Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,214,649 A→T I124F (ATC→TTC)  metA → homoserine O‑succinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,214,6490AT99.1% 396.7 / NA 107I124F (ATC→TTC) metAhomoserine O‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (55/51);  total (56/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATA  >  NC_000913/4214502‑4214793
                                                                                                                                                   |                                                                                                                                                
cGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACCTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTc                                                                                                                                                <  1:1984342/150‑1 (MQ=255)
cGTGAATCGCGCAACACGCCCGCAGAGAATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGCACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTc                                                                                                                                                <  2:205951/150‑1 (MQ=255)
      tCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAg                                                                                                                                          <  2:1970572/150‑1 (MQ=255)
        gcgcAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGt                                                                                                                                        >  2:1769809/1‑150 (MQ=255)
        gcgcAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGt                                                                                                                                        >  2:1323117/1‑150 (MQ=255)
           cAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCTAACAGGTGCt                                                                                                                                     <  1:920912/150‑1 (MQ=255)
            aaCACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTg                                                                                                                                    <  2:1992877/150‑1 (MQ=255)
            aaCACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTg                                                                                                                                    <  1:750076/150‑1 (MQ=255)
             acacGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCTTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTgg                                                                                                                                   <  2:662021/150‑1 (MQ=255)
               acGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAg                                                                                                                                 <  2:1843977/150‑1 (MQ=255)
               acGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAg                                                                                                                                 >  1:1558282/1‑150 (MQ=255)
               acGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAg                                                                                                                                 >  1:1557311/1‑150 (MQ=255)
                   ccGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGt                                                                                                                             >  2:198682/1‑150 (MQ=255)
                        gagCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaaa                                                                                                                        <  1:377718/150‑1 (MQ=255)
                        gagCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGaaa                                                                                                                        <  1:377838/150‑1 (MQ=255)
                             tCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGAtaa                                                                                                                   >  2:1215855/1‑148 (MQ=255)
                             tCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCa                                                                                                                   >  1:1175994/1‑150 (MQ=255)
                             tCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCa                                                                                                                   <  1:367234/150‑1 (MQ=255)
                               tGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACg                                                                                                                 >  1:831880/1‑150 (MQ=255)
                                   caacTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCAc                                                                                                             >  1:1804525/1‑150 (MQ=255)
                                     acTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCt                                                                                                           <  1:928239/150‑1 (MQ=255)
                                        tCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGa                                                                                                        <  1:1245465/150‑1 (MQ=255)
                                        tCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGa                                                                                                        <  2:940985/150‑1 (MQ=255)
                                           aCTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGc                                                                                                     >  2:1676413/1‑150 (MQ=255)
                                             tGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTg                                                                                                   <  1:498293/150‑1 (MQ=255)
                                              gTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGt                                                                                                  <  1:284671/150‑1 (MQ=255)
                                               tAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGtt                                                                                                 >  1:85804/1‑150 (MQ=255)
                                                 aCTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCTACGCTGTTTg                                                                                               >  1:1152332/1‑150 (MQ=255)
                                                   tttGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTc                                                                                             >  2:909164/1‑150 (MQ=255)
                                                    ttGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTct                                                                                            <  2:510463/150‑1 (MQ=255)
                                                     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                                                                           >  1:1589222/1‑150 (MQ=255)
                                                     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                                                                           >  1:1459066/1‑150 (MQ=255)
                                                     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                                                                           >  1:1930356/1‑150 (MQ=255)
                                                     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                                                                           <  1:1211131/150‑1 (MQ=255)
                                                     tGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTctg                                                                                           <  1:1211209/150‑1 (MQ=255)
                                                         gATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTgg                                                                                       >  1:493230/1‑150 (MQ=255)
                                                          atatTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTggg                                                                                      >  2:702917/1‑150 (MQ=255)
                                                           tatTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGc                                                                                     <  2:1547569/150‑1 (MQ=255)
                                                            atTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACTTCACCTCGACGCTGTTTGTCTGCTGGGCg                                                                                    >  1:386792/1‑150 (MQ=255)
                                                              tCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGt                                                                                  >  1:941977/1‑150 (MQ=255)
                                                               cAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTa                                                                                 <  1:897677/150‑1 (MQ=255)
                                                                aGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTAc                                                                                >  1:1133222/1‑150 (MQ=255)
                                                                  gATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAg                                                                              <  2:599601/150‑1 (MQ=255)
                                                                     cAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGcc                                                                           <  2:1101651/150‑1 (MQ=255)
                                                                      aGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcg                                                                          >  2:1164598/1‑150 (MQ=255)
                                                                       gAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACATGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgc                                                                         >  2:1514546/1‑150 (MQ=255)
                                                                       gAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCcgc                                                                         >  1:714143/1‑150 (MQ=255)
                                                                         aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGATAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                                                                       <  1:1986206/150‑1 (MQ=255)
                                                                         aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                                                                       <  1:1985967/150‑1 (MQ=255)
                                                                         aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                                                                       <  2:51524/150‑1 (MQ=255)
                                                                         aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                                                                       >  1:729264/1‑150 (MQ=255)
                                                                         aCTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCgcgc                                                                       <  2:51502/150‑1 (MQ=255)
                                                                          cTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCt                                                                      >  1:509548/1‑150 (MQ=255)
                                                                           tttGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTc                                                                     >  1:2071653/1‑150 (MQ=255)
                                                                               aCGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAata                                                                 >  2:215596/1‑150 (MQ=255)
                                                                                 ggTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATc                                                               <  1:465505/150‑1 (MQ=255)
                                                                                 ggTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATc                                                               <  2:145675/150‑1 (MQ=255)
                                                                                 ggTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATc                                                               <  2:145601/150‑1 (MQ=255)
                                                                                  gTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATcc                                                              <  2:736965/150‑1 (MQ=255)
                                                                                  gTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATcc                                                              >  2:1401702/1‑150 (MQ=255)
                                                                                    ttGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGATGTTTGTCTGCTGGGCGGTACAGGCCGCGCACAATATActc                                                            >  1:788707/1‑150 (MQ=255)
                                                                                      gATTGTAACTGGTGCGCCGCTGGGCCTGGTGGTGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTAGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCctctt                                                          <  1:1552304/150‑2 (MQ=255)
                                                                                      gATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTa                                                          >  1:1437215/1‑150 (MQ=255)
                                                                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACg                                                        <  2:1509570/150‑1 (MQ=255)
                                                                                        ttGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACg                                                        >  1:1340941/1‑150 (MQ=255)
                                                                                          gTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGc                                                      >  2:1605839/1‑150 (MQ=255)
                                                                                           tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCa                                                     >  2:581630/1‑150 (MQ=255)
                                                                                           tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCa                                                     >  2:1921197/1‑150 (MQ=255)
                                                                                           tAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCa                                                     >  2:1747484/1‑150 (MQ=255)
                                                                                              cTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTc                                                  <  1:1545777/150‑1 (MQ=255)
                                                                                              cTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTc                                                  >  1:116037/1‑150 (MQ=255)
                                                                                               tGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTcc                                                 <  1:1269412/150‑1 (MQ=255)
                                                                                                 gTGCGCCGCGGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAATCAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTa                                               >  1:1023754/1‑150 (MQ=255)
                                                                                                  tGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGATGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTaa                                              <  1:1634006/150‑1 (MQ=255)
                                                                                                     gccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCa                                           >  1:1367169/1‑150 (MQ=255)
                                                                                                     gccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCa                                           <  1:76334/150‑1 (MQ=255)
                                                                                                     gccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCa                                           >  1:269026/1‑150 (MQ=255)
                                                                                                     gccgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCa                                           <  1:1817557/150‑1 (MQ=255)
                                                                                                       cgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCaaa                                         >  2:1756487/1‑150 (MQ=255)
                                                                                                       cgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCaaa                                         >  2:1756476/1‑150 (MQ=255)
                                                                                                       cgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCaaa                                         >  1:914004/1‑150 (MQ=255)
                                                                                                       cgcTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCaaa                                         >  2:1028745/1‑150 (MQ=255)
                                                                                                            ggCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGc                                    >  2:1392094/1‑150 (MQ=255)
                                                                                                              ccTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCAc                                  <  1:1251135/150‑1 (MQ=255)
                                                                                                                    ggAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGaaaa                            >  1:1604727/1‑150 (MQ=255)
                                                                                                                     gAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGaaaaa                           <  1:702917/150‑1 (MQ=255)
                                                                                                                     gAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGaaaaa                           <  2:1208419/150‑1 (MQ=255)
                                                                                                                     gAGTTTAATGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGaaaaa                           >  1:490267/1‑150 (MQ=255)
                                                                                                                           aaTGATGTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAActctct                     >  1:1706536/1‑150 (MQ=255)
                                                                                                                            atgatgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTg                    <  2:493230/150‑1 (MQ=255)
                                                                                                                                tgTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGt                <  1:1453774/150‑1 (MQ=255)
                                                                                                                                 gTCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGtt               >  2:615383/1‑150 (MQ=255)
                                                                                                                                  tCGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGttt              >  2:752738/1‑150 (MQ=255)
                                                                                                                                   cGCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTa             <  2:1437215/150‑1 (MQ=255)
                                                                                                                                    gCTTACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTAc            >  1:2060505/1‑150 (MQ=255)
                                                                                                                                      ttACTGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGa          >  2:1859185/1‑150 (MQ=255)
                                                                                                                                          tGGCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGcat      >  2:2125054/1‑150 (MQ=255)
                                                                                                                                           ggCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGcatc     <  2:388309/150‑1 (MQ=255)
                                                                                                                                            gCCGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGcatca    >  2:617098/1‑150 (MQ=255)
                                                                                                                                              cGCAGTTCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCata  >  1:234380/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATA  >  NC_000913/4214502‑4214793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: