| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,462,676 | A→T | 5.8% | A179A (GCT→GCA) | chiA ← | periplasmic endochitinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,462,676 | 0 | A | T | 5.8% | 2089.2 / 21.9 | 617 | A179A (GCT→GCA) | chiA | periplasmic endochitinase |
| Reads supporting (aligned to +/- strand): ref base A (314/255); new base T (2/46); total (316/301) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.37e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 622 total aligned reads displayed.
CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGA > CP009273/3462609‑3462741 | ccGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTa > 1:32242864/1‑68 (MQ=255) ggTGACGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGc < 1:33664688/68‑1 (MQ=255) ggTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGc > 1:16907937/1‑68 (MQ=255) gAAGGCTGGGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCgg < 1:20441627/67‑1 (MQ=255) ggCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACg > 1:9588907/1‑68 (MQ=255) gCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa > 1:34882488/1‑68 (MQ=255) cTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGa < 1:20729002/67‑1 (MQ=255) cTGTGGTGCGGAAGTCCAGGACTGTGGATTACTGGCGTCTGTGATTTCCGTTGCCGTAGCGGCACGa < 1:20118231/67‑1 (MQ=255) gtggtgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACa < 1:26055930/68‑1 (MQ=255) gtggtgCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACa < 1:35210696/68‑1 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAAc > 1:19378873/1‑67 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACg < 1:22016797/68‑1 (MQ=255) gCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACg < 1:33884315/68‑1 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGc < 1:10993769/67‑1 (MQ=255) tGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGc < 1:28351341/67‑1 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:28701946/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:16391833/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:23146235/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:17828073/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:6593221/1‑68 (MQ=255) aGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATg > 1:18439679/1‑68 (MQ=255) tACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg > 1:8136021/1‑68 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATggg < 1:2165537/67‑1 (MQ=255) aCAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATgg < 1:32821355/66‑1 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCAGGAACATAACGCCATGGGtt > 1:7268366/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:22338490/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:2578084/1‑67 (MQ=255) aGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:36613926/1‑67 (MQ=255) ggACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGtt > 1:15472590/1‑66 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTggtt > 1:23373106/1‑65 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:24203085/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:9258032/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:24457142/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:36349025/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:35742178/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:32089824/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:26977952/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:34672724/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:27799154/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:30761607/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:4661744/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:13528620/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:7445082/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:14905838/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:15897940/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:16834959/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:12562219/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:6975673/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:18097797/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:36870936/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:4405942/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:12402663/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:9068168/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:11538781/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:20988595/1‑68 (MQ=255) aCTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTg > 1:8370400/1‑68 (MQ=255) tgtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGc > 1:32601643/1‑68 (MQ=255) tgtgGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGc < 1:33325541/68‑1 (MQ=255) ggATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatc > 1:35468504/1‑68 (MQ=255) gATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGcatca > 1:31629618/1‑68 (MQ=255) ttACTGTTCTGTGAGATTTCGGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt < 1:33209481/68‑1 (MQ=21) aCTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCAtt > 1:4216544/1‑66 (MQ=255) aCTGGTCTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGc < 1:19713733/68‑1 (MQ=255) ggTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCttt > 1:27223550/1‑68 (MQ=255) tttCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTc > 1:11433544/1‑68 (MQ=255) ttCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCg < 1:18972446/68‑1 (MQ=255) cTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCa > 1:23738246/1‑68 (MQ=255) gagaTTTCCGTTGCC‑TAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:2920804/68‑1 (MQ=255) tgaTTTCCGTTGCCGTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:6415571/67‑1 (MQ=255) gaTTTCCGTTGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:19135021/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa > 1:14052487/1‑67 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:575398/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:5463826/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa < 1:25465403/67‑1 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCa > 1:8834027/1‑67 (MQ=255) gaTTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTATTCGCATCa > 1:3945619/1‑67 (MQ=255) tttCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCAcc < 1:4997524/67‑1 (MQ=255) cGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGAc < 1:14381801/68‑1 (MQ=255) cGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGAc < 1:34207513/68‑1 (MQ=255) cGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGAc < 1:2886125/68‑1 (MQ=255) gTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACa < 1:12880210/68‑1 (MQ=255) tGCGGTAGCGGCACGAACTTAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:21396333/67‑1 (MQ=255) tGCCGTTGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:26573444/67‑1 (MQ=255) tGCCGTTGCCGCGCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:25072356/67‑1 (MQ=18) tGCCGTTGCCGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:3106097/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:10303172/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:1754980/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:6152216/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:22774120/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:24845593/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:36039328/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAg < 1:30255775/67‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:1963819/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:17226456/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:7750258/68‑1 (MQ=255) tGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGt < 1:10076423/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:12027427/68‑1 (MQ=255) gCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGtt < 1:2990717/68‑1 (MQ=255) gTAGCGGCTCGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTaga < 1:26435347/68‑1 (MQ=255) gTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTaga < 1:5206271/68‑1 (MQ=255) | CCGGTGAAGGCTGTGGTGCTGAAGTACAGGACTGTGGATTACTGGTTTCTGAGATTTCCGTTGCCGTAGCGGCACGAACATAACGCCATGGGTTGCTGGCATCATTGCTTTTCGCATCACCCGGACAGTTAGA > CP009273/3462609‑3462741 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |