Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	151,734	0	A	C	30.4%	120.0 / 24.4	79	V166V (GTT→GTG)	yadL	putative fimbrial protein YadL
Reads supporting (aligned to +/- strand):  ref base A (36/19);  new base C (0/24);  total (36/43)
Fisher's exact test for biased strand distribution p-value = 7.93e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTGCGGCTGTGGAA  >  NC_000913/151586‑151878                                                                                                                                                      |                                                                                                                                                 tGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATaaa                                                                                                                                                 >  1:2595463/1‑150 (MQ=255)    gTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGa                                                                                                                                              >  1:591749/1‑150 (MQ=255)     tGGTATGCATATTTAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGAAAATGTGGCTGGTGCAGAAAAATTACCCGCAGTGACATTTTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                                                             <  2:227910/150‑1 (MQ=255)     tGGGATGCATATATAGTTCCTTAATCTGTAAGTCTTTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACTTTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                                                             <  2:227811/150‑1 (MQ=255)     tGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGAc                                                                                                                                             >  1:258617/1‑150 (MQ=255)        gATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGc                                                                                                                                          >  1:2860986/1‑150 (MQ=255)           gaatatatAGTTCCTTAATCTTTAAGTATGTTTTTTATAGTTTACTTTAAATGGGGCTGGTGCAGAAAACTTACCCGCTGTGAATTTTTTTACGTTTTTTTTTTTTT‑CCACTACCCGGGGTGCACTCATCGTATAATACACGACCGCAtt                                                                                                                                       <  2:1324585/148‑1 (MQ=255)                 ataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAAATTACCCGCACTGACATGGTTTACGGTTTTATTTTTTT‑CCACGACCATGCGTGCACTCATCGGATAATAAACGACCGCAATGGCTGc                                                                                                                                 >  2:1659568/1‑150 (MQ=255)                     ttCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTTTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTg                                                                                                                             <  2:2208388/150‑1 (MQ=255)                      tCCTTAATCTTTAAGTCTGTTATTTATCGGTTACTGTAAATGTGGCTGGTGCATAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCCCGACCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTgg                                                                                                                            <  2:1084109/150‑1 (MQ=255)                      tCCTTAATCTGTAATTCTTTTTTTTATCGTTTACTGTAAAGGTGGCTGGTGCATAAAACTTACCCGCAGTCACTTTGTTTCCGGTTTTATTTTTTA‑CCACCACCAGGCGTGCACTCAGCGTAAAATAAACGACCGCATTGGCTGCAGTgg                                                                                                                            <  2:1084095/150‑1 (MQ=255)                      tCCTGAATCTTTACGTCTGTTAGTTATCGGTTACTTTAAATGTGGCTGGTGCTGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCCGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTgg                                                                                                                            <  2:2564262/150‑1 (MQ=255)                         tGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGgaga                                                                                                                         >  1:1559919/1‑150 (MQ=255)                          gAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAt                                                                                                                        >  2:1627445/1‑150 (MQ=255)                          gAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGAt                                                                                                                        >  1:2247030/1‑150 (MQ=255)                              cTGTAATTCTGTTAGTTATTGGTTACTTTAAATGTGGCTGTTGCATAAAACTTACCCGCCGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGCCCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGtt                                                                                                                    <  2:409564/150‑1 (MQ=255)                              cTGAAAGTCTTTAAGTTATCGGTTACTTTAAATGTGGCTGGTGCAGAAAACTCACCCCCAGTTACATTTTTTACGGTTTTATTTTTTA‑CCACGACCAGGCGTGCACCCATGGGAAAATAAACGACCGCATTGGCTGCAGTGGAGATTGtt                                                                                                                    <  2:409816/150‑1 (MQ=255)                               tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTA‑CCACGACCAGGCGTGCACTCAACGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTa                                                                                                                   <  2:2868333/150‑1 (MQ=255)                                         ttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACCTTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGa                                                                                                         >  2:1639279/1‑150 (MQ=255)                                         ttagttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGa                                                                                                         >  2:1635293/1‑150 (MQ=255)                                          tatttaTTGTTTACTTTAATTGTGGGTGGTGAATAAAACTAACCCGCAGTCAAATTTTTTACGGTTTTATTTTTTA‑CCACGACCCGGCGTGCACTCATCGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGAt                                                                                                        <  2:771175/147‑1 (MQ=255)                                          tagttaTAGGTTAATTTAAATGTGGCGGGTGCAGAAAACTTCCCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGAt                                                                                                        <  2:2594645/150‑1 (MQ=255)                                            gttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCAATGGCTGCAGTGGAGATTGTTACCTCTTGTGATGc                                                                                                      >  2:1110978/1‑150 (MQ=255)                                             ttaTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCt                                                                                                     >  2:1061586/1‑150 (MQ=255)                                               atcggTTACTGTAAATGTGGCTGGTGAATAAAACTTCCCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTgg                                                                                                   <  2:1941656/147‑1 (MQ=255)                                               ataGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGTTTTTATTTTTTT‑CCACGACCCGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTgg                                                                                                   <  2:1743879/150‑1 (MQ=255)                                                ttggTTACTTTAATGGGGGCTGGTGCACAAAACTTACCCGCTGTGAATTTTTTTACGGTTTTATTTTTTC‑CCACCACCCGGCGTGCACTCATGGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgt                                                                                                  <  1:1549896/148‑1 (MQ=255)                                                taGGTTACTTTAATTGTGGCTGGTGCAGAAAACTTCCCCGCAGTGACTTTGTTTAGGTTTTTATTTTTTC‑CCCCGACCAGGGGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgt                                                                                                  <  1:855382/150‑1 (MQ=255)                                                 tgtttACTGTAATTGGGGCGGGTGCACAAAACTAACCCGCAGTGACTTTGTTTAGGTTTTTATTTTTGT‑CCCCGCCCCGGCGTGCACCCAGCGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGgtg                                                                                                 <  1:1356614/147‑1 (MQ=255)                                                    ttACTGTAAATGTGGCTGTTGCATAAAACTTACCCGCAGTGACATTGTTTACGTTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTa                                                                                              <  2:1984355/150‑1 (MQ=255)                                                       ctttAATTGTGGCTGGTGCATAAAACTTACCCGCATTGACATTGTTTACGTTTTTATTTTTTC‑CCCCCACCAGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                           <  1:939419/147‑1 (MQ=255)                                                       ctttAAATGTGGCTGGGGCATAAAACTTCCCCGCTGTGACTTTGTTTACGTTTTTATTTTTTT‑CCACGACCCGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                           <  2:466221/147‑1 (MQ=255)                                                       cTGTAATTGTGGCGGGTGCATAAAACTTACCCGCAGTGACTTTTTTTACGTTTTTATTTTTTA‑CCACGACCTGGCGTGCACTCATCGTTTAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTacac                                                                                           <  1:2701155/150‑1 (MQ=255)                                                        tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTAcaca                                                                                          >  2:2377925/1‑150 (MQ=255)                                                         gTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGTTGTTACACAg                                                                                         >  2:1720823/1‑150 (MQ=255)                                                         gTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAg                                                                                         >  1:2561168/1‑150 (MQ=255)                                                            aaTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTa                                                                                      >  2:2126997/1‑150 (MQ=255)                                                              tgtgGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTaaa                                                                                    <  2:2052358/150‑1 (MQ=255)                                                                     ggTGCATAAAACTTACCCGCAGTTAATTTTTTTCCGTTTTTATTTTTTA‑CCCCCCCCTGGCGTGCACTCATCGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGtt                                                                             <  1:476472/150‑1 (MQ=255)                                                                     ggTGCATAAAACTCACCCGCTGTCACTTTGTTTACGTTTTTATTTTTTC‑CCCCCACCCGGCGTGCACACATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGtt                                                                             <  1:476403/150‑1 (MQ=255)                                                                      gTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTg                                                                            >  1:864571/1‑150 (MQ=255)                                                                         cAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCAc                                                                         >  1:1217309/1‑150 (MQ=255)                                                                          aGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACt                                                                        >  2:2139155/1‑150 (MQ=255)                                                                           gAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTc                                                                       >  2:1797556/1‑150 (MQ=255)                                                                            aaaaCTTACCCGCAGTTACTTTGTTTACGTTTTTATTTTTTA‑CCCCGACCCGCCGTGCACACATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTcc                                                                      <  2:1172837/150‑1 (MQ=255)                                                                             aaaCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTccc                                                                     >  1:105539/1‑150 (MQ=255)                                                                             aaaCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTccc                                                                     >  1:105522/1‑150 (MQ=255)                                                                                cTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGt                                                                  >  1:2163897/1‑150 (MQ=255)                                                                                     ccGCAGTGACATTGTTTCCGGTTTTATTTTTTC‑CCACGACCCGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCg                                                             <  2:1559919/150‑1 (MQ=255)                                                                                       gCAGTTACTTTGTTTACGGTTTTATTTTTTC‑CCACGACCAGGCGTGCACTCAGCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                                                           <  2:849056/150‑1 (MQ=255)                                                                                       gCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                                                           >  1:845433/1‑150 (MQ=255)                                                                                       ccAGTGACTTTTTTTAGGGTTTTATTTTTTA‑CCACTCCCCGGCGTGCACACATCGTATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGa                                                           <  2:849527/149‑1 (MQ=255)                                                                                           tGACATTGTTTAGGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTa                                                       <  2:2375804/150‑1 (MQ=255)                                                                                             actttttttACGGTTTTATTTTTTC‑CCACGACCCGGCGTGCACTCATCGTATAAAACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa                                                     <  1:2513687/144‑1 (MQ=255)                                                                                             actttGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACa                                                     <  2:2894402/147‑1 (MQ=255)                                                                                             aatttttttCCGTTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCCCTCCCTGTTGCCGGAGTTACa                                                     <  2:382006/144‑1 (MQ=255)                                                                                              cATTGTTTACGTTTTTATTTTTTT‑CCCCGACCTGGCGTGCACTCATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAg                                                    <  1:2332103/150‑1 (MQ=255)                                                                                               aTTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGt                                                   >  2:540067/1‑150 (MQ=255)                                                                                               aTTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGt                                                   >  2:540064/1‑150 (MQ=255)                                                                                               aTTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGt                                                   >  1:2409424/1‑150 (MQ=255)                                                                                               aTTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGt                                                   >  1:2409412/1‑150 (MQ=255)                                                                                                  gTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCt                                                >  2:1135528/1‑150 (MQ=255)                                                                                                   tttAGGGTTTTATTTTTTA‑CCACGACCAGGCGTGCACTCATCGGTTAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTc                                               <  1:1903784/150‑1 (MQ=255)                                                                                                     ttcGGTTTTTTTTTTTTACCACCACCAGGGGTGCACCCATCGTATAAACCACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTcc                                              <  1:1502569/148‑1 (MQ=255)                                                                                                     tccgtttttATTTTTTT‑CCCCGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTAATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCa                                             <  1:2635751/145‑1 (MQ=255)                                                                                                      aCGTTTTTTTTTTTTT‑CCCCGACCAGGCGTCCACTCTTGGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAg                                            <  1:1498622/150‑1 (MQ=255)                                                                                                         gTTTTATTTTTTA‑CCCCCACCCGGGGTCCACACATCGGATAAAAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACt                                         <  1:1098980/150‑1 (MQ=255)                                                                                                            ttATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCa                                      >  1:939076/1‑150 (MQ=255)                                                                                                            ttATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCa                                      >  1:2820425/1‑150 (MQ=255)                                                                                                             tATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCaa                                     >  2:2174033/1‑150 (MQ=255)                                                                                                                    ttgccACGCCCAGGGGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGa                               <  2:2595463/147‑1 (MQ=255)                                                                                                                     t‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACa                             >  1:2105540/1‑150 (MQ=255)                                                                                                                       ccACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAg                            <  1:2139155/150‑1 (MQ=255)                                                                                                                           gACCAGGCGTGCACACATCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACt                        <  1:501279/150‑1 (MQ=255)                                                                                                                            aCCAGGCGTGCACACAGCGTATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACtt                       <  1:1143770/150‑1 (MQ=255)                                                                                                                             ccAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTg                      >  1:1363838/1‑150 (MQ=255)                                                                                                                                ggCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCtt                   >  2:716432/1‑150 (MQ=255)                                                                                                                                 gCGTGCACTCATCGGATAATACACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCttt                  <  2:1868715/150‑1 (MQ=255)                                                                                                                                       aCTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTGc            >  1:657999/1‑150 (MQ=255)                                                                                                                                           aTCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTGCGGCt        >  2:1508342/1‑150 (MQ=255)                                                                                                                                                 taataaACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTGCGGCTGTGGaa  <  1:2119998/150‑1 (MQ=255)                                                                                                                                                 taataaACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTGCGGCTGTGGaa  <  2:187644/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 TGAGTGGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTT‑CCACGACCAGGCGTGCACTCATCGGATAATAAACGACCGCATTGGCTGCAGTGGAGATTGTTACCTCTTGTGATGCTGGTGTTACACAGCTAAATTGTGTTGCACTCCCTGTTGCCGGAGTTACAGTGCTCCAGACTTCAACGGCGACAGCACTTGCTTTATCTGCGGCTGTGGAA  >  NC_000913/151586‑151878

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑