| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,438 | C→A | 7.0% | A259S (GCA→TCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,438 | 0 | C | A | 7.0% | 1952.6 / 12.3 | 668 | A259S (GCA→TCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (163/434); new base A (19/26); total (182/486) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.93e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 668 total aligned reads displayed.
GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAC > CP009273/3462374‑3462503 | cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg > 1:29606939/1‑67 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTGGTTTCGCATCGCCCGGGCATTTTGCagcg > 1:7981703/1‑67 (MQ=21) gctcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:5824018/65‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:35205627/68‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:9432161/68‑1 (MQ=255) gcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCt < 1:25295996/67‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:7507576/68‑1 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacc > 1:11447996/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:31065911/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:11136091/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCg > 1:26811613/1‑68 (MQ=255) ccATGGGTTTCTGGCATCATTTATTTTAGCATCGCCAGGACAATTAGCCGCGCCTACCCACCAGGcat > 1:479495/1‑66 (MQ=9) ccATGGGTTACTGGCATCTTTTTGTTTCGCATCGCCAGGACAATTTGAAGAGGCTACCCACCAGGcat > 1:14117970/1‑66 (MQ=2) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:9680845/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:5159550/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGt > 1:23511871/1‑68 (MQ=255) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCGGCTACCCACCAGGctt > 1:25766150/1‑66 (MQ=21) ccATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGAAGCCGCTACCCACCAGGcat > 1:6257018/1‑66 (MQ=21) ccATGGGTTACTGGCATCGTTTTATTTCGCATCCCCTGGGCACTTAGAAGAGCCTACCCACCAGGcat > 1:33102408/1‑66 (MQ=14) cATGGGTTAGTGGCTTAGTTTGCTTTCGCATCGCCTGGGCAATTTGTAGAGCCTACCCACCAGGCGtt < 1:30759659/68‑1 (MQ=21) cATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGtt < 1:32191488/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:20542815/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:36733763/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:14827793/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:7192959/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:8562002/68‑1 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:30530878/1‑68 (MQ=255) ggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTta > 1:28695939/1‑68 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa > 1:28394421/1‑68 (MQ=255) aCTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATaaa > 1:12038103/1‑68 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCTGTCTTATaaag > 1:26097332/1‑66 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:2642235/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:19762640/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTTATAAATg > 1:1515614/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:9254246/1‑67 (MQ=255) tgttttgCTTTGGCATCGCGTGGGCAATTTGAAGAGCCTACCCACCAGGCGTTCTTATAAATgcctcc < 1:34492224/65‑1 (MQ=18) tttttCTTTCGTATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24106414/67‑1 (MQ=255) tttttCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:8211466/67‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:34036057/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:18901218/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:15553525/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:18500200/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:27609131/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10803698/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:32836934/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:16984727/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17971133/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:29361226/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17588822/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24576269/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:23945544/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:4954022/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:6605606/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:6723627/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:884309/68‑1 (MQ=255) ttttCTTTCGGATCGCCTGGGCAATTTTCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:35984628/67‑1 (MQ=38) ttttCTTTCGCAGCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:21253265/67‑1 (MQ=255) cattctttgGCAACGCGTGGGCAATCTTTCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:13667630/65‑1 (MQ=17) ttctttcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:4000685/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:3712809/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:16554568/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:24977056/68‑1 (MQ=255) ttctttcGCAGCTCCTGTGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:1904292/68‑1 (MQ=39) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCCCAAGGCGTTCTTATAAATGCCGCCGTTATAg > 1:17373057/1‑68 (MQ=255) ttcGCATCGCGTGGGCAATTTGTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:23072720/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCGTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:34777298/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:22248797/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:32475803/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:16025596/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:19505306/1‑68 (MQ=255) ttcGCACCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:1469489/68‑1 (MQ=255) atcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:13933003/67‑1 (MQ=255) atcGCATCCCCTGGGCAATCTTACGCGCCTACCCACCATGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:14538157/67‑1 (MQ=255) cGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATaa < 1:20679592/68‑1 (MQ=255) gCATCGTCTGTGAAATTTGCAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:28070191/68‑1 (MQ=37) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACg < 1:12712230/67‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:33770725/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:4514854/68‑1 (MQ=255) gcgtGGGCAATTTTTCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:35476548/65‑1 (MQ=25) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:21683859/68‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:18358789/68‑1 (MQ=255) gtGGGCAATTTTTAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:2274995/67‑1 (MQ=37) cTGGGCAATTTTGCGCGCCAACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:21044972/68‑1 (MQ=38) cTGGGCAATTTTAAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:13482009/68‑1 (MQ=38) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:22118115/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:17467201/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:4378966/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:12351336/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCACGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:12968464/68‑1 (MQ=255) gCAATTTTTCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:31604221/67‑1 (MQ=38) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag > 1:26598581/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:33349767/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:10309639/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAAGGCGCCGTTATAGATGACGTACCAGGTag > 1:27827527/1‑68 (MQ=37) gCAATTTGACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:30226164/67‑1 (MQ=255) gCAATTTGACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:27197705/67‑1 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga > 1:5598740/1‑68 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTaga > 1:20212788/1‑68 (MQ=255) cAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTag < 1:13189381/67‑1 (MQ=255) aaTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTagaa > 1:21448802/1‑68 (MQ=255) | GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAAC > CP009273/3462374‑3462503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |