| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,569 | 0 | C | G | 28.2% | 160.3 / 30.4 | 85 | intergenic (+35/‑161) | hrpB/mrcB | putative ATP‑dependent RNA helicase HrpB/peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB |
| Reads supporting (aligned to +/- strand): ref base C (26/35); new base G (0/24); total (26/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.86e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAT > NC_000913/164420‑164715 | gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAATAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGcc > 1:1627762/1‑150 (MQ=255)gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc < 2:2456614/150‑1 (MQ=255)gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCACTGTGGGAGAGGGcc > 1:788287/1‑150 (MQ=255) aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCg < 1:785176/150‑1 (MQ=255) aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 1:336055/150‑1 (MQ=255) aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 1:678767/150‑1 (MQ=255) aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 2:1179291/150‑1 (MQ=255) aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 2:1680823/150‑1 (MQ=255) aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 2:1680915/150‑1 (MQ=255) gCGTACCGTGCGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAGGAATTCGTAAGGTTGGTGTTCTCCCTCTCCCTCTGTGAGAGGGCCGGGG‑TGa > 2:826330/1‑150 (MQ=255) ttACCGTGAGGTGAAAAAAGAGATTAAAGGGCTTTATCCCAAACTTGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAgg < 1:1934027/149‑1 (MQ=39) gTACCGTTAGGTGCAAATAGTGATGAATGGGGGTTATCCCAAACTTGTCTGGCCGTACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAATGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAgg < 2:2841310/150‑1 (MQ=38) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAgg < 2:44915/150‑1 (MQ=255) tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAggg > 2:2720647/1‑150 (MQ=255) aCCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGc > 1:592727/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCAt < 2:2828673/150‑1 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCAt < 2:1126266/150‑1 (MQ=255) gTGAGGTGCAAAAAGAGATGAACGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATc < 2:1487042/150‑1 (MQ=255) tGAGGTGCAAACAGATATGAACGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCa < 2:297531/150‑1 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCa > 2:2268990/1‑150 (MQ=255) gAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAg > 1:2457050/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAgcgc > 1:681707/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCCGCAgcgcg > 1:530307/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTAACCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAgcgcg > 1:1308420/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCAc > 2:1261090/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCAc > 1:502308/1‑150 (MQ=255) aaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACg > 2:1465690/1‑150 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGt > 2:1711096/1‑150 (MQ=255) tttGAATGGGCGTTATCCCAAACAGGTCTGGCGGGACGACCGGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCTTACGGTTGGTTTTCTCCCTCTCCCTGTGGGATCGGGCGGGGT‑TGTGGGCATCAGCGCGCACGTtcacc < 2:2969609/148‑1 (MQ=255) aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACggggggcc > 1:1086738/1‑143 (MQ=255) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGTCGGGG‑TGAGGGCATCAGCGCGCACGTggacccc > 2:1892816/1‑149 (MQ=255) aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCAGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTggacccccc > 1:2365960/1‑147 (MQ=255) aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTtcacccgcacc > 2:1408401/1‑150 (MQ=255) aTCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTGGACCCCCGCCCCaaacac > 1:1495721/1‑146 (MQ=38) aaaccaggTCTGGCCGGACGACCGGGA‑AAATC‑CTGCACCGACGCTACGGCGGAAAAAGTTTTCTAAAGGTTGGTTTGCTCCCTCTCCCGGTGGGTGTGGGCCGGGG‑TGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑Tcc < 2:2576309/143‑1 (MQ=255) aTGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTGGCCCCCCACCCACACCCAC‑TCCCACaa > 2:2743000/1‑150 (MQ=37) ggTCGGGCCGGCCGCCCGGGC‑AAATC‑CTCCCCCGCCGCGAGGCACTAAAATTTATCCGTATGGTTGTTTTCCGCCCTCTCCGTGTGGGAGTGGGCGGGGG‑TGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAg < 2:1493594/149‑1 (MQ=255) gacgggCCGGACGCCCCGGA‑AAATA‑CTGCACCTACGCGACGGACTAAAAAGTATTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAgg < 2:2748185/146‑1 (MQ=37) gggCCGCACGACCGGTC‑AAATA‑GTGCCCCGCCGCCAGGGACTAAAATGTTTTCGAATGGTTGGTTTCCGCCCGCCCCCTGTGGGTGCGGGCCGGGG‑TGTGGCCATCAGCGCGCACGGTCACCCTCACCCTAACCCTC‑TCCCTCAAGGgag < 2:1151501/149‑1 (MQ=255) ggcccGGACCA‑CCGGG‑AAATACCTCCCCCGCCGCGCGGGCCTAAAAATTTTTCGTTGGGTGGTTTTACCCCCCCCCCCGGGGGGAGTGGGCGGGGG‑TGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGgag < 1:2134228/147‑1 (MQ=255) ggCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTATGTTTGGTTTTCTCCCTCCCCCTGGGGGAGAGGGCCGGGG‑TGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTTGAGGgaga < 2:2372280/150‑1 (MQ=255) gCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGATGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGagag < 1:1029469/150‑1 (MQ=255) ccccccgacCCGCA‑AAATA‑CTGCACCGACGCCACGGACGAAAATGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGG‑TGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAgg < 2:2136420/145‑1 (MQ=38) ccGGACGCCCCGGA‑AAATA‑CGGCCCCGACGCGACGGACTAAAATTTTTTCTTAGGTTTGTTTTACTCCCTCCCCCTGTGGGTGTGGGCGGGGG‑TGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAgg < 1:2901503/150‑1 (MQ=255) ccGCACGCCCCCGC‑AAATC‑CGCCCCCGACGGGCGGCACGAAAAAGTATTCGAACGGTTGGTTTTCTCCCTCACCCTGGGGGAGAGGCCCGGGG‑TGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAgg < 1:2901611/150‑1 (MQ=255) cGGACGCCCCGGC‑AATTA‑CGGCCCCGCCGCGACGGACGAAAAACTTTTCGTAAGGTTGGTTTTCCCCCACACCCGGGGGGAGTGGCCCGGGG‑TGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTTGAGGGAGAggg < 2:2555070/150‑1 (MQ=255) cGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTGACCCACACCCAAACCCAA‑ACCCACCACGGAGAggg > 2:1877227/1‑150 (MQ=37) cGGACGACCCGCC‑AAATA‑CTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCACCCGGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAggg < 2:2955863/150‑1 (MQ=255) gtaccccccGGC‑AAATA‑CTGCACCGACGCCCCGCACTAAATTTTTTTCTAATGTTTGTTTTTCACCACCGCCCTGGGGTTGTGGGCGGGGT‑TTGGCGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAgggg < 2:681707/144‑1 (MQ=255) ccgaccGGCAAAATA‑CGCCCCCGACGCGACGGACGAAAAAGTATTCGAACGGTTGTTTTACCCCCACCCCCGGTGGGAGTGGGCGGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGAc < 2:739798/146‑1 (MQ=255) ccGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCCTCAGCGCGCACGTTGGCCCCCACCCCAACCCAC‑AACCCCAACGGGGAGGGGGCCgcac > 1:2821670/1‑147 (MQ=25) gC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTGGACCCGCACCCCAACCCGC‑GCCCCCCAGGGGGGTGGGGCCGCTCgcg > 2:2882355/1‑148 (MQ=25) c‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTGGACCCCCCCCCAAAACCCC‑ACCCGCCAGGGGGAGGGGGCCGATCGAGc > 2:2717309/1‑150 (MQ=25) aaaTA‑CTGCACCGACGCGCCGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGCGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGca < 2:141207/150‑1 (MQ=255) cACCGAGGCGACGTACTAAAATTTTTTGTTATGTTTGGTTTTCGCCCGCCCCGGGGGGGAGTGGGCGGGGG‑TTCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:2514112/150‑1 (MQ=255) cACCGACGCGCCGGACTAAAATGTATTCTTATGGTGGTTTTTCACCCTCGCCGTGTGGGAGGGGGCGGGGG‑TGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:2497164/150‑1 (MQ=255) cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTGGCCCCCACCCAAACCCCCACCCCACAAGGGGGGGGGGCCCCA‑CGAGCAGaaaggga > 2:2338962/1‑146 (MQ=21) ccgccgCGACGGACGAAAAATTCTTCGTACGGTGGGTTTTCTCCCTCTCCCTGGGGGTGAGGGCGGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:1945176/150‑1 (MQ=38) cccacgcgacgGACGAAAAAGATTTCGCAAGGTTGGTTTACTCCCTCCCCCTGTGGGAGAGGGCCGGGG‑TGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:1946079/147‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCTTAAGGTTGGTTTTCTCCCACCCCCTGTGGGAGCGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2580007/150‑1 (MQ=255) ccGACGCGACGGACGAAAAACTATTCGTATGGTTGGTTTACTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:2960556/150‑1 (MQ=255) ccGACCCTACGGACCAAAACTTATTCTTATGTTTGTTTTCCCCCCGCCCCCGGTGGGTGCGGGCGGGGG‑TGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:530307/150‑1 (MQ=255) gacgcgacgGACTAAAAAGTATTGGTATGGTTGTTTTTCTCCCTCTCCCTGGGGGAGTGGGCGGGGG‑TGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 1:2771884/150‑1 (MQ=255) cgcgccgGACGAAAACGTATTCGTACGGTTGGTTTTCTCCCTCGCCCTGTGGGAGAGGGCGGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 2:1636411/150‑1 (MQ=38) cgcgacgGACGAAAAATTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagaga < 2:1636418/150‑1 (MQ=255) gacggacgAACAAGTATTCGAAAGGTTGGTTTACTCCCTCGCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGGGCACAAATTTTGAGAGatat < 2:2648782/150‑1 (MQ=38) aaaatttATTCTTATGGTTGGTTTTCTCCCTCGCCGTGTGGGTTCGGGCCGGGG‑TGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACTGATCGAGCACAAATTTTGAGAGATAtcttcttct < 1:2062950/144‑1 (MQ=255) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCACCGTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACTGATCGAGCACAAATTTTGAGAGATAtcttcttct < 1:2063462/150‑1 (MQ=255) tttttggTATGGTTGTTTTCCCCCCACCCCGGGGGGGTGTGGGCGGGGG‑GGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 1:2268990/144‑1 (MQ=255) tgaTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:1851735/148‑1 (MQ=255) aTTCTTATGTTTGGTTTTCGCCCACCCCCTGGGGGAGTGGGCCGGGG‑TGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 2:592727/150‑1 (MQ=255) tCGTATGGTTGTTTTTCCCCCTCCCCGTGGGGGTGTGGGCGGGGG‑TGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 1:2707455/150‑1 (MQ=255) tgtttgtttTTCTCCCTCTCCCTGGGGTAGTGGGCGGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:1711096/147‑1 (MQ=39) ggttggttTTCTCCCTCGCCCTGTGGGTGTGGGTGGGGG‑TGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag < 1:1892816/150‑1 (MQ=255) gttTTCTCCCTCTCCGTGGGGGAGTGGGCCGGGG‑TGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg < 1:2161598/150‑1 (MQ=255) tctccctctcccTGTGGGAGAGGGCCGGGG‑TGAGGGCCTCAGCGCGCCCGTGGGCCCCCACCCCAACCCAC‑ACCCCCACGGGGGGGGGGGCCGAGCGAGGCCAAAATTTGTGAGAGATATTAATGTGTGTTGTAACACAAGCACAGaaaa > 2:2692559/1‑150 (MQ=12) tccctctccgtGGGGTTGTGGGCGGGGG‑GGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:2502915/150‑1 (MQ=255) cccctctccctGTGGGAGCGGGCGGGGG‑TGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:1014640/149‑1 (MQ=255) cccctcgccctGTGGGTGTGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:2503106/149‑1 (MQ=255) cccctcccccgGGGGTAGTGGGCGGGGG‑TGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 2:1015332/149‑1 (MQ=255) cctctccgtGGGGTTGTGGGCGGGGG‑TGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:561415/150‑1 (MQ=255) cgtgtgGTAGCGGGCGGGGG‑TGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt < 2:502308/148‑1 (MQ=255) ctGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCATAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg > 1:766155/1‑150 (MQ=255) gttgcgggCGGGGG‑TGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:1864600/145‑1 (MQ=255) ggtgtgggCGGGGG‑TGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:1864605/145‑1 (MQ=255) tgtggggCCGGGGTTGGGGGCATCTGCTCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGTGGGGACCTATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:826330/146‑1 (MQ=255) tgcgggCGGGGG‑GGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGa < 1:2055802/147‑1 (MQ=255) ggCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAATCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCGGAatat > 2:819045/1‑150 (MQ=255) | GAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGC‑AAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGG‑TGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTC‑TCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATAT > NC_000913/164420‑164715 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |