (MQ=255) cGAAGTTGAACTGACGGATATCATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATc < 1:3603741/67‑1 (MQ=18) aGTTGAATTCACCGATATGCTGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:11918865/67‑1 (MQ=14) aGTTGAAGTGACTGAATTCATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:27921374/67‑1 (MQ=255) aGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:9433239/67‑1 (MQ=255) aGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:24912909/67‑1 (MQ=255) aGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:33532752/67‑1 (MQ=255) aGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCAcc < 1:4568676/67‑1 (MQ=255) ttGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTa > 1:6530058/1‑68 (MQ=255) ttGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTa > 1:19277439/1‑68 (MQ=255) tGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGt < 1:17692659/66‑1 (MQ=255) aGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTTATCACCGTAGAAg > 1:35039620/1‑68 (MQ=255) aGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGa > 1:25898472/1‑66 (MQ=255) aGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAg > 1:20550559/1‑68 (MQ=255) aGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAg > 1:1601749/1‑68 (MQ=255) aGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAg > 1:9655872/1‑68 (MQ=255) tGACTGAAGTGATGGTGAAAGTGGGCGACTAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGc > 1:22380878/1‑68 (MQ=255) tGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGc > 1:13916317/1‑68 (MQ=255) tGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGc > 1:3697131/1‑68 (MQ=255) tGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGc > 1:36557630/1‑68 (MQ=255) tGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGc > 1:1989422/1‑68 (MQ=255) gACTGAAGTGATGGTGAAAGTTGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCg < 1:26162072/68‑1 (MQ=255) gACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCg < 1:18803953/68‑1 (MQ=255) aGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa > 1:1566413/1‑67 (MQ=255) aGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa > 1:14814026/1‑67 (MQ=255) gTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa > 1:500171/1‑66 (MQ=255) gTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa > 1:31576148/1‑66 (MQ=255) gTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa > 1:23248287/1‑66 (MQ=255) gTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACaaa > 1:24116703/1‑66 (MQ=255) gTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAga > 1:4087990/1‑67 (MQ=255) tGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt > 1:2939736/1‑68 (MQ=255) gATGGTGAAAGTTGGCGACAAAGTTGGCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt < 1:10746264/67‑1 (MQ=255) gATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCtt < 1:2277858/68‑1 (MQ=255) gATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt > 1:32902435/1‑67 (MQ=255) gATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt > 1:1627837/1‑67 (MQ=255) gATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt > 1:23464419/1‑67 (MQ=255) gATGGAGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCt < 1:25744172/67‑1 (MQ=255) | CAGCGGCTGGCGTGAAAGAAGTTAACGTTCCGGATATCGGCGGTGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGACAAAGTTGCCGCTGAACAGTCACTGATCACCGTAGAAGGCGACAAAGCTT > CP009273/122786‑122920 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |