Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913159,1010CA25.0% 348.5 / 37.0 140C9F (TGC→TTC) pcnBpoly(A) polymerase I
Reads supporting (aligned to +/- strand):  ref base C (81/24);  new base A (0/35);  total (81/59)
Fisher's exact test for biased strand distribution p-value = 1.80e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 140 total aligned reads displayed.

CGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGC  >  NC_000913/158953‑159249
                                                                                                                                                    |                                                                                                                                                     
cGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCa                                                                                                                                                      >  2:1569341/1‑150 (MQ=255)
cGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCa                                                                                                                                                      >  2:1569232/1‑150 (MQ=255)
       ttGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTa                                                                                                                                               <  1:1112442/150‑1 (MQ=255)
         gAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑Gc                                                                                                                                            <  1:533022/150‑1 (MQ=255)
             cTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACt                                                                                                                                        >  2:761469/1‑150 (MQ=255)
             cTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACt                                                                                                                                        >  2:309320/1‑150 (MQ=255)
              tGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCTTGCTGCTCACGGGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTc                                                                                                                                       <  2:1298401/150‑1 (MQ=255)
               gTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCg                                                                                                                                      >  2:2698419/1‑150 (MQ=255)
                tACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCgg                                                                                                                                     >  1:123580/1‑150 (MQ=255)
                tACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCgg                                                                                                                                     >  1:123584/1‑150 (MQ=255)
                tACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCgg                                                                                                                                     >  1:123654/1‑150 (MQ=255)
                 aCATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCggg                                                                                                                                    >  1:1811079/1‑150 (MQ=255)
                  cATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGt                                                                                                                                   >  2:1887437/1‑150 (MQ=255)
                  cATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGt                                                                                                                                   >  2:2303021/1‑150 (MQ=255)
                   aTTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTa                                                                                                                                  >  2:1962802/1‑150 (MQ=255)
                      aCCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTaaaa                                                                                                                               >  2:2264635/1‑150 (MQ=255)
                        cTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAAt                                                                                                                             >  2:1711782/1‑150 (MQ=255)
                          tgcgggCTTTTTCAGTGATTTGTTTGCGGTAAATAGCATGCTGCTCACGCGGTATCTCCGACCCCTGTGGCCGGGCGACTGCCTGTTCAGCCTCGATTTCGTCGCGGCTTAGCACATTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAg                                                                                                                           <  2:2603798/147‑1 (MQ=255)
                          tCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAg                                                                                                                           >  2:1092055/1‑150 (MQ=255)
                           cAGGGATTTTTCACTGATATCTTTGGGGTAAATAGAATGCTGCTCACGCGGGATCCCCGTCACCTGTGGACGGGCGACTCCCTGTTCAGCCTCGGTTTCCTCGCGGCTTAGCCCGTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGt                                                                                                                          <  2:1199953/150‑1 (MQ=255)
                            aGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTa                                                                                                                         >  2:2707818/1‑150 (MQ=255)
                            aGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTa                                                                                                                         >  2:2707883/1‑150 (MQ=255)
                             gggCATTTTCACTGATATCTTTGCGGGAAATAGCTTGCTGCCCACGCGGTATCACCGTCACCTGTGGAGGGGCGACTGCCTTTACAGCCTCGCTTTCCTCGCGGATTAGCACCTTGCGGCAAAATTTA‑GCGACTCGGGTAAAAATAGTac                                                                                                                        <  2:2622116/150‑1 (MQ=255)
                              ggCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTaca                                                                                                                       >  1:1856896/1‑150 (MQ=255)
                              ggCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTaca                                                                                                                       >  2:2425969/1‑150 (MQ=255)
                                  ttttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTc                                                                                                                   >  1:2494938/1‑150 (MQ=255)
                                  ttttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTc                                                                                                                   >  1:1279455/1‑150 (MQ=255)
                                   tttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCg                                                                                                                  >  2:899952/1‑150 (MQ=255)
                                   tttCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCg                                                                                                                  >  2:2872054/1‑150 (MQ=255)
                                    ttCACTTATATCTTGGCGGTAAATAGCATGCTGCTCCCGCGGTATCACCGTCACCTGTGGAGGGGCGACTGCCTTTTCAGCCTCGCTTTCCTCGGGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCgg                                                                                                                 <  1:2771609/150‑1 (MQ=255)
                                     tCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGt                                                                                                                >  1:2943094/1‑150 (MQ=255)
                                      cACTTATATCTTTGCGGTAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGAGGGGCGACTGCCTGTTCAGCCACGATTTCCTCGGGGATTAGCACCTGGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTa                                                                                                               <  2:867284/150‑1 (MQ=255)
                                      cACTTATATCTTTGCGGTAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTa                                                                                                               <  2:2705424/150‑1 (MQ=255)
                                        cTGATATCTTTGCGGTAAATAGCAGGCTGCTCACGCGGTTTCACCGTCACGTGTGGACGGGCGAGTGCGTGTTCAGCCTCGTTTTCCTCGCGGCTTTGAACCTTGCGGAAAAATTTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAgt                                                                                                             <  2:1052191/150‑1 (MQ=255)
                                         tGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAgtg                                                                                                            >  1:2044405/1‑150 (MQ=255)
                                          gATATCTTTGCGGTAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGAGGGGCGACTGCCTTTTCAGCCTCGCTTTCCTCGCGGATTAGCACCTTGCGGCAAAAATTC‑GCGACTCGGGTAAAAATAGTACACCTCGGTAgtgt                                                                                                           <  2:1667224/150‑1 (MQ=255)
                                             atCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCaa                                                                                                        >  2:1251003/1‑150 (MQ=255)
                                                   gCGGTAAATTGAATGCGGCTCACGCGGTATCACCGACACCTGTGGAGGGGCGACTGCCTGTTCACCCTCGCTTTCCTCGCGGCTTACCACCTTGCGGCAAAAATAC‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAAcatca                                                                                                  <  1:2425969/150‑1 (MQ=255)
                                                   gCGGTAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAAcatca                                                                                                  <  2:426969/150‑1 (MQ=255)
                                                      ggAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTc                                                                                               <  2:2171194/150‑1 (MQ=255)
                                                          aTAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGAGGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGa                                                                                           <  1:2772938/150‑1 (MQ=255)
                                                          aTAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGa                                                                                           >  2:2500836/1‑150 (MQ=255)
                                                          aTAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGa                                                                                           >  2:2500846/1‑150 (MQ=255)
                                                             gCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGGAAAAATAGTACACATCGGTAGTGTCAAACATCATTCTGGACaa                                                                                        >  2:1940350/1‑150 (MQ=255)
                                                                 gctgctCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACaaaaaa                                                                                    >  1:2324753/1‑150 (MQ=255)
                                                                  ctgctCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGATTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACaaaaaaa                                                                                   <  2:1880774/150‑1 (MQ=255)
                                                                      tCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGc                                                                               >  1:2510363/1‑150 (MQ=255)
                                                                      tCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGc                                                                               >  2:316586/1‑150 (MQ=255)
                                                                      tCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGc                                                                               >  2:316757/1‑150 (MQ=255)
                                                                        ccgcgGTATCACCGTCACGTGTGTCGGGGCGACTGCGTGTTCAGCCTCGCTTTCCTCGGGGATTAGCACCTTGCGCAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCgg                                                                             <  1:1065091/149‑1 (MQ=255)
                                                                        aCGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCgg                                                                             >  1:659219/1‑150 (MQ=255)
                                                                         cgcgGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTCAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGc                                                                            >  2:2368600/1‑150 (MQ=255)
                                                                          gcgGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCt                                                                           >  1:1878053/1‑150 (MQ=255)
                                                                              gATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATc                                                                       <  2:2044405/150‑1 (MQ=255)
                                                                                 cACCGTCACGTGTGGAGGGGCTACTGCGTTTTCCGCCTCGGTTTCCGCGCGTGTTAGAACCTTGCGTAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATa                                                                    <  1:1003897/150‑1 (MQ=255)
                                                                                 cACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATa                                                                    >  2:539169/1‑150 (MQ=255)
                                                                                  acggTCACTGGTGGTGGGGCTACTGCTTTTTCAGCCTGGTTTTCCTCGCGGCTTAGAACGTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAg                                                                   <  1:2707782/147‑1 (MQ=255)
                                                                                  aCCGTCACCTGTGGAGGGGCGACTGCTTTTTCAGCCTCGATTTCCTGGCGGCTTAGCACATTGGGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAg                                                                   <  1:2707818/150‑1 (MQ=255)
                                                                                  aCCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAg                                                                   >  1:1593589/1‑150 (MQ=255)
                                                                                          gtgtgGCCGGGCGTCTGCTTGTTCAGCCTCGGTTTCCTCGGGGATTAGCACCTTGCGGAAAATATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGATAATCATAGCTCAGCAt                                                           <  2:2943094/149‑1 (MQ=255)
                                                                                          cTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCAt                                                           >  2:2172196/1‑150 (MQ=255)
                                                                                             tgGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGAc                                                        <  2:123584/150‑1 (MQ=255)
                                                                                             tgGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGAc                                                        <  2:123654/150‑1 (MQ=255)
                                                                                               gccGGGCGACGGCATTTTAAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTC‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGc                                                      <  2:1811079/148‑1 (MQ=255)
                                                                                               gACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGc                                                      >  2:653377/1‑150 (MQ=255)
                                                                                                  gggCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAg                                                                                             <  1:2101617/108‑1 (MQ=255)
                                                                                                   gtctagtGCATGTTCAGCATCTATTTCCTCGGGGTTTATCACCTTGC‑GCAAAAATTACGCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCAttt                                                  <  1:1326928/144‑1 (MQ=255)
                                                                                                   ggCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCAttt                                                  <  1:2860412/150‑1 (MQ=255)
                                                                                                         tGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAAt                                            <  2:1856896/150‑1 (MQ=255)
                                                                                                            cTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGtt                                         >  2:2370837/1‑150 (MQ=255)
                                                                                                            cTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGtt                                         >  1:1851357/1‑150 (MQ=255)
                                                                                                             tGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTg                                        >  1:1933629/1‑150 (MQ=255)
                                                                                                              gTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGa                                       >  2:1895010/1‑150 (MQ=255)
                                                                                                                   gCCTCGCTTTCCTCGCGGCTTCGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTa                                  <  2:1330626/150‑1 (MQ=255)
                                                                                                                   gCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTa                                  >  1:881544/1‑150 (MQ=255)
                                                                                                                   gCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGAAAAAATAA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTa                                  <  1:1789948/150‑1 (MQ=255)
                                                                                                                    ccTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTAc                                 >  1:880452/1‑150 (MQ=255)
                                                                                                                    ccTCGCGTCCCTCGCGGCTTCGAACCTGGCGTAAAAATTTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTAc                                 <  1:65551/150‑1 (MQ=255)
                                                                                                                       cGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAAt                              >  2:2111014/1‑150 (MQ=255)
                                                                                                                         cTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTg                            >  2:2020091/1‑150 (MQ=255)
                                                                                                                          tttCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGc                           >  2:2803986/1‑150 (MQ=255)
                                                                                                                              cTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGAc                       >  2:1993636/1‑150 (MQ=255)
                                                                                                                               tCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACt                      >  1:2320261/1‑150 (MQ=255)
                                                                                                                               tCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACt                      >  1:1152181/1‑150 (MQ=255)
                                                                                                                                 gcgGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCg                    >  1:2230207/1‑150 (MQ=255)
                                                                                                                                  cgGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCgg                   >  1:745986/1‑150 (MQ=255)
                                                                                                                                     attAGCACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCa                <  1:1599008/149‑1 (MQ=255)
                                                                                                                                         gCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggc            >  2:602520/1‑150 (MQ=255)
                                                                                                                                          cACCTTGCTGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcg           <  1:1677716/150‑1 (MQ=255)
                                                                                                                                          cACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcg           <  1:1970621/150‑1 (MQ=255)
                                                                                                                                          cACCTTGCGGAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcg           <  1:115480/150‑1 (MQ=255)
                                                                                                                                           aCCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcgg          >  2:1205264/1‑150 (MQ=255)
                                                                                                                                           aCCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCAcggcgg          >  1:328438/1‑150 (MQ=255)
                                                                                                                                            cggtGCGTCAAAAATTC‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGt         <  1:1569341/147‑1 (MQ=255)
                                                                                                                                            ccTTGCGTAAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGt         <  1:1569232/150‑1 (MQ=255)
                                                                                                                                              ttGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTaa       >  1:1697179/1‑150 (MQ=255)
                                                                                                                                              ttGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTaa       >  1:1697180/1‑150 (MQ=255)
                                                                                                                                              ttGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTaa       >  1:1462173/1‑150 (MQ=255)
                                                                                                                                                   gCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAgccgc  >  2:2601698/1‑150 (MQ=255)
                                                                                                                                                   gCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAgccgc  >  2:1834749/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGCTTTATTGAGCCTGTACATTACCTTCAGGGCATTTTCACTGATATCTTTGCGGGAAATAGCATGCTGCTCACGCGGGATCACCGTCACCTGTGGACGGGCGACTGCCTGTTCAGCCTCGCTTTCCTCGCGGCTTAGCACCTTGCGGCAAAAATTA‑GCGACTCGGGTAAAAATAGTACACCTCGGTAGTGTCAAACATCATTCAGGACAAAAAAATAGCGGCTAATCATAGCTCAGCATGACGCATTTGAGAATGTTGAATTTACAATTGCCGACTCGGGCACGGCGGTAAGCCGC  >  NC_000913/158953‑159249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑