BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,998	C→T	G124S (GGT→AGT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,998	0	C	T	99.1%	415.5 / NA	113	G124S (GGT→AGT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (47/65); minor base A (1/0); total (48/65)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

GCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGC  >  NC_000913/1143852‑1144144

gCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGc                                                                                                                                                 >  1:633815/1‑150 (MQ=255)

  cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGTCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCa                                                                                                                                               >  1:899588/1‑150 (MQ=255)

  cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCa                                                                                                                                               <  2:548819/150‑1 (MQ=255)

  cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCa                                                                                                                                               >  2:2017844/1‑150 (MQ=255)

  cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACACGCCa                                                                                                                                               >  2:1492689/1‑150 (MQ=255)

  cAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTGTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCa                                                                                                                                               >  1:131726/1‑150 (MQ=255)

    gCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGa                                                                                                                                             <  1:1752016/150‑1 (MQ=255)

    gCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGa                                                                                                                                             <  1:1751933/150‑1 (MQ=255)

     cGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGAc                                                                                                                                            <  2:473607/150‑1 (MQ=255)

      ggTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACt                                                                                                                                           <  2:1551840/150‑1 (MQ=255)

             aCGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAg                                                                                                                                    >  1:1055035/1‑150 (MQ=255)

                     ccccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTaa                                                                                                                            <  1:1489743/150‑1 (MQ=255)

                     ccccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTaa                                                                                                                            <  1:1489732/150‑1 (MQ=255)

                       ccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATg                                                                                                                          <  2:2003142/150‑1 (MQ=255)

                       ccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATg                                                                                                                          <  2:1866421/150‑1 (MQ=255)

                          tGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCcg                                                                                                                       >  1:2095106/1‑150 (MQ=255)

                           gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCcgc                                                                                                                      <  1:200384/150‑1 (MQ=255)

                                ccGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCtt                                                                                                                 <  1:1351404/150‑1 (MQ=255)

                                 cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCttt                                                                                                                <  1:866440/150‑1 (MQ=255)

                                  ggCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttg                                                                                                               >  1:707219/1‑150 (MQ=255)

                                   gCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCtttat                                                                                                              <  2:1262556/150‑3 (MQ=255)

                                     aGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgttg                                                                                                            >  2:308138/1‑150 (MQ=255)

                                            ggCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGct                                                                                                     >  2:862345/1‑150 (MQ=255)

                                             gCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctc                                                                                                    <  2:512905/150‑1 (MQ=255)

                                              cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTTCCGCGctct                                                                                                   >  1:611440/1‑150 (MQ=255)

                                              cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctct                                                                                                   >  1:611517/1‑150 (MQ=255)

                                               ttGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                                                                                  >  2:1495330/1‑150 (MQ=255)

                                                tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                                                                                 >  2:33707/1‑150 (MQ=255)

                                                tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                                                                                 <  1:534261/150‑1 (MQ=255)

                                                 gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                                                                                >  1:891264/1‑150 (MQ=255)

                                                 gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                                                                                >  2:1704836/1‑150 (MQ=255)

                                                 gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttct                                                                                                <  1:450205/150‑1 (MQ=255)

                                                  ccAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTcttctt                                                                                               >  1:126691/1‑150 (MQ=255)

                                                    aGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTa                                                                                             <  2:786870/150‑1 (MQ=255)

                                                     gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                                                                            <  2:1582816/150‑1 (MQ=255)

                                                     gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                                                                            <  1:1730063/150‑1 (MQ=255)

                                                     gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                                                                            <  2:432550/150‑1 (MQ=255)

                                                     gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTAt                                                                                            >  1:336372/1‑150 (MQ=255)

                                                        cttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGa                                                                                         >  2:621949/1‑150 (MQ=255)

                                                         ttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGAt                                                                                        <  2:2009596/150‑1 (MQ=255)

                                                         ttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGAt                                                                                        >  2:2027643/1‑150 (MQ=255)

                                                         gtcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGAt                                                                                        <  2:2009657/149‑1 (MQ=255)

                                                          tcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                                                                       <  2:424641/150‑1 (MQ=255)

                                                           cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCt                                                                                      <  1:1495330/150‑1 (MQ=255)

                                                           cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCt                                                                                      >  2:321091/1‑150 (MQ=255)

                                                             tttAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGa                                                                                    <  1:347356/150‑1 (MQ=255)

                                                              ttAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGaa                                                                                   >  2:1656863/1‑150 (MQ=255)

                                                                 aTTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                                                                                <  2:1675476/150‑1 (MQ=255)

                                                                          ggTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                                                                       >  2:1637840/1‑150 (MQ=255)

                                                                          ggTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                                                                       <  1:880517/150‑1 (MQ=255)

                                                                           gtcgtcGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTg                                                                      >  2:513280/1‑150 (MQ=255)

                                                                             cgtcgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGAc                                                                    <  2:1193910/150‑1 (MQ=255)

                                                                             cgtcgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGAc                                                                    <  2:102463/150‑1 (MQ=255)

                                                                               tcgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCt                                                                  >  2:73442/1‑150 (MQ=255)

                                                                               tcgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCt                                                                  >  2:1685270/1‑150 (MQ=255)

                                                                                cgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCtt                                                                 <  1:1296758/150‑1 (MQ=255)

                                                                                   cTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc                                                              <  2:825552/150‑1 (MQ=255)

                                                                                       gATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCaa                                                          <  2:1080348/150‑1 (MQ=255)

                                                                                       gATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCaa                                                          <  2:835041/150‑1 (MQ=255)

                                                                                            ggCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACAt                                                     <  2:1938724/150‑1 (MQ=255)

                                                                                              cGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCt                                                   >  2:2005902/1‑150 (MQ=255)

                                                                                              cGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCt                                                   >  1:1791050/1‑150 (MQ=255)

                                                                                              cGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCt                                                   <  1:1924109/150‑1 (MQ=255)

                                                                                               gagaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCtt                                                  >  1:1417665/1‑150 (MQ=255)

                                                                                                agaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCttt                                                 <  2:126691/150‑1 (MQ=255)

                                                                                                  aaaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTaa                                               >  2:1595459/1‑150 (MQ=255)

                                                                                                  aaaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTaa                                               <  1:1388878/150‑1 (MQ=255)

                                                                                                  aaaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTaa                                               >  2:194967/1‑150 (MQ=255)

                                                                                                  aaaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTaa                                               >  2:195379/1‑150 (MQ=255)

                                                                                                  aaaTGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTaa                                               <  2:131726/150‑1 (MQ=255)

                                                                                                   cgaacaaCCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAAt                                              >  2:729115/7‑150 (MQ=255)

                                                                                                      gCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGtt                                           <  2:349797/150‑1 (MQ=255)

                                                                                                       ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTg                                          <  1:1656863/150‑1 (MQ=255)

                                                                                                       ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTg                                          <  2:1936114/150‑1 (MQ=255)

                                                                                                       ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTg                                          <  2:1351753/150‑1 (MQ=255)

                                                                                                       ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTg                                          >  1:1245012/1‑150 (MQ=255)

                                                                                                        cACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTgg                                         >  1:497730/1‑150 (MQ=255)

                                                                                                        cACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTgg                                         <  2:65976/150‑1 (MQ=255)

                                                                                                        cACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTgg                                         <  1:654216/150‑1 (MQ=255)

                                                                                                         aCCCGCGCGCGGGTTGTTCGGCATCAGAACCCGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCGTTGTTGCCGCGCTCTTCTGTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTTATGGTggg                                        >  2:460450/1‑150 (MQ=255)

                                                                                                           ccGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGgac                                      >  1:49828/1‑150 (MQ=255)

                                                                                                           ccGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCATTAATGTTGGgac                                      >  1:328924/1‑150 (MQ=255)

                                                                                                               gcgcgGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGAcc                                  <  1:1493996/150‑1 (MQ=255)

                                                                                                                         ttCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTg                        <  2:336372/150‑1 (MQ=255)

                                                                                                                         ttCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTg                        >  1:1093993/1‑150 (MQ=255)

                                                                                                                          tCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGt                       <  1:1685270/150‑1 (MQ=255)

                                                                                                                          tCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGt                       <  1:73442/150‑1 (MQ=255)

                                                                                                                             gCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGt                    <  1:862345/150‑1 (MQ=255)

                                                                                                                               aTCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTa                  <  2:633815/150‑1 (MQ=255)

                                                                                                                                     aCCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAggg            <  1:1704836/150‑1 (MQ=255)

                                                                                                                                     aCCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAggg            <  1:1004268/150‑1 (MQ=255)

                                                                                                                                      ccAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGa           >  2:1211298/1‑150 (MQ=255)

                                                                                                                                      ccAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGa           >  1:65322/1‑150 (MQ=255)

                                                                                                                                       cAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaa          <  2:891264/150‑1 (MQ=255)

                                                                                                                                        aGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaaa         >  2:1591005/1‑150 (MQ=255)

                                                                                                                                         gATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAat        >  1:2103141/1‑150 (MQ=255)

                                                                                                                                              cTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTcg   <  2:1055035/150‑1 (MQ=255)

                                                                                                                                               tACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTcgc  >  2:378662/1‑150 (MQ=255)

                                                                                                                                               tACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTcgc  <  2:1144173/150‑1 (MQ=255)

                                                                                                                                               tACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTcgc  <  1:2005902/150‑1 (MQ=255)

GCCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAATATTCGC  >  NC_000913/1143852‑1144144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`