BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,143,998	C→T	G124S (GGT→AGT)	rne ←	ribonuclease E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,143,998	0	C	T	99.2%	442.5 / NA	120	G124S (GGT→AGT)	rne	ribonuclease E
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (42/77); minor base A (1/0); total (43/77)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAA  >  NC_000913/1143853‑1144137

   gCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAg                                                                                                                                       >  1:438214/1‑149 (MQ=255)

   gCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAg                                                                                                                                       <  1:420402/149‑1 (MQ=255)

    cGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGa                                                                                                                                      <  2:214872/149‑1 (MQ=255)

    cGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGa                                                                                                                                      <  2:1790130/149‑1 (MQ=255)

      gTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACt                                                                                                                                    >  1:14726/1‑149 (MQ=255)

        gcgcACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGa                                                                                                                                  <  2:786986/149‑1 (MQ=255)

           cACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATaa                                                                                                                               >  2:1240715/1‑149 (MQ=255)

             cGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAg                                                                                                                             <  2:597198/149‑1 (MQ=255)

             cGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAg                                                                                                                             <  2:597258/149‑1 (MQ=255)

               aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATACAggt                                                                                                                           >  2:278796/1‑149 (MQ=255)

               aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAtgt                                                                                                                           <  1:471484/149‑1 (MQ=255)

               aTAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAggt                                                                                                                           >  1:1462014/1‑149 (MQ=255)

                tAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAggtg                                                                                                                          <  1:1370506/149‑1 (MQ=255)

                 aaGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAggtgg                                                                                                                         >  2:784794/1‑149 (MQ=255)

                 aaGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAggtgg                                                                                                                         <  1:101420/149‑1 (MQ=255)

                 aaGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAggtgg                                                                                                                         <  2:1341436/149‑1 (MQ=255)

                    ccccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTa                                                                                                                      >  1:1101951/1‑149 (MQ=255)

                    ccccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTa                                                                                                                      <  2:383184/149‑1 (MQ=255)

                    ccccATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTa                                                                                                                      >  1:1100215/1‑149 (MQ=255)

                          gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCcg                                                                                                                <  1:682965/149‑1 (MQ=255)

                          gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCcg                                                                                                                <  1:733731/149‑1 (MQ=255)

                          gCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCcg                                                                                                                <  1:1589924/149‑1 (MQ=255)

                           ccTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCcgc                                                                                                               >  1:773046/1‑149 (MQ=255)

                             ttCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCgcgc                                                                                                             >  2:1373060/1‑149 (MQ=255)

                             ttCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCgcgc                                                                                                             >  1:159600/1‑149 (MQ=255)

                             ttCCGGCAGTTCAAGGATAGCCAGAGCGTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACTGCTAGAAATGACACCCGCGCGCGGGTTGTTCGGCATCATAACCAGATAGCTACAAGCCAGACTGAGACATGTGGTTAATGCCgcgc                                                                                                             >  2:861003/1‑149 (MQ=255)

                              tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGcc                                                                                                            <  2:1151412/149‑1 (MQ=255)

                              tCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGcc                                                                                                            <  1:1477478/149‑1 (MQ=255)

                                cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCtt                                                                                                          <  2:2008525/149‑1 (MQ=255)

                                cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCtt                                                                                                          <  2:442761/149‑1 (MQ=255)

                                cGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCtt                                                                                                          <  1:1071912/149‑1 (MQ=255)

                                 ggCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCttt                                                                                                         <  1:1482672/149‑1 (MQ=255)

                                  gCAGTTCAAGGCTTGCCAGTGGTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGTCTGATAAAGGTGGTTAATGCCGCGCCTttg                                                                                                        >  1:1363010/1‑149 (MQ=255)

                                  gCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCTCGCCTttg                                                                                                        <  1:767631/149‑1 (MQ=255)

                                  gCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttg                                                                                                        <  1:1571068/149‑1 (MQ=255)

                                    aGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTttgtt                                                                                                      <  2:438214/149‑1 (MQ=255)

                                          aGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCGGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCcgcg                                                                                                >  2:1717289/1‑149 (MQ=255)

                                            gCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGGTAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGct                                                                                              >  2:1271844/1‑149 (MQ=255)

                                             cTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGctc                                                                                             >  2:719678/1‑149 (MQ=255)

                                               tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                                                                           <  2:1579247/149‑1 (MQ=255)

                                               tGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtctt                                                                                           <  2:13660/149‑1 (MQ=255)

                                                gCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCtcttc                                                                                          <  1:356102/149‑1 (MQ=255)

                                                    gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTa                                                                                      >  1:321792/1‑149 (MQ=255)

                                                    gTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTa                                                                                      <  1:312348/149‑1 (MQ=255)

                                                      gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATc                                                                                    <  2:630509/149‑1 (MQ=255)

                                                      gCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATc                                                                                    >  2:1473116/1‑149 (MQ=255)

                                                        ttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGa                                                                                  <  1:1703310/149‑1 (MQ=255)

                                                        ttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGa                                                                                  <  2:649141/149‑1 (MQ=255)

                                                        ttcttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGa                                                                                  <  2:799685/149‑1 (MQ=255)

                                                        ttcttTTAATTCGGTACGGTCGTCGCCTTCGAGACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATACAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGa                                                                                  <  2:1187478/149‑1 (MQ=255)

                                                          cttTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATc                                                                                <  2:1574461/149‑1 (MQ=255)

                                                              tAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGaa                                                                            <  1:534175/149‑1 (MQ=255)

                                                                aTTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACa                                                                          <  1:1900839/149‑1 (MQ=255)

                                                                 ttCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                                                                         >  2:1378011/1‑149 (MQ=255)

                                                                 ttCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACaa                                                                         >  1:1237108/1‑149 (MQ=255)

                                                                  tCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAAt                                                                        <  2:420445/149‑1 (MQ=255)

                                                                  tCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAAt                                                                        <  2:420545/149‑1 (MQ=255)

                                                                   cGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACAAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATg                                                                       <  1:261320/149‑1 (MQ=255)

                                                                     gTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGAc                                                                     >  2:2103615/1‑149 (MQ=255)

                                                                      tACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACt                                                                    >  2:1191564/1‑149 (MQ=255)

                                                                        cGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTc                                                                  <  2:611951/149‑1 (MQ=255)

                                                                         ggTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTcc                                                                 <  2:297777/149‑1 (MQ=255)

                                                                         ggTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTcc                                                                 <  1:689950/149‑1 (MQ=255)

                                                                          gtcgtcGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTAGGCATCAGAACCAGATAGCTACTCGCCAGACTGATACAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCt                                                                <  1:1803640/149‑1 (MQ=255)

                                                                            cgtcgCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGa                                                              <  1:1240715/149‑1 (MQ=255)

                                                                                gCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCtt                                                          <  2:1949381/149‑1 (MQ=255)

                                                                                 ccTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTc                                                         >  1:527374/1‑149 (MQ=255)

                                                                                   ttCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc                                                       >  2:1808091/1‑149 (MQ=255)

                                                                                   ttCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc                                                       <  2:1100215/149‑1 (MQ=255)

                                                                                   ttCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc                                                       <  2:1101951/149‑1 (MQ=255)

                                                                                   ttCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCAc                                                       >  1:293729/1‑149 (MQ=255)

                                                                                      gATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCa                                                    <  2:1500289/149‑1 (MQ=255)

                                                                                        tACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAac                                                  >  2:471481/1‑149 (MQ=255)

                                                                                         aCGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAaca                                                 >  2:167982/1‑149 (MQ=255)

                                                                                         aCGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAaca                                                 <  2:1554814/149‑1 (MQ=255)

                                                                                             cGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATc                                             <  2:1446212/149‑1 (MQ=255)

                                                                                              gagaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCt                                            <  1:1808091/149‑1 (MQ=255)

                                                                                               agaAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCtt                                           <  2:863453/149‑1 (MQ=255)

                                                                                                     gCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGt                                     <  1:1496039/149‑1 (MQ=255)

                                                                                                      ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGtt                                    >  1:987087/1‑149 (MQ=255)

                                                                                                      ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGtt                                    <  1:320963/149‑1 (MQ=255)

                                                                                                      ccACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCG‑CAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTg                                   <  2:577154/149‑1 (MQ=255)

                                                                                                      ccACCCGCGCGCGGGTAGTTCGGCAACAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGGTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGtt                                    <  2:1124485/149‑1 (MQ=255)

                                                                                                          ccGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGga                                >  2:1394302/1‑149 (MQ=255)

                                                                                                            gcgcgcgGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGgacg                              <  1:2036058/149‑1 (MQ=255)

                                                                                                             cgcgcgGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGgacga                             <  1:614145/149‑1 (MQ=255)

                                                                                                             cgcgcgGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGgacga                             <  2:625635/149‑1 (MQ=255)

                                                                                                                gcgGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCa                          <  1:602156/149‑1 (MQ=255)

                                                                                                                gcgGGTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCa                          <  1:570348/149‑1 (MQ=255)

                                                                                                                  gggTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATg                        <  1:1244413/149‑1 (MQ=255)

                                                                                                                  gggTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATg                        <  1:1434811/149‑1 (MQ=255)

                                                                                                                  gggTTGTTCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATg                        <  2:536366/149‑1 (MQ=255)

                                                                                                                         tCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTg                 >  1:894792/1‑149 (MQ=255)

                                                                                                                         tCGGCATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTg                 >  1:894822/1‑149 (MQ=255)

                                                                                                                             cATCAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGt             >  2:1802357/1‑149 (MQ=255)

                                                                                                                                cAGAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAg          >  2:1721294/1‑149 (MQ=255)

                                                                                                                                  gAACCAGATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCa        >  2:1184433/1‑149 (MQ=255)

                                                                                                                                        gATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaaa  <  2:321792/149‑1 (MQ=255)

                                                                                                                                        gATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaaa  <  2:1462014/149‑1 (MQ=255)

                                                                                                                                        gATAGCTACTCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGaaa  >  1:1049053/1‑149 (MQ=255)

CCAGCGGTGCGCACGATAAGCCCCATGCCTTCCGGCAGTTCAAGGCTTGCCAGTGCTTCTTTTAATTCGGTACGGTCGTCGCCTTCGATACGGCGAGAAATGCCACCCGCGCGCGGGTTGTTCGGCATCAGAACCAGATAGCTACCCGCCAGACTGATAAAGGTGGTTAATGCCGCGCCTTTGTTGCCGCGCTCTTCTTTATCGATCTGAACAATGACTTCCTGACCTTCACGCAACACATCTTTAATGTTGGGACGACCATGAGCACTGTAGTTAGCAGGGAAA  >  NC_000913/1143853‑1144137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`