Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,049,443 C→A R101L (CGC→CTC)  gfcA ← threonine‑rich inner membrane protein GfcA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,049,4430CA100.0% 380.3 / NA 103R101L (CGC→CTC) gfcAthreonine‑rich inner membrane protein GfcA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (46/57);  total (46/57)

Only 100 of 103 total aligned reads displayed.

AAATAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGCCAGCAGCCACGCCACC  >  NC_000913/1049297‑1049588
                                                                                                                                                  |                                                                                                                                                 
aaaTAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAgt                                                                                                                                                 >  1:49974/1‑149 (MQ=255)
aaaTAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGGATTAGAgt                                                                                                                                                 >  2:1435183/1‑149 (MQ=255)
  aTAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAgtgt                                                                                                                                               <  2:2008424/149‑1 (MQ=255)
          aaaaTCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACg                                                                                                                                       >  2:1737023/1‑149 (MQ=255)
           aaaTCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACgt                                                                                                                                      >  2:660794/1‑149 (MQ=255)
            aaTCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACgtg                                                                                                                                     <  2:1201971/149‑1 (MQ=255)
              tCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACgtggt                                                                                                                                   <  1:1427900/149‑1 (MQ=255)
                gATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGtggtgg                                                                                                                                 <  2:887323/149‑1 (MQ=255)
                gATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGtggtgg                                                                                                                                 <  2:1313254/149‑1 (MQ=255)
                 aTAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTggtggt                                                                                                                                >  2:1005068/1‑149 (MQ=255)
                  tAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGtt                                                                                                                               >  2:562584/1‑149 (MQ=255)
                   aaaaTAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTg                                                                                                                              <  1:250687/149‑1 (MQ=255)
                   aaaaTAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCAATTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTg                                                                                                                              <  2:488608/149‑1 (MQ=255)
                    aaaTAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGa                                                                                                                             >  1:1208025/1‑149 (MQ=255)
                    aaaTAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGa                                                                                                                             <  1:2109845/149‑1 (MQ=255)
                      aTAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAgg                                                                                                                           <  2:923020/149‑1 (MQ=255)
                      aTAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAgg                                                                                                                           >  1:346720/1‑149 (MQ=255)
                       tAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGgt                                                                                                                          >  1:1309784/1‑149 (MQ=255)
                        aaGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGgtg                                                                                                                         <  2:175453/149‑1 (MQ=255)
                           agGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTa                                                                                                                      <  1:2060171/149‑1 (MQ=255)
                           agGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTa                                                                                                                      <  2:833684/149‑1 (MQ=255)
                           agGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTa                                                                                                                      >  1:1395432/1‑149 (MQ=255)
                            gggCGCACCATTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTAt                                                                                                                     <  1:1472589/149‑1 (MQ=255)
                                 cACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGaa                                                                                                                <  1:1405749/149‑1 (MQ=255)
                                  aCCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAAc                                                                                                               <  2:1189642/149‑1 (MQ=255)
                                    cTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCg                                                                                                             >  2:481765/1‑149 (MQ=255)
                                     tttAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGc                                                                                                            <  2:533271/149‑1 (MQ=255)
                                       tAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCa                                                                                                          <  1:535868/149‑1 (MQ=255)
                                       tAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCa                                                                                                          <  1:639528/149‑1 (MQ=255)
                                       tAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCa                                                                                                          >  1:1815404/1‑149 (MQ=255)
                                         aTCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATc                                                                                                        >  1:613959/1‑149 (MQ=255)
                                          tCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCg                                                                                                       >  2:869831/1‑149 (MQ=255)
                                             ttGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCa                                                                                                    <  2:1931322/149‑1 (MQ=255)
                                              tGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCAc                                                                                                   >  1:1517140/1‑149 (MQ=255)
                                              tGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCAc                                                                                                   <  2:340450/149‑1 (MQ=255)
                                                   gttaaattaaAAAATTGCAAACCACTAACCCCCGGTACTAATTCCGAACCCGGTTTAAATTAAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCa                                                                                              <  2:1315701/145‑1 (MQ=255)
                                                   ggTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCa                                                                                              <  1:2072723/149‑1 (MQ=255)
                                                    gTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCAt                                                                                             <  2:493832/149‑1 (MQ=255)
                                                      taattaaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCAttt                                                                                           <  2:840356/149‑1 (MQ=255)
                                                        attaaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTtgc                                                                                         >  1:406814/1‑149 (MQ=255)
                                                        attaaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTtgc                                                                                         <  1:70871/149‑1 (MQ=255)
                                                        attaaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTtgc                                                                                         >  1:406718/1‑149 (MQ=255)
                                                         ttaaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACCGTCATTtgct                                                                                        >  2:1291634/1‑149 (MQ=255)
                                                         ttaaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACCGTCATTtgct                                                                                        >  2:1291557/1‑149 (MQ=255)
                                                          taaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTtgctg                                                                                       >  1:814228/1‑149 (MQ=255)
                                                          taaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTtgctg                                                                                       >  2:1065082/1‑149 (MQ=255)
                                                          taaAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTtgctg                                                                                       <  1:1622358/149‑1 (MQ=255)
                                                             aaaaaTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGcaa                                                                                    >  1:785654/1‑149 (MQ=255)
                                                             aaaaaTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGcaa                                                                                    >  1:1449044/1‑149 (MQ=255)
                                                              aaaaTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGcaac                                                                                   >  2:809311/1‑149 (MQ=255)
                                                              aaaaTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCAGTTGCTGcaac                                                                                   >  2:714142/1‑149 (MQ=255)
                                                                aaTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGcaacaa                                                                                 <  1:1153882/149‑1 (MQ=255)
                                                                aaTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGcaacaa                                                                                 >  1:149727/1‑149 (MQ=255)
                                                                 aTGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCaacaac                                                                                <  1:505350/149‑1 (MQ=255)
                                                                  tGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACt                                                                               <  1:993855/149‑1 (MQ=255)
                                                                  tGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACt                                                                               >  1:238008/1‑149 (MQ=255)
                                                                   gCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTg                                                                              <  2:878197/149‑1 (MQ=255)
                                                                   gCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTg                                                                              <  2:878168/149‑1 (MQ=255)
                                                                      aaTCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCa                                                                           <  2:613959/149‑1 (MQ=255)
                                                                             aaaGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGc                                                                    >  2:428940/1‑149 (MQ=255)
                                                                              aaGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGcc                                                                   <  2:1371176/149‑1 (MQ=255)
                                                                                 cccGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCcaca                                                                <  2:1475408/149‑1 (MQ=255)
                                                                                 cccGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCcaca                                                                <  1:232502/149‑1 (MQ=255)
                                                                                      tACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAAc                                                           >  1:2075818/1‑149 (MQ=255)
                                                                                      tACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAAc                                                           >  2:1032126/1‑149 (MQ=255)
                                                                                       aCGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACa                                                          >  2:1909113/1‑149 (MQ=255)
                                                                                       aCGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACa                                                          >  2:1909763/1‑149 (MQ=255)
                                                                                        cGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAg                                                         >  1:1173974/1‑149 (MQ=255)
                                                                                            tatCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGt                                                     <  2:515480/149‑1 (MQ=255)
                                                                                              tCGCAACCGGGTATCATTTAACTTTATTTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAAATGCCGTTg                                                   <  2:534877/149‑1 (MQ=255)
                                                                                                gCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGcc                                                 <  1:1796049/149‑1 (MQ=255)
                                                                                                 cAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCca                                                >  2:1997425/1‑149 (MQ=255)
                                                                                                      gggTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCg                                           >  2:30546/1‑149 (MQ=255)
                                                                                                       ggTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGa                                          >  2:1182895/1‑149 (MQ=255)
                                                                                                         tATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACa                                        <  2:1940727/149‑1 (MQ=255)
                                                                                                            cATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACGCCGACAACt                                     <  2:1183620/149‑1 (MQ=255)
                                                                                                            cATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACGCCGACAACt                                     <  2:1183797/149‑1 (MQ=255)
                                                                                                            cATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACt                                     >  2:1300354/1‑149 (MQ=255)
                                                                                                             aTTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTTGTGGACGTGGTGGTTGTGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTg                                    <  1:1435183/149‑1 (MQ=255)
                                                                                                              tttAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGt                                   >  1:1950557/1‑149 (MQ=255)
                                                                                                              tttAACTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGt                                   >  2:197609/1‑149 (MQ=255)
                                                                                                                 aaCTTTAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGc                                <  2:672743/149‑1 (MQ=255)
                                                                                                                     ttAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTg                            <  2:49974/149‑1 (MQ=255)
                                                                                                                     ttAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTg                            >  1:1619654/1‑149 (MQ=255)
                                                                                                                     ttAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTg                            <  1:742616/149‑1 (MQ=255)
                                                                                                                     ttAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTg                            >  2:1061200/1‑149 (MQ=255)
                                                                                                                     ttAATTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTg                            >  1:1620457/1‑149 (MQ=255)
                                                                                                                        aTTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCg                         <  2:1090931/149‑1 (MQ=255)
                                                                                                                        aTTTCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCg                         <  2:1090913/149‑1 (MQ=255)
                                                                                                                          ttCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGAc                       <  2:1657639/149‑1 (MQ=255)
                                                                                                                          ttCGACGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGAc                       <  2:1657700/149‑1 (MQ=255)
                                                                                                                              aCGCGTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAgcg                   <  1:328338/149‑1 (MQ=255)
                                                                                                                                gcgTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGcc                 <  2:1696222/149‑1 (MQ=255)
                                                                                                                                  gTTAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGGCACGGCGACAgttgtcg               <  2:1208025/149‑7 (MQ=255)
                                                                                                                                   ttcaaGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCACCAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGCcagc              <  2:1309784/146‑1 (MQ=255)
                                                                                                                                   ttAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTAGCCGTGCCGACAGCGCcagc              >  2:764853/1‑149 (MQ=255)
                                                                                                                                    tAAAGCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCAACAACTGTCGCCGTGCCGACAGCGCcagca             <  2:346720/149‑1 (MQ=255)
                                                                                                                                        gCAGTATTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGCCAGCAgcca         <  1:972787/149‑1 (MQ=255)
                                                                                                                                             aTTAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGCCAGCAgccacgcca    >  2:562046/1‑149 (MQ=255)
                                                                                                                                               tAGAGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGCCAGCAGCCACGccacc  >  2:14741/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
AAATAGTGCGAAAATCGATAAAATAAGAGGGCGCACCTTTAATCCTTGAAAGGTTAATTAAAAAAATGCAAATCTCTAAAGCCCGGTACGAATATCGCAACCGGGTATCATTTAACTTTAATTTCGACGCGTTAAAGCAGTATTAGCGTGTAGTGGACGTGGTGGTTGAGGTGTTATGAGAACCGCCATCGCCACTGTCATTTGCTGCAACAACTGCCAACGTCGCCACACCAACAGCCGTTGCCACACCGACAACTGTCGCCGTGCCGACAGCGCCAGCAGCCACGCCACC  >  NC_000913/1049297‑1049588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: