| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,447,085 | C→T | 30.6% | A68T (GCT→ACT) | gspB ← | part of gsp divergon involved in type II protein secretion |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,447,085 | 0 | C | T | 30.6% | 529.9 / inf | 392 | A68T (GCT→ACT) | gspB | part of gsp divergon involved in type II protein secretion |
| Reads supporting (aligned to +/- strand): ref base C (74/196); new base T (35/84); total (110/282) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
Only 100 of 425 total aligned reads displayed.
CGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCACCAACCAGC > CP009273/3447019‑3447152 | cgacgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGcc < 1:33741788/68‑1 (MQ=255)cgacgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGcc < 1:18781740/68‑1 (MQ=255)cgacgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGcc < 1:21593358/68‑1 (MQ=255) cgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGtctc < 1:31291871/67‑1 (MQ=255) cgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTctct < 1:7863808/68‑1 (MQ=255) cgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCctct < 1:16893963/68‑1 (MQ=255) cgGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCctc < 1:35341374/67‑1 (MQ=255) ggggAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTa < 1:25257593/68‑1 (MQ=255) ggAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTAc < 1:4454912/67‑1 (MQ=255) ggAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTAc < 1:6286005/67‑1 (MQ=255) gTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTg > 1:3690158/1‑68 (MQ=255) gTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTg > 1:22966184/1‑68 (MQ=255) ggCTGTTTTTTGTAGAAATTGTGTTGTGTTGATTGTTTCAAAGCGGGCGTTTCAGCCTCTACAGTGa < 1:35882801/67‑1 (MQ=255) ggCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGa < 1:2645752/67‑1 (MQ=255) ggCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGa < 1:4899193/67‑1 (MQ=255) tGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTc > 1:29604207/1‑68 (MQ=255) tGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTc > 1:30339852/1‑68 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCgg < 1:23235103/67‑1 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCgg < 1:31588913/67‑1 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCgg < 1:17647871/67‑1 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCgg < 1:11753913/67‑1 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCgg < 1:18928098/67‑1 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCgg < 1:7770720/67‑1 (MQ=255) tttttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCgg < 1:5986838/67‑1 (MQ=255) tttGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCtt > 1:30612432/1‑68 (MQ=255) tGAAGAAATAGTGTTGTGTTGATTGTTTAAACGCGGGCGTTTCAGGCTCTACAGTGACTTCGGCtttt < 1:8961702/68‑1 (MQ=255) tGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCtttt < 1:26032317/68‑1 (MQ=255) tGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCtttt < 1:34972399/68‑1 (MQ=255) tGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCtttt < 1:34210406/68‑1 (MQ=255) tGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCtttt < 1:17488669/68‑1 (MQ=255) tGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCtttt < 1:5131674/68‑1 (MQ=255) tGAAGAAATAGTGTGGTGTTGATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCtttt < 1:20781553/68‑1 (MQ=255) agaaATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTa > 1:25923775/1‑66 (MQ=255) agaaATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGACTCTACATTGACTTCGGCTTTTACt > 1:4147564/1‑68 (MQ=255) aaTAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTAt < 1:27109330/67‑1 (MQ=255) aaTAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTAt < 1:13434069/67‑1 (MQ=255) tAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCa > 1:29276184/1‑68 (MQ=255) aGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCaa < 1:24276507/68‑1 (MQ=255) tgttgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCaaa > 1:2388062/1‑67 (MQ=255) gttgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGc > 1:1006385/1‑68 (MQ=255) gttgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGc > 1:4360111/1‑68 (MQ=255) tgtgtttATTGTTTAAAAGCGTTGGTTTCAGTCTCTACAGTGTGTTCGTGTTTTACTTTCCAATGCAc < 1:26715876/68‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTTAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAc < 1:8280850/68‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCa < 1:29143794/67‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAgaa > 1:14550317/1‑65 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCa > 1:18306849/1‑67 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCa > 1:20433667/1‑67 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAc < 1:15644814/68‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAc < 1:22668255/68‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAc < 1:14300651/68‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAc < 1:21053361/68‑1 (MQ=255) tgtgttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAc < 1:14011211/68‑1 (MQ=255) gttgATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCAccaa > 1:24008324/1‑66 (MQ=255) gttgATTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCa > 1:35388940/1‑68 (MQ=255) gttgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCa > 1:8642134/1‑68 (MQ=255) tgATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATa > 1:32355949/1‑68 (MQ=255) tgATTGTGTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATa < 1:16520059/68‑1 (MQ=255) aTTGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATa > 1:27436819/1‑66 (MQ=255) ttGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATa > 1:11213456/1‑65 (MQ=255) ttGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATa > 1:31740160/1‑65 (MQ=255) ttGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATa > 1:26891514/1‑65 (MQ=255) ttGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGc < 1:16734190/67‑1 (MQ=255) tGTTTAAAAGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTg < 1:1934968/68‑1 (MQ=255) tGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTg < 1:33216876/68‑1 (MQ=255) gTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGc < 1:5638350/68‑1 (MQ=255) gTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGc < 1:18251666/68‑1 (MQ=255) ttAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCt < 1:28839135/68‑1 (MQ=255) ttAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCt < 1:32005390/68‑1 (MQ=255) ttAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCt < 1:15358484/68‑1 (MQ=255) aaaaGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTgg < 1:34511929/68‑1 (MQ=255) aaaaGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTgg < 1:17239690/68‑1 (MQ=255) aaaaGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTgg < 1:1296240/68‑1 (MQ=255) aaaaGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTgg < 1:19466415/68‑1 (MQ=255) aaaGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGc < 1:25408082/68‑1 (MQ=255) aaGCGGGCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCa < 1:3652235/68‑1 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:7061780/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:7562820/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:10296278/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:30006581/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:14924772/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:23469775/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:158778/1‑68 (MQ=255) aGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCAt > 1:1849167/1‑68 (MQ=255) gggCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAcc < 1:12243945/68‑1 (MQ=255) gCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAccc < 1:32860596/67‑1 (MQ=255) gCGTTTCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAccc < 1:24612025/67‑1 (MQ=255) gCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAccc < 1:35544212/67‑1 (MQ=255) gCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAccc < 1:14763460/67‑1 (MQ=255) gCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATAccc < 1:22019965/67‑1 (MQ=255) ttCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:29692260/68‑1 (MQ=255) ttCAGTCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:1980854/68‑1 (MQ=255) ttCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:9375607/68‑1 (MQ=255) ttCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:10698598/68‑1 (MQ=255) ttCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:28589842/68‑1 (MQ=255) ttCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:29040191/68‑1 (MQ=255) ttCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaa < 1:33505096/68‑1 (MQ=255) tCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCaccaac < 1:28118350/68‑1 (MQ=255) ccTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCACCAACCAGc > 1:19119459/1‑68 (MQ=255) ccTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCACCAACCAGc > 1:19613465/1‑68 (MQ=255) ccTCTACAGTGACTTAGGCTTTTACTATCCAAAGCACCCATACCTGCCTGGCATACCCaccaacctg > 1:35087331/1‑65 (MQ=255) | CGACGGGGAGTGGCTGTTTTTTGAAGAAATAGTGTTGTGTTGATTGTTTAAAAGCGGGCGTTTCAGCCTCTACAGTGACTTCGGCTTTTACTATCCAAAGCACCCATAGCTGCCTGGCATACCCACCAACCAGC > CP009273/3447019‑3447152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |