Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,569 | 0 | C | G | 30.1% | 149.3 / 34.3 | 83 | intergenic (+35/‑161) | hrpB/mrcB | putative ATP‑dependent RNA helicase HrpB/peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB |
Reads supporting (aligned to +/- strand): ref base C (29/29); new base G (0/25); total (29/54) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.06e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
AAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTG > NC_000913/164421‑164710 | aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc > 1:450068/1‑149 (MQ=255) aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc < 1:698027/149‑1 (MQ=255) aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc > 2:1050182/1‑149 (MQ=255) ggAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 2:273188/149‑1 (MQ=255) gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgggg < 1:354821/149‑1 (MQ=255) aGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt > 1:1043256/1‑149 (MQ=255) gcttACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGTACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTATCCCTGTGGGAGAGGGCCGGGGTg < 2:1033978/146‑1 (MQ=255) gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTg > 1:1513989/1‑149 (MQ=255) gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTg < 1:813383/149‑1 (MQ=255) gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTg > 2:28612/1‑149 (MQ=255) cGTACCGTGAGGTGCAAAAAGAGATGCAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAC‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa < 2:1265746/149‑1 (MQ=255) gTACGGTGTGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 1:550717/149‑1 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATCCTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 2:1975523/149‑1 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 1:1505776/149‑1 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 2:331198/149‑1 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 1:213792/149‑1 (MQ=255) gTACCGTGAGGTGAAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCGGGCCGGACGACCCGGA‑AAATACTGCACCGACGCGACGGACTAAAAAGTATTCTTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 1:550730/149‑1 (MQ=39) tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg > 2:1842447/1‑149 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGc > 2:973531/1‑149 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGAAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGc > 1:1468256/1‑149 (MQ=255) gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg > 2:1413109/1‑149 (MQ=255) gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGCCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg > 2:2014494/1‑149 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATTCTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCa > 2:320965/1‑149 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCa > 1:1364871/1‑149 (MQ=255) aacgacaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGAGGCGAGGGACTAAAAAGTATCCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACg < 2:1249123/143‑1 (MQ=38) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACg > 2:669674/1‑149 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:166398/1‑149 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:921891/1‑149 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:1306677/1‑149 (MQ=255) aaaGAGATGAAAGGGCGTTATCCAAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg > 1:719629/1‑149 (MQ=255) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt > 1:47066/1‑149 (MQ=255) agagATCCAAGGGCGTTAACCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATAGTTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGt‑t < 2:2083344/149‑1 (MQ=38) ataTGAATGGGCGTTATCCCAAACTTGTCTGGCCGGACGACCCGGA‑AAATACTGCACCGCCGCGCCGGACGAAAACGTTTTCTTAT‑GGTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGT‑tca < 2:166398/147‑1 (MQ=25) agaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGtgga > 2:1134151/1‑146 (MQ=255) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtggcccc > 2:1785939/1‑143 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑tgacccg > 2:1475562/1‑148 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑tgacccac > 1:2130270/1‑147 (MQ=255) atgggCTTTATCCAAAACTTGTCTGGCGGGACGCCCGGTC‑AAATACTCCACCGACGCGACGGACTAAAATTTATT‑GTAATGGTTGTTTTTCTCCCTCTCCCTGTGGTTTTGGGCGGGGTTGTGGGCATCAGCGCGCACGT‑tcaccctca < 2:1865866/147‑1 (MQ=255) gggCGTTATCCCAAACATGTCTGGCCGGGCGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑gcacccgcacc > 1:1837934/1‑149 (MQ=255) gTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑GGACCCACCCCCaaa > 2:1184534/1‑149 (MQ=38) aTCCCAAACATTTCGGGCCGGACG‑CCCGGCAAAATCCTGCACCGACGCGACGGCCGAAAACTTTTACGTAA‑GGTTGTTTTTCCCCCCCTCCGTGTGGGAGCGGGCGGGGTTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAAccc < 2:1382279/149‑1 (MQ=255) aTCCCAAACATGTCTGGCGGGACGACCCGTA‑AATTACTCCACCGCGGCGCCTGACTAAAAATTTTTCTTTT‑TTTTGTTTTTCCCCCCCCCGGTGGGGTTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAAccc < 2:544305/149‑1 (MQ=255) cccAAACATGTCGGGCGGGACGACCCGGA‑AAATACTGCACCGACGCGACGGACTAAAATTTATTCTTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCctc < 2:1937136/149‑1 (MQ=37) cAAACTTGTGGGGCGGGCCGACCCGCA‑AATTAGTGCACCGACGCGCGGGACTAAAAATTATTGGTAT‑GTTTGTTTTTCCCCCTCTCCGTGGGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCtctc < 2:355189/149‑1 (MQ=255) cAAACAGGTCGGGCCGCACCCCCCGAA‑AAAAACTCCCCCGACGCGACGGACTAAAAATTATTCTTAT‑GTTTGGTTTTCCCCCTCCCCGTGGGGTAGCGGGCGGGGGGGTGGCCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCtctc < 2:244395/149‑1 (MQ=255) aaccATGTCTGGCCGTACCCCCCGGC‑AAATACTGCACCGCCGCCCCGTACTAAAAATTATTCGTAA‑GTTGGGTTTTCGCCCACTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTcc < 2:942493/146‑1 (MQ=255) aaCATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGT‑GGGCCCccacccccacccacaacc > 1:1862891/1‑143 (MQ=37) ggcggggCGGGCCGACCCGCA‑AACTACGCCACCGACGCGCCGCACTAAAATTTATTCTTAC‑GGTTGGTTTCCCCCCTCCCCCTGGGGGCGGGGGCGGGGGTGGGGCCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCaa < 1:2003351/144‑1 (MQ=255) tCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCGGCGCGCACGG‑TCACCCGCCCCCGGAGCCGCTCCCTCACgg > 2:1312122/1‑149 (MQ=37) cTGGCCGGCCCCCCCGGC‑AAATACGGCCCCGCCGCGCCGGACTAAAAAGTATACGTAT‑GGTTGGGTTGCTCCCTCCCCCTGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAggg < 2:1084543/149‑1 (MQ=255) gCCGGACGCCCCGGA‑AACTACTGCACCGAGGCGCCGGACTAAAAAGTATTCTAAC‑GGTTGGTTGACCCCCACCCCCGGGGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:236371/149‑1 (MQ=255) gCCGGACGCCCCGCA‑AAATACGGCCCCGCCGCGAGGCACTAAAAATTATTCGAAA‑GTTTGGTTTTCCCCCCCCCCCTGTGGGTGTGGGCGGGGTTGCGGCCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 1:1506663/149‑1 (MQ=255) ccGGACGACCCCGA‑AACTACTGCACCGACGCGCCGCACGAAAAAGTATCCGTAA‑GTTTGGTTTCCTCCCTCGCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTTGAGGGagag < 2:1413724/149‑1 (MQ=37) accacCCGGC‑AAATCCTCCCCCGACGCGACGCACGAAAAATTATTCTTAC‑GGTTGTTTTACCCCCCCACCGTGTGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGa < 1:1568941/146‑1 (MQ=255) acCCGCA‑AAATACTCCCCCCCCGCGCCGTACAAAAAAGTATTCTTAT‑GGTGGTTTTCCCCCCCCCCCCGGGGGGCGCGGGCCGGGGGGCGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCg < 2:562866/149‑1 (MQ=255) cccgcaaaaTACGCCACCGCCGCGCCGGCCTAAAATTTTTTGGTAT‑GGTTGTTTTTCCCCCTCTCCGTGGGGGTGGGGGCGGGGGTGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGa < 1:1732796/143‑1 (MQ=255) aaaGACTGCACCGACGCGACGGACCAAAAAGTCTTCGTAA‑GGTTGGTTTTCTCCCACTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCACACCCTAACCCTCTACCTCAAGGGATATGGGAGCGATCGTGc < 2:2056087/149‑1 (MQ=37) tACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑GGCCCCCCAACCAAACCCCCTCCCGCACGGGGGAGGGGGCCGCTCGAGcgca > 1:2092573/1‑149 (MQ=25) ccctccgcgacgTAATAAAATTTTTTCTTAT‑GGTTGGTTTTCCCCCTCCCCGTGGGGGTGTGGGCGGGGTTGTGGCCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:638020/144‑1 (MQ=255) ccccacgcgccgGACTAAAATGTTTTCGTAA‑GTTTGTTTTTCTCCCTCCCCCTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:526290/145‑1 (MQ=255) aCCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGG‑GCCCCCCAACCCAAACCCACACCCGCAAGGGGGAGGGGCCCGCTCGCGCCCCAAtgtt > 2:2054880/1‑149 (MQ=21) aCCGACGCGACGGAAGAAAATGTTTTCTTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:1864433/149‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGG‑GCCCCCTCCCCCGAACCCACTCCCGCGAGGGGGCGGGGCCCCAGCCAGCGCACAATTTg > 2:1358849/1‑149 (MQ=21) cgccgcgaggGACTAAAAATTTTTGTTAT‑GGTTGGTTTTCCCCCTCCCCGGGGGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:548003/146‑1 (MQ=255) gcgacgGACTAAAAAGTTTTCGTTA‑GGTTGGTTTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 2:1653948/149‑1 (MQ=255) acggactAAAATGTTTTCTTAT‑GTTTGGTTTTCGCCCACTCCGGGTGGGAGTGGGCCGGGGGGCGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat < 2:1364871/149‑1 (MQ=255) aaattttATTCGTAT‑GGTTGGTTTTCACCCTCTCCCTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc < 1:2014494/143‑1 (MQ=38) aaaaaGTTTTCTTAT‑GGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc < 2:450068/149‑1 (MQ=38) aaGTATTGTTAT‑GGTTGTTTTTCTCCCTCCCCGTGGGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGG‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGt < 2:1306677/149‑1 (MQ=255) aTTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTg < 2:1169680/149‑1 (MQ=255) atCGAAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGTTGAGGGCATCAGCCCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 1:2115819/148‑1 (MQ=255) atggttggttTTCTCACACGCCCTGGGGCAGTGGGCGGGGGGGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTGTGAGAGATATCTTCTACTGTCTTGTAACag < 2:1406442/147‑1 (MQ=255) tggttgtttTTCTCCCACTCCCTTTGGTAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 2:112222/148‑1 (MQ=255) tggttggttATCCCCCTCACCCTGTGGGATCGGGCCGGGGTGAGGCCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:2085287/148‑1 (MQ=255) agttttgttTTCCCCCTCGCCGTGGGGGTGGGGGCGGGGGTGTGGCCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga < 1:1031121/143‑1 (MQ=255) ggttgtttTTCTCCCTCTCCCTGTGGTTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:1511225/149‑1 (MQ=255) ggttggttTACCCCCTCCCCGTGTGGGCGTGGGCGGGGGTGCGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:1043256/149‑1 (MQ=255) tttCTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 2:1779887/149‑1 (MQ=255) ccctctccctGTGGGTGTGGGGGGGGGTGTGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 1:1109585/149‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑GGACCCCCAACCAAAACCCCACCCGCAAGGGCGTGGGGTCCCCTCGAGCACCAATTTTGAGAGATATATTATTCAGTCTTTTTACAGAAAAAAAAAAAATAgg > 2:1554526/1‑149 (MQ=16) tccctGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCt < 2:1989918/149‑1 (MQ=255) ccggtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt < 2:1468256/146‑1 (MQ=255) tgtgggtgtgggCGGGGGTGCGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg < 1:1475562/149‑1 (MQ=255) ggtgtgggCGGGGGTGTGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc < 2:1797774/144‑1 (MQ=255) ggAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc < 2:213210/149‑1 (MQ=255) agagGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCCACCGCCACCC‑AACCCACACCCCCAAGGGAGGGGGGGCCCATCGGGCACCAATTTTGGGAGATAAATTGTTATGGTGTGTAAGCGAAGAACAGAAAAAAGGGCgttttggcccg > 2:684559/1‑142 (MQ=255) | AAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAA‑GGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGT‑TCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTG > NC_000913/164421‑164710 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |