| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,400 | A→G | 5.5% | S271S (AGT→AGC) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,400 | 0 | A | G | 5.5% | 1416.1 / 23.6 | 455 | S271S (AGT→AGC) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (240/179); new base G (19/6); total (270/185) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.31e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 459 total aligned reads displayed.
AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 | aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTa < 1:36130559/65‑1 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTg > 1:10187359/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:27556388/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:556394/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:25107684/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:33778623/1‑68 (MQ=255)aGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTg > 1:13995286/1‑68 (MQ=255) cAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTgg < 1:31726045/66‑1 (MQ=255) gggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATc > 1:7181589/1‑68 (MQ=255) ggTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCg > 1:26472025/1‑68 (MQ=255) gTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTCCTGGCATcat > 1:29945842/1‑66 (MQ=255) ttACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGt < 1:14244381/67‑1 (MQ=255) ttACCAAACTGACTGAATTCTGTTGCCGTTGCCGCGCGCACATAACGCCATGGGTTACTGGCATCGt < 1:35925156/67‑1 (MQ=25) aCCATACTGGCTGATTTCTGTTGCCGTTGCCGCTCGAACATAACGCCATGGGTTGCTGGCATCAttgc < 1:18297425/68‑3 (MQ=17) ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCGtttga > 1:16366969/1‑66 (MQ=255) ccATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGtttgg > 1:29621296/1‑66 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACTTAACGCCATGGGTTACTGGCATCGTtttct < 1:21511331/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCt > 1:22232685/1‑55 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCAtttatt > 1:23941952/1‑65 (MQ=18) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttttgt > 1:4018660/1‑66 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGttttat > 1:10724997/1‑66 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:4844261/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:33102996/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:21815700/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:3765092/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct > 1:17792323/1‑68 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttct < 1:14688904/68‑1 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCAtttatt > 1:24407210/1‑65 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCAtttagt > 1:13441591/1‑65 (MQ=255) cATACTGGCTGATTTCTGTTGCCGTAGCCGCTCGAACATAACGCCATGGGTTGCTGGc < 1:23620157/58‑1 (MQ=17) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttgtt > 1:26945244/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:12013521/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:15132780/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:22988354/1‑67 (MQ=255) tACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctt > 1:31360542/1‑67 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATtttgtttc > 1:17929904/1‑68 (MQ=18) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTtttctttc > 1:924950/1‑68 (MQ=255) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCATttattttc > 1:28429600/1‑68 (MQ=18) aCTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCAAGGGTTACTGGCATCGTtttctttc > 1:11145895/1‑68 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATCACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:15612995/68‑1 (MQ=255) cTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCg < 1:15808325/68‑1 (MQ=255) ggCTGATTTCTGTTGCAGTTGCCGCGCGAACATATCGCCATGGGTTACTGGCATCTTTTCGTTTCGCa > 1:36693627/1‑68 (MQ=255) cTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCTTTTGCTTTCGctta > 1:27234559/1‑65 (MQ=21) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATc < 1:36495436/67‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:32068928/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:12852694/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:20939742/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCg < 1:20488638/68‑1 (MQ=255) tGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGCTTACTGGCATCGTTTTCTTTCGCATCg < 1:30129355/68‑1 (MQ=255) ttCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTGCTTTCGCATCTccgg > 1:19450996/1‑66 (MQ=21) tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc < 1:34504725/68‑1 (MQ=255) tGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGc < 1:8713899/68‑1 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa > 1:32722504/1‑68 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCa > 1:29257014/1‑68 (MQ=255) gttgccgttgccGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCa > 1:3296620/1‑68 (MQ=255) agccgtcgccgCTCGACCATAACGCCATGGGGTACTGGCATCGGTTTCTTACGCATCGCCTGGGCAAt < 1:13817896/67‑1 (MQ=25) cgttgccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCGGGCCAGttt > 1:33370271/1‑67 (MQ=25) gccgCGCGAACATAACGCCTTGGGGTTATGGCATGCTAGTCGATCGCAACGCGTGGGCGATTTGCcgc > 1:2272906/1‑68 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:2607973/68‑1 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc < 1:17064445/68‑1 (MQ=255) gccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc > 1:25823855/1‑68 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:21344599/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCAGCGTTTTCTTTCGCATCGCCTGGGCATTTTGCcgcga > 1:32321025/1‑67 (MQ=38) gctcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:5824018/65‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:9432161/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:12188939/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:33641963/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:4235677/68‑1 (MQ=255) gcgAACTTCACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCt < 1:35808537/67‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTa < 1:16590580/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:7838223/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:30735574/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:7507576/68‑1 (MQ=255) aCATAACGCGAGGGGTTACCGGCAGCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACcca < 1:3704717/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAgg > 1:6702083/1‑67 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:25518158/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc < 1:21076343/68‑1 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGc > 1:23228213/1‑68 (MQ=255) ccATGGGTTACTGGCATCTTTTTGTTTCGCATCGCCAGGGCAGTTAGAAGAGCCTACCCACCAGGcat > 1:19738623/1‑66 (MQ=2) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:5159550/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:7308619/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:9680845/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCCGCTACCCACCAGGcat > 1:6257018/1‑66 (MQ=21) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAGTTTGCCGCGCCTACCCACCAGGcat > 1:30825514/1‑66 (MQ=37) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCAGGGCAATTTGCCGCGGCTACCCACCAGGctt > 1:8615341/1‑66 (MQ=37) ccATGGGTTACTGGCATCGTTTACTTTCGCATCGCCAGGCCAATTTGAAGCGCCTACCCACCAGGcat > 1:17397708/1‑66 (MQ=18) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:28041466/68‑1 (MQ=255) cATGGGTTACTGGCATCGTTTTCTTTCGCATAGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt > 1:24862091/1‑68 (MQ=255) cATGGGTTACTGGACTCGTCTGCTATCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGCGtt < 1:8332080/68‑1 (MQ=255) cATGGGTTACTGGAATCTTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGtt < 1:18918890/68‑1 (MQ=255) cATGGGTTAATGGACTGTTTATCTCTCGCATCGCCTGGGCAATTTGCCGCGCCAACCCACCAGGCGt < 1:30622625/67‑1 (MQ=255) aTGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGCGCCTACCCACCAGGCCttt > 1:11404475/1‑67 (MQ=38) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:14827793/68‑1 (MQ=255) cgggTTACTGGCAGCGTTTTCTTTCGCATCTCGTGCGCAATTTTCAGCGCCAACCCACCAGGCGTTCt < 1:19975427/67‑1 (MQ=21) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:28695939/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:34765705/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:13249729/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAATTTGCCGCGCCTACCCACCAGGCGTTTTta > 1:28901206/1‑68 (MQ=255) gTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta < 1:30427847/67‑1 (MQ=255) gTTACTGGCATCGTTATCTATCGCATCGCCTGGGCAATATGCCGCGCCTACCCACCAGGCGTTCTta < 1:28567644/67‑1 (MQ=255) | AGCCAGGGTTACCATACTGGCTGATTTCTGTTGCCGTTGCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAA > CP009273/3462336‑3462467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |