| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 4,264,193 | C→G | 6.5% | G47G (GGC→GGG) | ssb → | single‑stranded DNA‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 4,264,193 | 0 | C | G | 6.5% | 2064.3 / 84.6 | 588 | G47G (GGC→GGG) | ssb | single‑stranded DNA‑binding protein |
| Reads supporting (aligned to +/- strand): ref base C (240/308); new base G (27/12); total (267/321) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 630 total aligned reads displayed.
AAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC > CP009273/4264127‑4264259 | aaaTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCg > 1:1934547/1‑68 (MQ=255) aTGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgag < 1:7008798/68‑1 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:13576838/1‑68 (MQ=255) tggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCgaga > 1:26080257/1‑68 (MQ=255) ggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGAt > 1:15727605/1‑68 (MQ=255) ggtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGAt > 1:22711231/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:25366693/1‑68 (MQ=255) gtggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg > 1:31770094/1‑68 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:18299174/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:37084681/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:10785982/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:31241881/67‑1 (MQ=255) tggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATg < 1:21354442/67‑1 (MQ=255) ggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa < 1:25595027/68‑1 (MQ=255) ggCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGaa > 1:9265993/1‑68 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAg < 1:16781108/67‑1 (MQ=255) gcAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGa < 1:3653513/68‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAActg < 1:36340122/67‑3 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAg < 1:32474696/67‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGa < 1:29588343/68‑1 (MQ=255) tGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGa < 1:33251841/68‑1 (MQ=255) cAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACt < 1:14734194/67‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:35603362/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:23463372/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:25214471/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:26341505/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:30519216/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:11508117/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:15278901/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:9426570/68‑1 (MQ=255) aaCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGa < 1:16507469/68‑1 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:5750486/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:12213227/1‑68 (MQ=255) aCATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGaa > 1:22383902/1‑68 (MQ=255) aTTACGCTGGCTACTTCCGAATCCTGGCGTGGTAAAGCGACCGGCGAGATGAAAGAACAGACTGAATg > 1:34281471/1‑68 (MQ=255) aTTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATg > 1:5340296/1‑68 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCTACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:28034248/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:24723293/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:14003121/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:7675679/68‑1 (MQ=255) tACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGc < 1:6681060/68‑1 (MQ=255) cGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAc < 1:1433625/68‑1 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGCCCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:27512973/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:29710023/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:28554266/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:1046036/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:8234755/1‑68 (MQ=255) gCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:8984102/1‑68 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:32998456/1‑67 (MQ=255) cTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCAcc > 1:28388481/1‑67 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:28813218/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:1865464/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:26725921/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:32899657/68‑1 (MQ=255) tGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:34001122/68‑1 (MQ=255) tGGCTACTTCCGAATCCT‑GCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACcgc < 1:17126992/67‑1 (MQ=255) cTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGtt > 1:7278486/1‑68 (MQ=255) cTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGt < 1:13290763/67‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:8094970/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:6503395/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:26915659/68‑1 (MQ=255) aCTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt < 1:10230131/68‑1 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:29989562/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:15508981/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:21032103/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTtgt > 1:32059117/1‑65 (MQ=255) tCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCt < 1:11401322/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:31467502/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:18074909/68‑1 (MQ=255) cGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGt < 1:5020670/68‑1 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:23967926/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:18984329/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:19734732/1‑68 (MQ=255) aTCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCg > 1:20435600/1‑68 (MQ=255) gcTGGCGTGACAAACAGACGGGGGAGATGCGGCAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGc > 1:34615631/2‑68 (MQ=255) ggCGTGATAAATCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCaa > 1:22907186/1‑67 (MQ=255) cGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCaa > 1:35002699/1‑65 (MQ=255) cGTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCaagc < 1:22100162/67‑3 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg > 1:24410449/1‑68 (MQ=255) gTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTg < 1:21742812/68‑1 (MQ=255) gTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCtc < 1:30312965/68‑2 (MQ=255) gTGACAAACAGACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCt < 1:4384841/67‑1 (MQ=255) gATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTgg < 1:19978430/67‑1 (MQ=255) tAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCa < 1:16070140/67‑1 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg > 1:32704909/1‑68 (MQ=255) aGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAg > 1:4770634/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:13052103/1‑68 (MQ=255) gCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGt > 1:1113593/1‑68 (MQ=255) cGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTg > 1:3998161/1‑68 (MQ=255) agACGGGGGAGATGCGGGAGCAGAAAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCACGCCGAAGTg > 1:6080121/2‑68 (MQ=255) gACGGGGGAGATGCGGGAGCAGACAGAATGGCATCGCGTGGTGCTGTTCGGCAAGCTCGCGGAAGTg > 1:17311417/1‑67 (MQ=255) gACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTgg < 1:28968911/68‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:35949505/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:33517494/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:20734894/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:17701029/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:3099029/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:12523483/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:10000779/67‑1 (MQ=255) cGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGgcgagc < 1:27827135/67‑1 (MQ=255) | AAATGGTGGCGCAGTTGCCAACATTACGCTGGCTACTTCCGAATCCTGGCGTGATAAAGCGACCGGCGAGATGAAAGAACAGACTGAATGGCACCGCGTTGTGCTGTTCGGCAAACTGGCAGAAGTGGCGAGC > CP009273/4264127‑4264259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |