| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 298,191 | T→G | 7.7% | S32R (AGC→CGC) | paoA ← | PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 298,191 | 0 | T | G | 7.7% | 1311.2 / 12.4 | 434 | S32R (AGC→CGC) | paoA | PaoABC aldehyde oxidoreductase, 2Fe‑2S subunit |
| Reads supporting (aligned to +/- strand): ref base T (71/323); new base G (37/2); total (109/325) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-22 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 458 total aligned reads displayed.
CTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATCT > CP009273/298125‑298258 | tGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCa < 1:6861164/68‑1 (MQ=255) tGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCa < 1:31332969/68‑1 (MQ=255) tGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCa < 1:15774232/68‑1 (MQ=255) gCCGGTACGCTTGTCTCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:32732577/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:1732762/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:34544392/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:22892325/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:33134370/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:34991013/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:35243713/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:36901014/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:31536137/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:4389538/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:4557381/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:4820831/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:6035772/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:11110486/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:27623219/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:1460633/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:10084347/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:29933813/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:8899144/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:7140282/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:30229191/68‑1 (MQ=255) gCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCAc < 1:13889193/68‑1 (MQ=255) cGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACt < 1:24709661/67‑1 (MQ=255) aaCGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTTCGGCGCTCCCtttta > 1:29453729/1‑66 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:30768787/68‑1 (MQ=255) ttGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGa < 1:17845499/68‑1 (MQ=255) tGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCCGCCCGGGTGGGGGGCGCCCTTTAATcc > 1:21378262/1‑64 (MQ=255) cgccAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATcgc < 1:21618585/68‑1 (MQ=255) ccAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCGCACTTTTATAACATagcg > 1:5339109/1‑67 (MQ=255) ccAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCGGTTGCGGCGCTCACGTTAATCAGATcgcg > 1:20226531/1‑67 (MQ=255) ccAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCGGTTGCGGCGCGCACATTAATGAGATggcg > 1:23402346/1‑67 (MQ=255) ccAGCGTAGAATGAGGATAAACCACGGCGGTCGCCCCTGGTTCGGGGCGCACGTTAATCAGATagcg > 1:1502582/1‑67 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACggg < 1:25569835/67‑1 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACggg < 1:26927881/67‑1 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACggg < 1:17022565/67‑1 (MQ=255) gTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGt < 1:1603451/68‑1 (MQ=255) aTGAGGATAAACCTCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:10820656/67‑1 (MQ=255) aTGAGGATAAACCAGGGCGGTCGGCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:9044458/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaaa > 1:7507198/1‑67 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:3864502/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:33499710/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:34281849/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:23633671/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:2329346/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:22590551/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:21036329/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:18864273/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:16685650/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:1548401/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:14902451/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:680518/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:11930519/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCaaa < 1:10510846/67‑1 (MQ=255) aTGAGGATAAACCACGGCGGTCGCCGCGGTTGGGGCGCGCACATTTATAAGATCGCGACGGGGCAAAc > 1:3712905/1‑68 (MQ=255) tGAGGATAACCCACGGCGGGCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACt < 1:9630656/68‑1 (MQ=255) tGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACt < 1:12517118/68‑1 (MQ=255) tGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACt < 1:23830487/68‑1 (MQ=255) tGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACt < 1:25348225/68‑1 (MQ=255) gATAAACCACGGCGGTCGCCCCTGTTGCGGCGCGCACTTTTATAAAATAGCGACGGGTGAAAATTaaa > 1:3246222/1‑68 (MQ=255) aTAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTaa < 1:24414025/66‑1 (MQ=255) aTAAACCACGGCGGTCGCCGCTGGTGCGGGGCGCACTTTTATAAGATCGCGCCGGGGGAAAATTaaaa > 1:21985879/1‑67 (MQ=255) ccACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGc > 1:7017361/1‑68 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:18615858/68‑1 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:35723214/68‑1 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:26731941/68‑1 (MQ=255) aCGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCgg < 1:28159064/68‑1 (MQ=255) ggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGt < 1:18233757/68‑1 (MQ=255) ggTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGt < 1:7305240/68‑1 (MQ=255) gccgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCtt < 1:8655310/68‑1 (MQ=255) gccgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCtt < 1:20746658/68‑1 (MQ=255) gccgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCtt < 1:7934730/68‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:19160563/67‑1 (MQ=255) cgcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTc < 1:27252038/67‑1 (MQ=255) gcTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTccc > 1:29200982/1‑68 (MQ=255) gTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCaa > 1:13514864/1‑67 (MQ=255) gTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAc < 1:36526033/68‑1 (MQ=255) gTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAc < 1:13675272/68‑1 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAccc < 1:6402718/68‑1 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAccc > 1:11652061/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAccc > 1:11462771/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAAcc < 1:8434496/67‑1 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTACCAAccc > 1:24838680/1‑68 (MQ=255) tGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCCGCTCGTGCTTCCCAAccc > 1:6269488/1‑68 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt > 1:12337290/1‑68 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt < 1:3998286/68‑1 (MQ=255) ggcgCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGAt < 1:1973009/68‑1 (MQ=255) cgcTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTa > 1:16436397/1‑68 (MQ=255) cgcTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTa > 1:27449516/1‑68 (MQ=255) gcTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTAt < 1:31850402/68‑1 (MQ=255) tCAGTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:20580198/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:14156495/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:4991284/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:19486567/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:20032307/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:33955781/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATc < 1:25976495/67‑1 (MQ=255) tCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATCt < 1:28610004/68‑1 (MQ=255) | CTGCCGGAACGCTTGCCGCCAGCGTAGAATGAGGATAAACCACGGCGGTCGCCGCTGTTGCGGCGCTCACTTTAATCAGATCGCGACGGGTCAAACTTAAATCGTGCGGCTCGTGCTTCCCAACCCGATTATCT > CP009273/298125‑298258 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |