Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,917,345	G→A	G763G (GGG→GGA)	barA →	sensory histidine kinase BarA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,917,345	0	G	A	100.0%	190.7 / NA	52	G763G (GGG→GGA)	barA	sensory histidine kinase BarA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/34);  total (18/34)

TCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGAC  >  NC_000913/2917200‑2917489                                                                                                                                                  |                                                                                                                                                 tCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGTCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATg                                                                                                                                               <  1:701758/149‑1 (MQ=255)            atTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCTCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCt                                                                                                                                    <  1:1964973/149‑1 (MQ=255)            atTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCt                                                                                                                                    <  1:1965028/149‑1 (MQ=255)             ttCTAATGCCGGACATGGATGGCATTCGGGCATGCGAAATCATCCACCAACTCCCGCATCAGCAACAAACGCCTGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGATGCTTGGCGCAGGAATGAGCGATTATCTg                                                                                                                                   <  2:516516/149‑1 (MQ=255)             ttCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTg                                                                                                                                   <  1:1847344/149‑1 (MQ=255)                aaaTGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCg                                                                                                                                <  1:325932/149‑1 (MQ=255)                  aTGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGaa                                                                                                                              >  1:1506880/1‑149 (MQ=255)                       ggACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGa                                                                                                                         <  2:480766/149‑1 (MQ=255)                                tggCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGaagaag                                                                                                                >  2:154335/1‑149 (MQ=255)                                    aTTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCg                                                                                                            >  2:526579/1‑149 (MQ=255)                                      tCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGAt                                                                                                          <  1:1440340/149‑1 (MQ=255)                                        gggCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTg                                                                                                        <  1:2069736/149‑1 (MQ=255)                                                 aaCTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttg                                                                                               <  1:1691115/149‑1 (MQ=255)                                                 aaCTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttg                                                                                               <  1:816735/149‑1 (MQ=255)                                                  aCTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGTAGAGCGATTGCATAATttgt                                                                                              >  2:1258533/1‑149 (MQ=255)                                                   cTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgtt                                                                                             <  1:2108972/149‑1 (MQ=255)                                                     cATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCGGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGAGTGCATAATttgttgt                                                                                           <  2:1175549/149‑1 (MQ=255)                                                      aTCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgtt                                                                                          <  2:72581/149‑1 (MQ=255)                                                       tCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                         <  2:832290/149‑1 (MQ=255)                                                       tCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                         >  1:790080/1‑149 (MQ=255)                                                       tCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATttgttgttg                                                                                         >  2:1522892/1‑149 (MQ=255)                                                            caACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTa                                                                                    >  1:551262/1‑149 (MQ=255)                                                            caACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGAATGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTa                                                                                    >  1:551283/1‑149 (MQ=255)                                                                 cccGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGc                                                                               >  1:456516/1‑149 (MQ=255)                                                                         aGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGc                                                                       <  1:1609115/149‑1 (MQ=255)                                                                              caaACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTAt                                                                  <  1:1374179/149‑1 (MQ=255)                                                                                  cGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTcc                                                              >  2:1865241/1‑149 (MQ=255)                                                                                     cGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct                                                           <  1:551265/149‑1 (MQ=255)                                                                                     cGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCctct                                                           <  2:1506880/149‑1 (MQ=255)                                                                                       gTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcg                                                         <  1:951741/149‑1 (MQ=255)                                                                                       gTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcg                                                         <  1:154335/149‑1 (MQ=255)                                                                                        ttATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTcgc                                                        <  2:463356/149‑1 (MQ=255)                                                                                                      gcgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGa                                          <  1:271618/149‑1 (MQ=255)                                                                                                       cgcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGaa                                         <  1:218156/149‑1 (MQ=255)                                                                                                        gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAg                                        >  1:928837/1‑149 (MQ=255)                                                                                                        gcATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAg                                        <  2:2034281/149‑1 (MQ=255)                                                                                                           tGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCa                                     <  1:583267/149‑1 (MQ=255)                                                                                                             cAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAAc                                   <  2:1928393/149‑1 (MQ=255)                                                                                                                   ccGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAAtt                             >  2:1316798/1‑149 (MQ=255)                                                                                                                   ccGGGCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAAtt                             <  1:733080/149‑1 (MQ=255)                                                                                                                       gCAAAAAGAGAAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTgtgg                         <  2:1006644/149‑1 (MQ=255)                                                                                                                              gagaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAAccc                  >  1:1059812/1‑149 (MQ=255)                                                                                                                               agaAGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCg                 >  2:2057481/1‑149 (MQ=255)                                                                                                                                 aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGaa               <  1:1522892/149‑1 (MQ=255)                                                                                                                                 aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGaa               <  2:2020528/149‑1 (MQ=255)                                                                                                                                 aaGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGaa               <  1:1052919/149‑1 (MQ=255)                                                                                                                                  aGCTGCTTGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAAt              >  1:1460135/1‑149 (MQ=255)                                                                                                                                        ttGGCGCAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGAcc        <  1:1010152/149‑1 (MQ=255)                                                                                                                                            cgcAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCg    <  2:790080/149‑1 (MQ=255)                                                                                                                                              cAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGac  >  1:2014735/1‑149 (MQ=255)                                                                                                                                              cAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGac  >  1:1304548/1‑149 (MQ=255)                                                                                                                                              cAGGAATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCATCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGac  >  1:97632/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TCTTAATGGATATTCAAATGCCGGACATGGATGGCATTCGGGCCTGCGAACTCATCCACCAACTCCCGCATCAGCAACAAACGCCGGTTATCGCGGTAACGGCGCATGCAATGGCCGGGCAAAAAGAGAAGCTGCTTGGCGCAGGGATGAGCGATTATCTGGCGAAACCGATTGAAGAAGAGCGATTGCATAATTTGTTGTTGCGCTACAAGCCTGGCAGCGGTATTTCCTCTCGCGTCGTGACGCCCGAAGTCAACGAAATTGTGGTGAACCCGAATGCGACCCTCGAC  >  NC_000913/2917200‑2917489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑