| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,177,255 | 0 | G | T | 36.2% | 68.8 / 20.6 | 58 | intergenic (‑51/+257) | gatY/fbaB | tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY/fructose‑bisphosphate aldolase class I |
| Reads supporting (aligned to +/- strand): ref base G (10/27); new base T (15/6); total (25/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.03e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA > NC_000913/2177106‑2177393 | cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg < 2:232333/150‑1 (MQ=255) acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAATTATTTGt > 2:2098532/1‑150 (MQ=255) acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGt > 2:2098460/1‑150 (MQ=255) cGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAat < 2:542842/150‑1 (MQ=255) tttCGAGATTTTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACTTACATTTTCATATCCTGTCGGTTGTTTTAGTTTTCAAAAAATAAAGAAATTATTTTTTTTTAAatat > 1:1531992/1‑150 (MQ=255) tCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCg < 1:153888/150‑1 (MQ=255) gagaTTGTGATTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCgag > 2:1749259/1‑150 (MQ=255) aaTATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGAGTTGTTCAGCATCTGCTTTGTCGATACCACGGACATTTTCATCTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATc < 1:1957346/150‑1 (MQ=255) aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaaaa > 1:2132318/1‑150 (MQ=255) aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa < 1:475237/150‑1 (MQ=255) aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCACaaaaa > 1:2132513/1‑150 (MQ=255) gCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGa < 1:2011482/150‑1 (MQ=255) ccGTAACCGAATAACCGCGGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTAATATCCTTTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGAc < 1:1647233/150‑1 (MQ=255) cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTCAAAATATAATTTAAATATTTTTTTTTAAATATGGAGATTAAGATGACAAAAACGACa > 1:324803/1‑150 (MQ=255) ggAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTAATTTTTATTATCCTGTGTTTTGTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACaa < 2:1902607/150‑1 (MQ=255) gAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCTATACCACGTAAATTTTCATATCCTTTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAat < 2:1877861/150‑1 (MQ=255) ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAatatt > 2:940578/1‑149 (MQ=255) gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTTTTTTTAAATATAGAGATAAAGATAACAAAAAAGACAATATTaa > 2:1355131/1‑150 (MQ=255) ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAAAATAATTAAATTAATTTTTTTTAAATATAGAGATAACGATAAAAAAAAAGAAAAAAATAAAaatat > 1:1843810/1‑150 (MQ=255) ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTCAAAATATAATGAAAATATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAAAATGAAAaatat > 1:1843904/1‑150 (MQ=255) ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATAACAAAAACGACAATATGAAAattat > 2:202767/1‑150 (MQ=255) cgccgcGCTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGAACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTAAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattatt < 1:429157/150‑1 (MQ=255) cgcgCTGTGCGTTGTTCAGCATATGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTACGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAAAAACACGACAATATGAAAATTATTCga < 2:931866/150‑1 (MQ=255) gcTGTGCGTTGTTCAGGATCTGGTTTGTCTATACCACGTACATTTTAATATCCTGTCGTTTTTTTTCGATTACAAAATATAATTAAATTATTTGTTTTTAAATTTCGTGATAACGATAACAAAAACGACAATATGAAAATTATTCgagga < 1:1705175/150‑1 (MQ=255) gcTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATAGCCTGTCGTTTGTTTTCGATTTCAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgagga < 1:627912/150‑1 (MQ=255) tgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCAAAAAAACGACAATATGAAAATTATTAGAGGAGTGa > 2:1340835/1‑150 (MQ=255) cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATTAAAATTATTAGATGAGTGaaa > 1:2142573/1‑150 (MQ=255) ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGTAAATATTTTTTTTTAAATATAGAGATAACGAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGTAATGCaa > 1:467003/1‑150 (MQ=255) tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACAAAATGAAAATTATTAGAGGAGTGAAATGCaaa > 2:678610/1‑150 (MQ=255) tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg < 2:981906/150‑1 (MQ=255) gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATGAAATTAATTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAAATATTCGAGGAGTGAAAGGCAAAAAAACGGcc > 1:1747724/1‑150 (MQ=255) tCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTAATTTTTTTTTAATATCGAGATAACGATAACAAAAACGACAATATTAAAATTATTAGAGGAGTGTAAGGCAAAAAAAAGGCCTCCCGa > 2:397398/1‑150 (MQ=255) cGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTTTTTCAAAATATAATTAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGAt < 1:1749259/150‑1 (MQ=255) ccACGTAAATTTTCATATCCTGTCGTTTTTTTTCGATTACAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGa < 2:14203/150‑1 (MQ=255) cACGTACATTTTCATATCCTGTCGTTTGTTTTGGGTTTCAAAATATAATGAAATTATTTTTTTTTTAATATAGTGATTACGATCACAAAAAAGACAATATGTAAATTATTCGGGGTGTGTAAGGCAAAAAAACGGCCTCCCGATAGGGaa > 2:1047045/1‑150 (MQ=255) cACGTAAATTTTCATATCCTGTCGTTTGTTTTCGATTACAAAATATATTCAAATTATTTGTTTTTAAATATCGATATAACGATAACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa < 2:548108/150‑1 (MQ=255) aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg < 2:1381954/150‑1 (MQ=255) taaaTTTTCATATCCTGTCTTTTGTTTTCTTTTTAAAAATATAATTAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg < 1:202767/147‑1 (MQ=255) aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATATTGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt < 2:1052312/150‑1 (MQ=255) aCATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGt < 2:1052303/150‑1 (MQ=255) cATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa < 2:1957695/150‑1 (MQ=255) ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGTTCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc > 2:1835739/1‑150 (MQ=255) gtttgtttTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt < 1:324917/150‑1 (MQ=255) tgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa > 2:598203/1‑150 (MQ=255) ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGTGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg > 2:478036/1‑150 (MQ=255) tttCGATTTCAAAATATACTGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc < 2:917235/150‑1 (MQ=255) ttCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc < 2:2129656/150‑1 (MQ=255) gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa > 1:1626260/1‑150 (MQ=255) aTTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt < 1:457053/150‑1 (MQ=255) ttCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGc > 1:203451/1‑150 (MQ=255) aaaaTATAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt < 1:1916109/150‑1 (MQ=255) aaaaTATAATGAAATTATTTGTTTTTAAATATCGATATAACGATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt < 1:729493/150‑1 (MQ=255) aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt < 1:729464/150‑1 (MQ=255) atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa < 1:1971927/150‑1 (MQ=255) taaaaTCAAATTATTTGTTTATAAATATCGATATAACTATCAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaa < 1:625202/147‑1 (MQ=255) ataATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa < 2:1843904/150‑1 (MQ=255) ataATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa < 2:1843810/150‑1 (MQ=255) ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa < 1:1044113/150‑1 (MQ=255) tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa > 2:938949/1‑150 (MQ=255) | CACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA > NC_000913/2177106‑2177393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |