| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,439 | C→A | 6.4% | A258A (GCG→GCT) ‡ | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,439 | 0 | C | A | 6.4% | 1999.8 / 30.5 | 646 | A258A (GCG→GCT) ‡ | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base C (151/443); new base A (23/28); total (174/472) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 699 total aligned reads displayed.
GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTT > CP009273/3462374‑3462506 | ccgCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgc > 1:14798122/1‑67 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:461803/67‑1 (MQ=255) cgcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:5893081/67‑1 (MQ=255) cgcgcgAACAAAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCcgcg < 1:18511078/67‑1 (MQ=255) gcgcgAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGcc < 1:4235677/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:29650903/68‑1 (MQ=255) gAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAcc < 1:6380547/68‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTAccc < 1:20229431/67‑1 (MQ=255) aCATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACcca < 1:7582390/68‑1 (MQ=255) aTAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACccacc > 1:11447996/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGGCTACCCACCAGGc > 1:22857951/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:25518158/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGc > 1:11136091/1‑68 (MQ=255) cGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGACGCGCCTACCCACCAGGc > 1:23228213/1‑68 (MQ=255) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCAGAGGCTACCCACCAGGct > 1:30837841/1‑67 (MQ=21) gCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCGGGGCAATTTGAAGCGGCTACCCACCAGGca > 1:28166895/1‑67 (MQ=21) ccATGGGTTACTGGCATCGTTTTATTTCGCATCGCCAGGGCATTTAGAAGCGCCTACCCACCAGGcat > 1:12984012/1‑66 (MQ=17) cATGGGTTATTGGAATCGTCTGCTTTCGCATCGCCTGGGCAATCTGCCGCGCCTACCCACCAGGCGtt < 1:32918682/68‑1 (MQ=255) cATGGGTTACTGGAATCTTTTTCTTTCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGtt < 1:18918890/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:19601273/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:31728710/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:30564269/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:8562002/68‑1 (MQ=255) tGGGTTACTGGCATCGTTTTCTTTCCCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCt < 1:34645576/68‑1 (MQ=255) gggTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCtt > 1:26134891/1‑68 (MQ=255) gTTACTGGCATCGTTATCTATCGCATCGCCTGGGCAATATGCCGCGCCTACCCACCAGGCGTTCTta < 1:28567644/67‑1 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTGTtatataa > 1:19892890/1‑64 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAt > 1:32321996/1‑67 (MQ=255) tGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCAACAGGAGTTCTTATAAAt > 1:3638629/1‑67 (MQ=255) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCTTTTTTATATATg > 1:15981663/1‑67 (MQ=38) ggCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATg > 1:30392586/1‑67 (MQ=255) gCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgcc > 1:21059320/1‑68 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:36943676/68‑1 (MQ=255) tCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:14678495/68‑1 (MQ=255) tCGTTTGCTTTGGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATgccgcc < 1:13447169/68‑1 (MQ=255) gtgttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:24008649/65‑1 (MQ=255) gTTTTCTTTGGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:1210224/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:15553367/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:10803698/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:884309/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:13917485/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:6723627/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:35771728/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:31154280/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:4398441/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17588822/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:20543883/68‑1 (MQ=255) gTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:17971133/68‑1 (MQ=255) gTTTTCTATCGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:7208872/68‑1 (MQ=255) ttttATATCGCATCGCGTGGGCTATTTTCAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:9745187/67‑1 (MQ=25) tgttctctgGCACCGCGTGGGCAATCTTTAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:27053354/65‑1 (MQ=9) tcttctttcGTCTCCCCTGGGCAATTTTACGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:27685204/65‑1 (MQ=37) tcttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:21535127/65‑1 (MQ=255) tcttctttaGCATCTTCAGCGCAATTTGCCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGt < 1:13607419/65‑1 (MQ=25) ttctttgGCATCCCCTGGGCAATTTTTCGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:24237657/68‑1 (MQ=25) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:25894920/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:19134034/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:16554568/68‑1 (MQ=255) ttctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtat < 1:32690518/68‑1 (MQ=255) tctttgGCATCGCCTGGGCAATCTTAAGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtata < 1:5118310/68‑1 (MQ=21) tctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTtata < 1:14115714/68‑1 (MQ=255) ctttgGCATCCCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:5636406/68‑1 (MQ=38) ctttcGCATCGCCTGTGCTATATGCCGCGCCTACCCACCATGCGTTCTTTTAAATGCCGCCGTTATAg < 1:12362725/68‑1 (MQ=255) ctttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:4818725/68‑1 (MQ=255) ctatcGCATCGCCTGGGCAATTTGTCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAg < 1:2246153/65‑1 (MQ=255) ttcTCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:22133186/68‑1 (MQ=255) ttcGCCTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:23643808/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:21326948/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:17328446/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:1444792/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:35285155/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:34950574/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:20759895/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:2472893/68‑1 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt > 1:25095414/1‑68 (MQ=255) ttcGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:9454301/68‑1 (MQ=255) ttcGCATCGCCTGGGCAACTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:21845322/68‑1 (MQ=255) atcGCATCGCCTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:3776193/67‑1 (MQ=255) atcGCATCGCCTGGGCAATTTGACGAGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGAt < 1:33545163/67‑1 (MQ=38) gCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAAc < 1:29432775/68‑1 (MQ=255) aTCGCGTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGt < 1:26424129/68‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACg < 1:8211629/67‑1 (MQ=255) aTCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACg < 1:12712230/67‑1 (MQ=255) gCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTAcc < 1:18358789/68‑1 (MQ=255) gtGGGCAATTTGTAGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:29094463/67‑1 (MQ=38) cTGGGCAATTTTCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:26652164/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:15065451/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:17467201/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:34218310/68‑1 (MQ=255) cTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCAt < 1:8012807/68‑1 (MQ=255) tGGGCGATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATg > 1:9284529/1‑68 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTTTTATATACCGCGCCGTTATAGAGGACGTACCagg > 1:33920953/1‑63 (MQ=14) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:33166152/67‑1 (MQ=255) gCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTa < 1:10309639/67‑1 (MQ=255) aaTTTGCCGCGCCTACCCACCAGGCGTTCTTATAAAAGCCGCCGGTATAGATTACGGACCTGGTagaa > 1:13174781/1‑68 (MQ=37) ttGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATGACGTACCATGTagaaga > 1:27940233/1‑67 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:16967903/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCtt < 1:6627887/68‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt < 1:5311787/67‑1 (MQ=255) cgcgCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCt < 1:27361727/67‑1 (MQ=255) | GCCGCGCGAACATAACGCCATGGGTTACTGGCATCGTTTTCTTTCGCATCGCCTGGGCAATTTGCCGCGCCTACCCACCAGGCGTTCTTATAAATGCCGCCGTTATAGATAACGTACCATGTAGAAGAACCTT > CP009273/3462374‑3462506 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |