Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913361,3200CA21.8% 497.5 / 20.9 188V181F (GTT→TTT) lacAgalactoside O‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base C (49/98);  new base A (41/0);  total (90/98)
Fisher's exact test for biased strand distribution p-value = 1.56e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 197 total aligned reads displayed.

TTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCG  >  NC_000913/361171‑361469
                                                                                                                                                     |                                                                                                                                                      
ttCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAc                                                                                                                                                        <  2:969949/150‑1 (MQ=255)
ttCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAc                                                                                                                                                        <  2:1335273/150‑1 (MQ=255)
      ccGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACa                                                                                                                                                  <  1:254802/150‑1 (MQ=255)
       cGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACaa                                                                                                                                                 <  2:2674720/150‑1 (MQ=255)
         gATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAgg                                                                                                                                               <  2:1061076/150‑1 (MQ=255)
              ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGc                                                                                                                                          <  2:589413/150‑1 (MQ=255)
              ggCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGc                                                                                                                                          >  2:717605/1‑150 (MQ=255)
                cTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCa                                                                                                                                        <  2:100110/150‑1 (MQ=255)
                cTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCa                                                                                                                                        <  2:2207654/150‑1 (MQ=255)
                 tAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAACGAACGCCac                                                                                                                                       >  1:541172/1‑149 (MQ=255)
                  aaTGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAgc                                                                                                                                      <  2:2048909/150‑1 (MQ=255)
                   aTGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAAAAAACCGAAAATGAACCCCacac                                                                                                                                     >  2:1219152/1‑147 (MQ=38)
                     gTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAgccgc                                                                                                                                   <  2:1703607/150‑1 (MQ=255)
                      tAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGCATAAACCCACAAGGAACGCCAgccgcc                                                                                                                                  >  1:938724/1‑150 (MQ=39)
                      tAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACACCAgccccc                                                                                                                                  >  2:1525964/1‑150 (MQ=255)
                      tAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAgccgcc                                                                                                                                  >  2:1609732/1‑150 (MQ=255)
                         aTCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAACGCCACCCGCCacc                                                                                                                               >  1:1678516/1‑149 (MQ=255)
                           cGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCacgac                                                                                                                             <  1:2482897/150‑1 (MQ=255)
                             cTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGt                                                                                                                           <  1:1668959/150‑1 (MQ=255)
                               gAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAAAAACCCGACAAGGAACGCCAGCCCCCACGACGttt                                                                                                                         >  2:1544678/1‑150 (MQ=255)
                                   ttGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggtg                                                                                                                     <  1:2911481/150‑1 (MQ=255)
                                   ttGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggtg                                                                                                                     <  2:320889/150‑1 (MQ=255)
                                    tGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTtggtgg                                                                                                                    <  1:2146101/150‑1 (MQ=255)
                                     gTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGa                                                                                                                   <  2:739064/150‑1 (MQ=255)
                                          ccTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGc                                                                                                                                          >  2:649514/1‑122 (MQ=255)
                                          ccTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGc                                                                                                                                          <  1:649514/122‑1 (MQ=255)
                                          ccTGATACGCTGCGCTTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTAGCGTATAACCCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGAATgga                                                                                                              >  2:537983/1‑148 (MQ=255)
                                           cTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTGGGGAATAACCCGACAAGGAACGCCAGCCGCCACGGCGGTtggtggtctgtgt                                                                                                             >  2:2834189/1‑143 (MQ=255)
                                            tGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtt                                                                                                            >  2:1504987/1‑150 (MQ=255)
                                              aTAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTGATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGGCAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCtttt                                                                                                          <  2:1666810/150‑1 (MQ=255)
                                                 aGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACGCCAGCCGCCACCACGTTTGGTGGAATTTATTTtgtg                                                                                                       >  2:96253/1‑150 (MQ=255)
                                                  gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑a                                                                                                     <  1:73356/150‑1 (MQ=255)
                                                  gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAAAGCCACCCGCCACCAAGTTAGGTGGAATGTATTTtgtta                                                                                                      >  2:217903/1‑148 (MQ=255)
                                                  gcgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCGAATAACCCCACAAGGAACGCCACCCGCCACGACGTTTGGTGGAATGTAATTtgtgt                                                                                                      >  2:2532542/1‑149 (MQ=255)
                                                   cgcAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑Ac                                                                                                    <  1:1791306/150‑1 (MQ=255)
                                                      aGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGAt                                                                                                 <  1:2261114/150‑1 (MQ=255)
                                                          tATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTTTTTGGGGAATAAACCCACAACGAAAGCCAGCCCCCACCCCCTTTGGTTGTATTTGTTTTTTG‑TGGAtaaaa                                                                                             >  1:6868/1‑147 (MQ=255)
                                                            tCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGCACGCCAGCCGCCACGACGGTTGGTGGAATGTCTTTTTTG‑TCGataataac                                                                                           >  2:181586/1‑148 (MQ=255)
                                                            tCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACACGACAACGAACGCCACCCGCCACCACGTTTGGTGGAAATTAGTTTTTG‑ACGataataac                                                                                           >  2:1873556/1‑148 (MQ=255)
                                                             cAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCCCCACCACGTATGGTGGAATGTCTTTTTTG‑ACGATAATTccc                                                                                          >  2:2874344/1‑150 (MQ=255)
                                                                gCAATTTTTATAATTTAAATTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAAAGCCCGCCGCCACGACGGTTGGTGGAATGTCTTTTGTG‑ACGATACAACCcgcg                                                                                       >  2:2133472/1‑150 (MQ=255)
                                                                    tttttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCaa                                                                                   <  1:1178022/150‑1 (MQ=255)
                                                                       ttATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATaa                                                                                <  1:1637961/150‑1 (MQ=255)
                                                                        tataATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTTTG‑AAGATAATACCCGCGCCAATAAc                                                                               >  2:1878397/1‑150 (MQ=255)
                                                                           aaTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAACCCCACAAGGGACGCCCCCCGCCACCCCGGTTGGGGGTATGTCTTTTTTG‑TAGATACTAACCCCGCCAaaaaaaca                                                                            >  1:2290082/1‑146 (MQ=255)
                                                                            aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCCACAAGGTAAGCCAGCCGCCCCGACGGTTGGTGGTATGTGGTTTTTT‑TAGATAAAACCCCCGCCAaaaaaaaaa                                                                           >  1:1481969/1‑145 (MQ=255)
                                                                            aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGaa                                                                           <  2:543743/150‑1 (MQ=255)
                                                                            aTTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCGACAAGGAACGCCAGCCGCCACCACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTAACCGCGCCAATAAAAGaa                                                                           >  2:2769983/1‑150 (MQ=255)
                                                                                 aaCTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATc                                                                      <  2:334668/150‑1 (MQ=255)
                                                                                    tGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAATAAACCCACAACGTACCCCAGCCCCCCCCACGGTTGGTGGGCTGTGTTTTTTG‑TCGATACAAACCCCCCCCAAAAAAGAAatataacc                                                                   >  1:250499/1‑146 (MQ=255)
                                                                                      aCGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGa                                                                 <  1:1060667/150‑1 (MQ=255)
                                                                                        gATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAACCCCACAAGGAAAGCCAGCCCCCACCACGGTTTGTGGAAATTATTTTGTG‑AAGATAATAACCCCCCCAAAAACAGAAATATaccccagg                                                               >  2:388738/1‑146 (MQ=255)
                                                                                        gATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTGTATTTAGCCACTAACCCGACAATGAACGCCAGCCCCCACCACGGTTGGTGGAATTTCGTTTGTG‑ACGATACTACCCCCGCCAATAACAGAAATATACCCGCTg                                                               >  2:2731809/1‑150 (MQ=255)
                                                                                           tCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAACGCCAGCCCCCCCCACGGTTGGTGGTAAGTATTTTTTG‑TCGATAAAAACCCCGCCCAAAAAAGAAATATaccccaatgtg                                                            >  1:2068275/1‑143 (MQ=255)
                                                                                             aaCTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTAGCGAAAAAACCCACAAGGAAACCCAGCCGCCCCCACGTTTGGTGGTAAGTATTTTTTG‑TCGATAATAACCCCCCCCAAAAAATAAATATACCCCAAGGTGAc                                                          >  1:1916819/1‑150 (MQ=255)
                                                                                               cTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGc                                                        <  1:1265606/150‑1 (MQ=255)
                                                                                                  tataATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTg                                                     <  1:1896111/150‑1 (MQ=255)
                                                                                                    taATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGa                                                   <  2:779559/150‑1 (MQ=255)
                                                                                                     aaTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTTTTTCGCGAATAAACCCACAAGGAACGCCAGCCCCCACGACGTTTGGTGGAAATTATTTTTTG‑AAGATAAAACCCCCGCCAAAAAAAAAAATATACCCCAAGGTGACGCCAGGAt                                                  >  1:2225820/1‑150 (MQ=255)
                                                                                                     aaTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTTGCGAATAAAACCACAACGAACCCCACCCCCCCCCACGGTTGGTGGGATGTCTTTTGTT‑TAGCTACAACCCGCGCCCAAAAAACAAATATACCCCAAGGTGAAGCCTgggt                                                  >  1:815526/1‑148 (MQ=255)
                                                                                                      aTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGAtt                                                 <  2:328889/150‑1 (MQ=255)
                                                                                                      aTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGAtt                                                 <  2:1846466/150‑1 (MQ=255)
                                                                                                      aTCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTAGCCAATAACCCGACAACGAAACCCCGCCGCCACGACGTTTGGTGGAATGTCTTTTGTGCACCA‑ACAACCCCCGCCCAAAAAAGAATTAACCCCCCTGGTGACGCCGGGGtt                                                 >  2:1457739/1‑150 (MQ=255)
                                                                                                            gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAAc                                           <  2:1388764/150‑1 (MQ=255)
                                                                                                            gAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAAACCGACAAGGAACGCCAGCCGCCACGACGGTTGGTGGTATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAAc                                           >  2:2308651/1‑150 (MQ=255)
                                                                                                             aaaTAATAGTGCTTATCCCGGTCGTTTATTTAGCGAATAAACCCACAAGGAACGCCAGCCGCCACGAAGGTTGGTGGAATTTCTTTTGTT‑ACGATAAAACCCGCGCCAATAAAAGAATTATCCCCGATGGTGACGCCTGGATtaaaaaaa                                          >  2:2609138/1‑148 (MQ=255)
                                                                                                              aataatAGTGCTTATCCCGGTCATTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACca                                         <  2:82345/150‑1 (MQ=255)
                                                                                                                 aataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACAt                                      <  2:616150/150‑1 (MQ=255)
                                                                                                                 aataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACAt                                      <  1:2649830/150‑1 (MQ=255)
                                                                                                                 aataGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGCCCGCCGCCACGACGGTTGGTGGTATGTGTTTTGTG‑ACGATAATACCCGCGCCAATAACAGAAATATACCCCATGGTGACGCCGGGATTAATAACCACAt                                      >  2:1136112/1‑150 (MQ=255)
                                                                                                                       gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                                <  1:912613/150‑1 (MQ=255)
                                                                                                                       gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                                <  1:181586/150‑1 (MQ=255)
                                                                                                                       gCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTc                                <  1:120733/150‑1 (MQ=255)
                                                                                                                              cccgTCGTTTATTTCGCGAATCACCCGACAAGGAACGCCACCCGCCACGACGTTTGGTGGAATGTCTTTGGTG‑ACGATACGACCCGCGCCACTAACAGAATTAACCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCa                         <  2:1067784/147‑1 (MQ=255)
                                                                                                                              ccGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCa                         <  1:2016657/150‑1 (MQ=255)
                                                                                                                              ccGGTCGTTTATTTCGCGAATAACCCCACAAGGAACGCCAGCCCCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCCCGCCAAAAACAGAATTATACCCGATGGTGACGCCAGGATTAATAACCACAAGACTTACGAACCa                         >  2:205626/1‑150 (MQ=255)
                                                                                                                                  tCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACg                     >  2:1927786/1‑150 (MQ=255)
                                                                                                                                    gTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGtt                   <  2:449526/150‑1 (MQ=255)
                                                                                                                                       tATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTAtt                <  1:2409695/150‑1 (MQ=255)
                                                                                                                                       tATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTAtt                <  2:2919549/150‑1 (MQ=255)
                                                                                                                                       tATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTAtt                >  1:602142/1‑150 (MQ=255)
                                                                                                                                       tATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTAtt                <  1:464840/150‑1 (MQ=255)
                                                                                                                                        aTTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTg               <  2:1024478/150‑1 (MQ=255)
                                                                                                                                        aTTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTg               >  1:1357778/1‑150 (MQ=255)
                                                                                                                                         tttCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGc              <  1:273819/150‑1 (MQ=255)
                                                                                                                                         tttCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGc              <  2:689169/150‑1 (MQ=255)
                                                                                                                                           tCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCa            >  1:1323585/1‑150 (MQ=255)
                                                                                                                                            cgcgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCaa           >  1:658076/1‑150 (MQ=255)
                                                                                                                                             gcgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAAt          >  1:1521493/1‑150 (MQ=255)
                                                                                                                                             gcgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAAt          >  1:1444362/1‑150 (MQ=255)
                                                                                                                                              cgAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATc         <  2:946969/150‑1 (MQ=255)
                                                                                                                                               gAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCg        >  1:43455/1‑150 (MQ=255)
                                                                                                                                               gAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCg        <  2:1176168/150‑1 (MQ=255)
                                                                                                                                                 aTAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGtt      <  1:877022/150‑1 (MQ=255)
                                                                                                                                                 aTAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGtt      >  2:1289232/1‑150 (MQ=255)
                                                                                                                                                  tAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTa     >  2:46031/1‑150 (MQ=255)
                                                                                                                                                    aCCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATc   <  2:1247040/150‑1 (MQ=255)
                                                                                                                                                     cccGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCg  >  1:761518/1‑150 (MQ=255)
                                                                                                                                                     cccGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCg  >  1:757421/1‑150 (MQ=255)
                                                                                                                                                     cccGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCg  <  2:2487695/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
TTCATGCCGGATGCGGCTAATGTAGATCGCTGAACTTGTAGGCCTGATAAGCGCAGCGTATCAGGCAATTTTTATAATTTAAACTGACGATTCAACTTTATAATCTTTGAAATAATAGTGCTTATCCCGGTCGTTTATTTCGCGAATAACCCGACAAGGAACGCCAGCCGCCACGACGTTTGGTGGAATGTCTTTTGTG‑ACGATACTACCCGCGCCAATAACAGAATTATCCCCGATGGTGACGCCTGGATTAATAACCACATGACTTCCGATCCAGACGTTATTGCCAATCGTTATCG  >  NC_000913/361171‑361469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑